1. Metabolic Enzyme/Protease
  2. HIF/HIF Prolyl-Hydroxylase

DMOG (Synonyms: Dimethyloxallyl Glycine)

Cat. No.: HY-15893 Purity: >98.0%
Data Sheet SDS Handling Instructions

DMOG is an inhibitor of HIF prolyl hydroxylase and an antagonist of α-ketoglutarate cofactor.

For research use only. We do not sell to patients.
DMOG Chemical Structure

DMOG Chemical Structure

CAS No. : 89464-63-1

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10 mM * 1 mL in DMSO $66 In-stock
50 mg $60 In-stock
100 mg $110 In-stock
200 mg $190 In-stock
500 mg $440 In-stock
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Customer Review

    DMOG purchased from MCE. Usage Cited in: Cancer Biol Ther. 2016 Jun 2;17(6):625-34.

    HL-60 and Kasumi-1 cells are treated with different concentration of 2ME2 (0.1, 0.5, 2.5, or 5μM) for 24 hours or diluent control, and intracelluar protein levels are examined by Western blot.

    DMOG purchased from MCE. Usage Cited in: J Biol Chem. 2015 Jan 16;290(3):1389-94.

    The hypoxia induced the expression of NEK8. Cells are treated with CoCl2 (100 μM) or DMOG (100 μM) for 6 h or cultured for 12 h in hypoxia (1.0% oxygen). HIF-1α and NEK8 are analyzed by immunoblotting, GAPDH is employed as a loading control.

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    • Biological Activity

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    • References

    Description

    DMOG is an inhibitor of HIF prolyl hydroxylase and an antagonist of α-ketoglutarate cofactor.

    In Vitro

    DMOG efficiently suppresses hydroxyproline synthesis in intact cells, but shows only weakly active in the microsomal system[1]. DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC[3].

    In Vivo

    DMOG inhibits endogenous HIF inactivation, and induces angiogenesis in ischaemic skeletal muscles of mice[2]. Up-regulation of hypoxia-inducible factor-1α by DMOG enhances the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats[4]

    References
    Preparing Stock Solutions
    Concentration Volume Mass 1 mg 5 mg 10 mg
    1 mM 5.7097 mL 28.5486 mL 57.0972 mL
    5 mM 1.1419 mL 5.7097 mL 11.4194 mL
    10 mM 0.5710 mL 2.8549 mL 5.7097 mL
    Please refer to the solubility information to select the appropriate solvent.
    Cell Assay
    [3]

    To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [2]

    DMOG is dissolved in sterile saline.

    Two groups of mice (C57Bl6) are subjected to unilateral femoral artery ligation under fluothane inhalation anaesthesia (2% in O2). One group (n=11) receives dimethyloxalylglycine (DMOG) i.p. (8 mg in 0.5 mL saline) on days 1, 3, 5, 7 and 9 while the animals in the other group are injected with sterile saline (0.5 mL) at the same intervals (n=6). A third group is treated with DMOG without ligation (n=4) and four unoperated mice serve as controls. After 11 days mice are terminally anaesthetized and the extensor digitorum longus (EDL) and tibialis anterior (TA) muscles excised. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    175.14

    Formula

    C₆H₉NO₅

    CAS No.

    89464-63-1

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Solvent & Solubility

    DMSO: 10 mM

    * "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

    Purity: >98.0%

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    Product Name:
    DMOG
    Cat. No.:
    HY-15893
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