1. Metabolic Enzyme/Protease
  2. HIF/HIF Prolyl-Hydroxylase

DMOG (Synonyms: Dimethyloxallyl Glycine)

Cat. No.: HY-15893 Purity: 99.15%
Handling Instructions

DMOG (Dimethyloxallyl Glycine) is a cell-permeable and competitive inhibitor of HIF-1α prolyl hydroxylase (HIF-PH).

For research use only. We do not sell to patients.

DMOG Chemical Structure

DMOG Chemical Structure

CAS No. : 89464-63-1

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 79 In-stock
Estimated Time of Arrival: December 31
50 mg USD 72 In-stock
Estimated Time of Arrival: December 31
100 mg USD 132 In-stock
Estimated Time of Arrival: December 31
200 mg USD 228 In-stock
Estimated Time of Arrival: December 31
500 mg USD 528 In-stock
Estimated Time of Arrival: December 31
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Customer Review

    DMOG purchased from MCE. Usage Cited in: Front Immunol. 2018 Jul 23;9:1667.

    Manipulation of the protein level of hypoxia-inducible factor 1 alpha (HIF-1α) in NR8383. 50 µM PX478 treatment for 20 h is used to downregulate the HIF-1α protein level in NR8383 cells (A). 1 mM DMOG treatment for 8 h is used to upregulate the HIF-1α protein level in NR8383 cells (B).

    DMOG purchased from MCE. Usage Cited in: Front Immunol. 2018 Jul 23;9:1667.

    The inhibitory effect of TAK-242 on the TLR4 mRNA and protein expression is detected by RT-qPCR and Western Blot in NR8383 cells.

    DMOG purchased from MCE. Usage Cited in: Cancer Biol Ther. 2016 Jun 2;17(6):625-34.

    HL-60 and Kasumi-1 cells are treated with different concentration of 2ME2 (0.1, 0.5, 2.5, or 5μM) for 24 hours or diluent control, and intracelluar protein levels are examined by Western blot.

    DMOG purchased from MCE. Usage Cited in: J Biol Chem. 2015 Jan 16;290(3):1389-94.

    The hypoxia induced the expression of NEK8. Cells are treated with CoCl2 (100 μM) or DMOG (100 μM) for 6 h or cultured for 12 h in hypoxia (1.0% oxygen). HIF-1α and NEK8 are analyzed by immunoblotting, GAPDH is employed as a loading control.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    DMOG (Dimethyloxallyl Glycine) is a cell-permeable and competitive inhibitor of HIF-1α prolyl hydroxylase (HIF-PH).

    IC50 & Target

    HIF-1α prolyl hydroxylase[1]

    In Vitro

    DMOG efficiently suppresses hydroxyproline synthesis in intact cells, but shows only weakly active in the microsomal system[1]. DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC[3].

    In Vivo

    DMOG inhibits endogenous HIF inactivation, and induces angiogenesis in ischaemic skeletal muscles of mice[2]. Up-regulation of hypoxia-inducible factor-1α by DMOG enhances the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats[4].

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 101.5 mg/mL (579.54 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.7097 mL 28.5486 mL 57.0972 mL
    5 mM 1.1419 mL 5.7097 mL 11.4194 mL
    10 mM 0.5710 mL 2.8549 mL 5.7097 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Cell Assay
    [3]

    To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [2]

    Two groups of mice (C57Bl6) are subjected to unilateral femoral artery ligation under fluothane inhalation anaesthesia (2% in O2). One group (n=11) receives dimethyloxalylglycine (DMOG) i.p. (8 mg in 0.5 mL saline) on days 1, 3, 5, 7 and 9 while the animals in the other group are injected with sterile saline (0.5 mL) at the same intervals (n=6). A third group is treated with DMOG without ligation (n=4) and four unoperated mice serve as controls. After 11 days mice are terminally anaesthetized and the extensor digitorum longus (EDL) and tibialis anterior (TA) muscles excised.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    175.14

    Formula

    C₆H₉NO₅

    CAS No.

    89464-63-1

    SMILES

    O=C(OC)CNC(C(OC)=O)=O

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Purity: 99.15%

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    Product Name:
    DMOG
    Cat. No.:
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    Cat. No.: HY-15893