2. Academic Validation
  3. ZSWIM1 Promotes the Proliferation and Metastasis of Lung Adenocarcinoma Cells through the STK38/MEKK2/ERK1/2 Axis

ZSWIM1 Promotes the Proliferation and Metastasis of Lung Adenocarcinoma Cells through the STK38/MEKK2/ERK1/2 Axis

  • J Proteome Res. 2022 Dec 13. doi: 10.1021/acs.jproteome.2c00412.
Xuejuan Gao 1,  Qionghua Lian 1,  Baiye Guan 1,  Qiu-Yu Liu 2,  Meng Meng 1,  Yang Chen 1,  Jingjie Jin 1,  Huihua Li 1,  Xiaohui Liu 1,  Zhenghua Sun 1,  Langxia Liu 1,  Qing-Yu He 1,  Gong Zhang 1
Affiliations

Affiliations

  • 1 MOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
  • 2 Department of Pathology, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003, China.
PMID: 36511424 DOI: 10.1021/acs.jproteome.2c00412
Abstract

Investigating the functions of the proteins with no or less functional annotations is an important goal of the HPP (Human Proteome Project) Grand Project. In this study, we investigated the function of such a protein, ZSWIM1 (C20orf162), its gene located on chromosome 20. Its expression is upregulated in lung adenocarcinoma compared with the adjacent normal tissues and negatively correlated with the overall survival. Overexpressing ZSWIM1 markedly promotes the proliferation, migration, invasion as well as epithelial-to-mesenchymal transition in lung adenocarcinoma cells, while knocking down ZSWIM1 functions oppositely. The interactome of ZSWIM1 was identified by immunoprecipitation-mass spectrometry, and we verified the interaction of ZSWIM1 with the potential partner, STK38. ZSWIM1 antagonized the function of STK38. Mechanically, ZSWIM1 promoted the activation of MEKK2/ERK1/2 pathway through interacting with STK38, leading to the release of MEKK2. Taken together, ZSWIM1 can be annotated as an oncogene in lung adenocarcinoma, and the STK38/MEKK2/ERK1/2 axis mediates its promoting role in lung adenocarcinoma.

Keywords

ERK1/2; STK38; ZSWIM1; lung adenocarcinoma; migration; proliferation.

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