2. Metabolic Enzyme/Protease
    Autophagy
  3. HIF/HIF Prolyl-Hydroxylase
    Autophagy
  4. DMOG

DMOG  (Synonyms: Dimethyloxallyl Glycine)

Cat. No.: HY-15893 Purity: 99.77%
COA Handling Instructions

DMOG (diméthyloxallyl glycine) est un inhibiteur perméable aux cellules et compétitif de HIF-PH, ce qui entraîne une stabilisation et une accmulation de HIF-1α in vitro et in vivo. DMOG est un analogue de l'α-cétoglutarate et inhibe les hydroxylases dépendantes de l'α-KG. DMOG agit comme un agent pro-angiogénique et joue un rôle protecteur dans le modèle expérimental de colite et de diarrhée via le signal lié à HIF-1. DMOG induit l'autophagie cellulaire.

DMOG (Dimethyloxallyl Glycin) ist ein zellpermeabler und kompetitiver Inhibitor von HIF-PH, der in vitro und in vivo zur Stabilisierung und Akkmulation von HIF-1α führt. DMOG ist ein α-Ketoglutarat-Analogon und hemmt α-KG-abhängige Hydroxylasen. DMOG wirkt als pro-angiogener Wirkstoff und spielt über das HIF-1-bezogene Signal eine protektive Rolle im experimentellen Modell von Kolitis und Diarrhoe. DMOG induziert die autophagy.

DMOG (Dimethyloxallyl Glycine) is a cell permeable and competitive inhibitor of HIF-PH, which results in HIF-1α stabilisation and accmulation in vitro and in vivo. DMOG is an α-ketoglutarate analogue and inhibits α-KG-dependent hydroxylases. DMOG acts as a pro-angiogenic agent and plays a protective role in experimental model of colitis and diarrhoea via HIF-1 related signal. DMOG induces cell autophagy.

For research use only. We do not sell to patients.

DMOG Chemical Structure

DMOG Chemical Structure

CAS No. : 89464-63-1

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Customer Review

Based on 38 publication(s) in Google Scholar

Top Publications Citing Use of Products

36 Publications Citing Use of MCE DMOG

WB

    DMOG purchased from MCE. Usage Cited in: Front Immunol. 2018 Jul 23;9:1667.  [Abstract]

    Manipulation of the protein level of hypoxia-inducible factor 1 alpha (HIF-1α) in NR8383. 50 µM PX478 treatment for 20 h is used to downregulate the HIF-1α protein level in NR8383 cells (A). 1 mM DMOG treatment for 8 h is used to upregulate the HIF-1α protein level in NR8383 cells (B).

    DMOG purchased from MCE. Usage Cited in: J Biol Chem. 2015 Jan 16;290(3):1389-94.  [Abstract]

    The hypoxia induced the expression of NEK8. Cells are treated with CoCl2 (100 μM) or DMOG (100 μM) for 6 h or cultured for 12 h in hypoxia (1.0% oxygen). HIF-1α and NEK8 are analyzed by immunoblotting, GAPDH is employed as a loading control.

    • Biological Activity

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    • References

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    Description

    DMOG (Dimethyloxallyl Glycine) is a cell permeable and competitive inhibitor of HIF-PH, which results in HIF-1α stabilisation and accmulation in vitro and in vivo[1]. DMOG is an α-ketoglutarate analogue and inhibits α-KG-dependent hydroxylases. DMOG acts as a pro-angiogenic agent and plays a protective role in experimental model of colitis and diarrhoea via HIF-1 related signal[2][4]. DMOG induces cell autophagy[5].

    IC50 & Target

    HIF-1α prolyl hydroxylase[1]
    In Vitro

    DMOG efficiently suppresses hydroxyproline synthesis in intact cells, but shows only weakly active in the microsomal system[1]. DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    In Vivo

    DMOG inhibits endogenous HIF inactivation, and induces angiogenesis in ischaemic skeletal muscles of mice[2]. Up-regulation of hypoxia-inducible factor-1α by DMOG enhances the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    175.14

    Appearance

    Solid

    Formula

    C6H9NO5
    CAS No.
    SMILES

    O=C(OC)CNC(C(OC)=O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 50 mg/mL (285.49 mM)

    H2O : 50 mg/mL (285.49 mM; Need ultrasonic)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.7097 mL 28.5486 mL 57.0972 mL
    5 mM 1.1419 mL 5.7097 mL 11.4194 mL
    10 mM 0.5710 mL 2.8549 mL 5.7097 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  PBS

      Solubility: 150 mg/mL (856.46 mM); Clear solution; Need ultrasonic

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 99.77%

    COA (244 KB) LCMS (268 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [3]

    To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [2]

    Two groups of mice (C57Bl6) are used. One group (n=11) receives dimethyloxalylglycine (DMOG) i.p. (8 mg in 0.5 mL saline) on days 1, 3, 5, 7 and 9 while the animals in the other group are injected with sterile saline (0.5 mL) at the same intervals (n=6). A third group is treated with DMOG without ligation (n=4) and four unoperated mice serve as controls. After 11 days mice are terminally anaesthetized and the extensor digitorum longus (EDL) and tibialis anterior (TA) muscles excised.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Keywords:

    DMOG89464-63-1Dimethyloxallyl GlycineHIF/HIF Prolyl-HydroxylaseAutophagyHypoxia-inducible factorsHIFsHIF-PH diarrhoeastabilisationaccmulationpro-angiogenicInhibitorinhibitorinhibit

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