2. Academic Validation
  3. Development of a spermine lipid for transient antibody expression

Development of a spermine lipid for transient antibody expression

  • Bioorg Med Chem. 2023 Jan 15:78:117114. doi: 10.1016/j.bmc.2022.117114.
Shaowei Sui 1,  Hao Wang 1,  Jiajie Song 1,  Wanyi Tai 2
Affiliations

Affiliations

  • 1 Key Laboratory of Combinatorial Biosynthesis and Drug Discovery Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan, Hubei 430071, China.
  • 2 Key Laboratory of Combinatorial Biosynthesis and Drug Discovery Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan, Hubei 430071, China; Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China. Electronic address: [email protected].
PMID: 36563514 DOI: 10.1016/j.bmc.2022.117114
Abstract

Transient expression is the only way to quickly obtain a small scale of antibodies for biomedical research and therapeutic evaluation. The agents for transfecting the suspension cells, e.g. PEI or commercial agents, either lack efficiency or excessively expensive. Herein, a novel spermine-based lipid was developed and fabricated into a cationic Liposome for antibody expression. This new transfection agent, designated as sperminoliposome, is feasible, cheap, and highly effective to produce antibodies. Compared to PEI, a 3 times higher yield of antibody was obtained by sperminoliposome during the transient expression of cetuximab in suspension 293F cells. Characterizations confirmed that the expressed antibody is fully functional and eligible for further research. Our study provides an effective tool for the rapid production of antibodies economically and feasibly.

Keywords

Antibody expression; Cationic lipid; Cetuximab; Spermine; Transient transfection.

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