2. Academic Validation
  3. Activation of long-non-coding RNA NEAT1 sponging microRNA-147 inhibits radiation damage by targeting PDPK1 in troxerutin radioprotection

Activation of long-non-coding RNA NEAT1 sponging microRNA-147 inhibits radiation damage by targeting PDPK1 in troxerutin radioprotection

  • iScience. 2023 Jan 5;26(2):105932. doi: 10.1016/j.isci.2023.105932.
Yong-Jian Hu 1 2,  Gui-Yuan Song 1 3 4,  Fan Zhang 1 2,  Nan Zhang 1,  Fei Wang 1,  Jing-Long Wang 1,  Xia Wang 5,  Tao-Yang Wang 1 2,  Yu-Feng Li 4,  Yi-di Yan 6,  Wen-Tao Dou 6,  Chen-Yi Cheng 6,  Ping Xu 1 2
Affiliations

Affiliations

  • 1 School of Food and Biomedicine, Zaozhuang University, Zaozhuang, Shandong 277160, China.
  • 2 Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, China.
  • 3 School of Public Health, Weifang Medical University, Weifang, Shandong 261000, China.
  • 4 Radiology Laboratory, Central Laboratory, Rizhao People's Hospital, Rizhao, Shandong 276800, China.
  • 5 College of Medical Laboratory, Xinxiang Medical University, Xinxiang, Henan 453003, China.
  • 6 Basic Medical School, Xinxiang Medical University, Xinxiang, Henan 453003, China.
PMID: 36698722 DOI: 10.1016/j.isci.2023.105932
Abstract

A better understanding of the molecular mechanism involving the lncRNA-miRNA-mRNA network underlying radiation damage can be beneficial for radioprotection. This study was designed to investigate the potential role of lncRNA NEAT1, miR-147 and Phosphoinositide Dependent Protein Kinase 1 (PDPK1) interaction in radioprotection by troxerutin (TRT). We first demonstrated that NEAT1 sponged miR-147, and PDPK1 mRNA was the primary target of miR-147. In the cells, the NEAT1 and PDPK1 levels were downregulated after the radiation but increased after the treatment with TRT. The miR-147 level was significantly induced by radiation and inhibited by TRT. NEAT1 negatively regulated the expression of miR-147, whereas miR-47 targeted PDPK1 to downregulate its expression. In radioprotection, TRT effectively upregulated NEAT1 to inhibit miR-147 and to upregulate PDPK1. We concluded that TRT could promote radioprotection by stimulating NEAT1 to upregulate PDPK1 expression by suppressing miR-147. NEAT1 could be a critical therapeutic target of radiation damage.

Keywords

Biological sciences; Cell biology; Molecular biology; Molecular mechanism of gene regulation.

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