2. Academic Validation
  3. An injectable liposome-anchored teriparatide incorporated gallic acid-grafted gelatin hydrogel for osteoarthritis treatment

An injectable liposome-anchored teriparatide incorporated gallic acid-grafted gelatin hydrogel for osteoarthritis treatment

  • Nat Commun. 2023 May 31;14(1):3159. doi: 10.1038/s41467-023-38597-0.
Guoqing Li # 1 2,  Su Liu # 1 2,  Yixiao Chen # 1 2,  Jin Zhao 1 2,  Huihui Xu 1 2,  Jian Weng 1 2,  Fei Yu 1 2,  Ao Xiong 1 2,  Anjaneyulu Udduttula 3,  Deli Wang 1 2,  Peng Liu 4 5,  Yingqi Chen 6 7,  Hui Zeng 8 9 10
Affiliations

Affiliations

  • 1 Department of Bone & Joint Surgery, Peking University Shenzhen Hospital, Shenzhen, 518036, PR China.
  • 2 National & Local Joint Engineering Research Center of Orthopaedic Biomaterials, Peking University Shenzhen Hospital, Shenzhen, 518036, PR China.
  • 3 Centre for Biomaterials, Cellular and Molecular Theranostics (CBCMT), Vellore Institute of Technology (VIT), Vellore, Tamil Nadu, 632014, India.
  • 4 Department of Bone & Joint Surgery, Peking University Shenzhen Hospital, Shenzhen, 518036, PR China. [email protected].
  • 5 National & Local Joint Engineering Research Center of Orthopaedic Biomaterials, Peking University Shenzhen Hospital, Shenzhen, 518036, PR China. [email protected].
  • 6 Department of Bone & Joint Surgery, Peking University Shenzhen Hospital, Shenzhen, 518036, PR China. [email protected].
  • 7 National & Local Joint Engineering Research Center of Orthopaedic Biomaterials, Peking University Shenzhen Hospital, Shenzhen, 518036, PR China. [email protected].
  • 8 Department of Bone & Joint Surgery, Peking University Shenzhen Hospital, Shenzhen, 518036, PR China. [email protected].
  • 9 National & Local Joint Engineering Research Center of Orthopaedic Biomaterials, Peking University Shenzhen Hospital, Shenzhen, 518036, PR China. [email protected].
  • 10 Department of Bone & Joint Surgery, Peking University Shenzhen Hospital, 1120 Lianhua Road, Futian District, Shenzhen, Guangdong Province, PR China. [email protected].
  • # Contributed equally.
PMID: 37258510 DOI: 10.1038/s41467-023-38597-0
Abstract

Intra-articular injection of therapeutics is an effective strategy for treating osteoarthritis (OA), but it is hindered by rapid drug diffusion, thereby necessitating high-frequency injections. Hence, the development of a biofunctional hydrogel for improved delivery is required. In this study, we introduce a liposome-anchored teriparatide (PTH (1-34)) incorporated into a gallic acid-grafted gelatin injectable hydrogel (GLP hydrogel). We show that the GLP hydrogel can form in situ and without affecting knee motion after intra-articular injection in mice. We demonstrate controlled, sustained release of PTH (1-34) from the GLP hydrogel. We find that the GLP hydrogel promotes ATDC5 cell proliferation and protects the IL-1β-induced ATDC5 cells from further OA progression by regulating the PI3K/Akt signaling pathway. Further, we show that intra-articular injection of hydrogels into an OA-induced mouse model promotes glycosaminoglycans synthesis and protects the cartilage from degradation, supporting the potential of this biomaterial for OA treatment.

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