2. Metabolic Enzyme/Protease Autophagy
  3. HIF/HIF Prolyl-Hydroxylase Autophagy
  4. DMOG

DMOG  (Synonyms: Dimethyloxallyl Glycine)

Cat. No.: HY-15893 Purity: 99.69%
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DMOG (diméthyloxallyl glycine) est un inhibiteur perméable aux cellules et compétitif de HIF-PH, ce qui entraîne une stabilisation et une accmulation de HIF-1α in vitro et in vivo. DMOG est un analogue de l'α-cétoglutarate et inhibe les hydroxylases dépendantes de l'α-KG. DMOG agit comme un agent pro-angiogénique et joue un rôle protecteur dans le modèle expérimental de colite et de diarrhée via le signal lié à HIF-1. DMOG induit l'autophagie cellulaire.

DMOG (Dimethyloxallyl Glycin) ist ein zellpermeabler und kompetitiver Inhibitor von HIF-PH, der in vitro und in vivo zur Stabilisierung und Akkmulation von HIF-1α führt. DMOG ist ein α-Ketoglutarat-Analogon und hemmt α-KG-abhängige Hydroxylasen. DMOG wirkt als pro-angiogener Wirkstoff und spielt über das HIF-1-bezogene Signal eine protektive Rolle im experimentellen Modell von Kolitis und Diarrhoe. DMOG induziert die autophagy.

DMOG (Dimethyloxallyl Glycine) is a cell permeable and competitive inhibitor of HIF-PH, which results in HIF-1α stabilisation and accmulation?in vitro and in vivo. DMOG is an α-ketoglutarate analogue and inhibits α-KG-dependent hydroxylases. DMOG?acts as a pro-angiogenic agent and plays a protective role in experimental model of colitis and diarrhoea via HIF-1 related signal. DMOG induces cell autophagy.

For research use only. We do not sell to patients.

DMOG Chemical Structure

DMOG Chemical Structure

CAS No. : 89464-63-1

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Customer Review

Based on 55 publication(s) in Google Scholar

DMOG Related Antibodies

Top Publications Citing Use of Products

54 Publications Citing Use of MCE DMOG

WB

    DMOG purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2015 Jan 16;290(3):1389-94.  [Abstract]

    The hypoxia induced the expression of NEK8. Cells are treated with CoCl2 (100 μM) or DMOG (100 μM) for 6 h or cultured for 12 h in hypoxia (1.0% oxygen). HIF-1α and NEK8 are analyzed by immunoblotting, GAPDH is employed as a loading control.

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    HIF-1α HIF

    • Biological Activity

    • Protocol

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    • References

    • Customer Review

    Description

    DMOG (Dimethyloxallyl Glycine) is a cell permeable and competitive inhibitor of HIF-PH, which results in HIF-1α stabilisation and accmulation?in vitro and in vivo[1]. DMOG is an α-ketoglutarate analogue and inhibits α-KG-dependent hydroxylases. DMOG?acts as a pro-angiogenic agent and plays a protective role in experimental model of colitis and diarrhoea via HIF-1 related signal[2][4]. DMOG induces cell autophagy[5].

    IC50 & Target

    HIF-1α prolyl hydroxylase[1]
    In Vitro

    DMOG efficiently suppresses hydroxyproline synthesis in intact cells, but shows only weakly active in the microsomal system[1].?DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DMOG Related Antibodies
    In Vivo

    DMOG inhibits endogenous HIF inactivation, and induces angiogenesis in ischaemic skeletal muscles of mice[2]. Up-regulation of hypoxia-inducible factor-1α by DMOG enhances the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    175.14

    Formula

    C6H9NO5
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(OC)CNC(C(OC)=O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (570.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 50 mg/mL (285.49 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.7097 mL 28.5486 mL 57.0972 mL
    5 mM 1.1419 mL 5.7097 mL 11.4194 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 150 mg/mL (856.46 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.69%

    SDS (562 KB)

    COA (244 KB) HNMR (132 KB) RP-HPLC (247 KB) LCMS (104 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [3]

    To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [2]

    Two groups of mice (C57Bl6) are used. One group (n=11) receives dimethyloxalylglycine (DMOG) i.p. (8 mg in 0.5 mL saline) on days 1, 3, 5, 7 and 9 while the animals in the other group are injected with sterile saline (0.5 mL) at the same intervals (n=6). A third group is treated with DMOG without ligation (n=4) and four unoperated mice serve as controls. After 11 days mice are terminally anaesthetized and the extensor digitorum longus (EDL) and tibialis anterior (TA) muscles excised.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 5.7097 mL 28.5486 mL 57.0972 mL 142.7429 mL
    5 mM 1.1419 mL 5.7097 mL 11.4194 mL 28.5486 mL
    10 mM 0.5710 mL 2.8549 mL 5.7097 mL 14.2743 mL
    15 mM 0.3806 mL 1.9032 mL 3.8065 mL 9.5162 mL
    20 mM 0.2855 mL 1.4274 mL 2.8549 mL 7.1371 mL
    25 mM 0.2284 mL 1.1419 mL 2.2839 mL 5.7097 mL
    30 mM 0.1903 mL 0.9516 mL 1.9032 mL 4.7581 mL
    40 mM 0.1427 mL 0.7137 mL 1.4274 mL 3.5686 mL
    50 mM 0.1142 mL 0.5710 mL 1.1419 mL 2.8549 mL
    60 mM 0.0952 mL 0.4758 mL 0.9516 mL 2.3790 mL
    80 mM 0.0714 mL 0.3569 mL 0.7137 mL 1.7843 mL
    100 mM 0.0571 mL 0.2855 mL 0.5710 mL 1.4274 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    DMOG Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DMOG89464-63-1Dimethyloxallyl GlycineHIF/HIF Prolyl-HydroxylaseAutophagyHypoxia-inducible factorsHIFsHIF-PHdiarrhoeastabilisationaccmulationpro-angiogenicInhibitorinhibitorinhibit

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