1. Academic Validation
  2. Multifunctional Electrospinning Polyhydroxyalkanoate Fibrous Scaffolds with Antibacterial and Angiogenesis Effects for Accelerating Wound Healing

Multifunctional Electrospinning Polyhydroxyalkanoate Fibrous Scaffolds with Antibacterial and Angiogenesis Effects for Accelerating Wound Healing

  • ACS Appl Mater Interfaces. 2022 Dec 28. doi: 10.1021/acsami.2c16905.
Jian Li 1 2 Jiang-Nan Chen 3 4 Zi-Xin Peng 1 Ning-Bo Chen 5 Cheng-Bo Liu 5 Peng Zhang 1 Xu Zhang 4 6 Guo-Qiang Chen 3 4 6
Affiliations

Affiliations

  • 1 Shenzhen Engineering Research Center for Medical Bioactive Materials, Center for Translational Medicine Research & Development, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong 518055, China.
  • 2 University of Chinese Academy of Sciences, Beijing 100049, China.
  • 3 School of Life Sciences, Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China.
  • 4 Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China.
  • 5 Research Laboratory for Biomedical Optics and Molecular Imaging, CAS Key Laboratory of Health Informatics, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
  • 6 Key Laboratory of Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
Abstract

To treat large-scale wounds or chronic ulcers, it is highly desirable to develop multifunctional wound dressings that integrate Antibacterial and angiogenic properties. While many biomaterials have been fabricated as wound dressings for skin regeneration, few reports have addressed the issue of complete skin regeneration due to the lack of vasculature and hair follicles. Herein, an instructive poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P34HB) fibrous wound dressing that integrates an Antibacterial ciprofloxacin (CIP) and pro-angiogenic dimethyloxalylglycine (DMOG) is successfully prepared via electrospinning. The resultant dressings exhibit suitable flexibility with tensile strength and elongation at break up to 4.08 ± 0.18 MPa and 354.8 ± 18.4%, respectively. The in vitro results revealed that the groups of P34HB/CIP/DMOG dressings presented excellent biocompatibility on cell proliferation and significantly promote the spread and migration of L929 cells in both transwell and scratch assays. Capillary-like tube formation is also significantly enhanced in the P34HB/CIP/DMOG group dressings. Additionally, dressings from the P34HB/CIP and P34HB/CIP/DMOG groups show a broad spectrum of antimicrobial action against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. In vivo studies further demonstrated that the prepared dressings in the P34HB/CIP/DMOG group not only improved wound closure, increased re-epithelialization and collagen formation, as well as reduced inflammatory response but also increased angiogenesis and remodeling, resulting in complete skin regeneration and hair follicles. Collectively, this work provides a simple but efficient approach for the design of a versatile wound dressing with the potential to have a synergistic effect on the rapid stimulation of angiogenesis as well as Antibacterial activity in full-thickness skin repair.

Keywords

PHA; PHB; angiogenesis; antibacteria; electrospinning; polyhydroxyalkanoates; wound healing.

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