H-89 dihydrochloride
Based on 182 publication(s) in Google Scholar
H-89 dihydrochloride is a potent and selective inhibitor of protein kinase A (PKA) with an IC50 of 48 nM and has weak inhibition on PKG, PKC, Casein Kinase.
For research use only. We do not sell to patients.
- Purity: 99.97%
- CAS No.: 130964-39-5
- Formula: C20H22BrCl2N3O2S
- Molecular Weight:519.28
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)
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Biological Activity
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Publications Citing Use of MedChemExpress (MCE) H-89 dihydrochloride
More- Signal Transduct Target Ther. 2023 Aug 9;8(1):290. [Abstract]
- Cell Metab. 2025 Jan 7;37(1):87-103.e10. [Abstract]
- Cell Metab. 2021 Oct 5;33(10):2021-2039.e8. [Abstract]
- Cell Metab. 2021 Mar 2;33(3):565-580.e7. [Abstract]
- Nat Immunol. 2024 Nov;25(11):2057-2067. [Abstract]
- Nat Metab. 2026 Mar;8(3):704-721. [Abstract]
- Nat Metab. 2024 Apr;6(4):708-723. [Abstract]
- Ann Rheum Dis. 2025 Oct 28:S0003-4967(25)04428-0. [Abstract]
- Cell Mol Immunol. 2023 Feb;20(2):175-188. [Abstract]
- Mol Cell. 2023 Apr 6;83(7):1093-1108.e8. [Abstract]
- ACS Nano. 2025 Jul 1;19(25):22755-22766. [Abstract]
- Nat Commun. 2025 Jul 1;16(1):5425. [Abstract]
- Nat Commun. 2025 Jul 28;16(1):6942. [Abstract]
- Nat Commun. 2023 May 31;14(1):3159. [Abstract]
- Nat Commun. 2022 Nov 4;13(1):6648. [Abstract]
- Acta Pharm Sin B. 2024 Mar;14(3):1222-1240. [Abstract]
- Autophagy. 2026 Mar 15:1-22. [Abstract]
- J Mater Sci Technol. 2023 Jun 30.
- Adv Sci (Weinh). 2025 Dec 22:e15246. [Abstract]
- Adv Sci (Weinh). 2025 Sep 26:e02395. [Abstract]
- Adv Sci (Weinh). 2025 Apr 28:e2409837. [Abstract]
- Adv Sci (Weinh). 2025 Feb 7:e2413324. [Abstract]
- Adv Sci (Weinh). 2024 Jul 1:e2403640. [Abstract]
- Adv Sci (Weinh). 2021 Dec;8(24):e2100808. [Abstract]
- Neuro Oncol. 2024 Jan 5;26(1):137-152. [Abstract]
- Theranostics. 2021 Mar 24;11(12):5650-5674. [Abstract]
- Theranostics. 2021 Jan 1;11(6):2612-2633. [Abstract]
- J Adv Res. 2025 Apr 22:S2090-1232(25)00278-4. [Abstract]
- Biomaterials. 2022 Dec:291:121902. [Abstract]
- J Exp Clin Cancer Res. 2024 Apr 4;43(1):105. [Abstract]
- J Exp Clin Cancer Res. 2021 Apr 8;40(1):122. [Abstract]
- Mater Today Bio. 2026 Mar 10:37:103021. [Abstract]
- J Neuroinflammation. 2025 Oct 14;22(1):233. [Abstract]
- Int J Biol Sci. 2024 Aug 19;20(11):4476-4495. [Abstract]
- Int J Biol Sci. 2023 Apr 2;19(7):2006-2019. [Abstract]
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- Cell Death Dis. 2025 Jan 20;16(1):31. [Abstract]
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- Genes Dis. 2020 Nov 21;8(6):867-881. [Abstract]
- NPJ Biofilms Microbiomes. 2023 Feb 8;9(1):8. [Abstract]
- Proc Natl Acad Sci U S A. 2026 May 12;123(19):e2536312123. [Abstract]
- Proc Natl Acad Sci U S A. 2026 Jul 7;123(27):e2523789123.
- Small Methods. 2025 Mar;9(3):e2401144. [Abstract]
- Cell Commun Signal. 2024 Jul 25;22(1):375. [Abstract]
- Cell Commun Signal. 2022 Apr 12;20(1):52. [Abstract]
- Cell Commun Signal. 2021 Oct 11;19(1):103. [Abstract]
- Int J Biol Macromol. 2026 Jan;340(Pt 1):150002. [Abstract]
- Int J Biol Macromol. 2025 Apr 26:143548. [Abstract]
- Phytomedicine. 2025 Dec:149:157494. [Abstract]
- Phytomedicine. 2025 Mar:138:156375. [Abstract]
- Free Radic Biol Med. 2024 May 31:222:72-84. [Abstract]
- Sci Total Environ. 2022 Oct 10:842:156854. [Abstract]
- Brain Behav Immun. 2026 Mar 6:135:106529. [Abstract]
- Brain Behav Immun. 2024 Jul:119:36-50. [Abstract]
- Brain Behav Immun. 2024 Jan:115:667-679. [Abstract]
- Anim Nutr. 2022 Dec 31:13:185-196. [Abstract]
- Biomed Pharmacother. 2022 Apr:148:112728. [Abstract]
- Chem Sci. 2019 Dec 6;11(4):1132-1139. [Abstract]
- Stem Cell Res Ther. 2020 May 19;11(1):182. [Abstract]
- Stem Cell Res Ther. 2020 Jan 3;11(1):2. [Abstract]
- Aging Cell. 2018 Jun;17(3):e12754. [Abstract]
- Cell Death Discov. 2025 Apr 6;11(1):148. [Abstract]
- Microbiol Res. 2024 May 24:285:127779. [Abstract]
- Cell Rep. 2022 Oct 18;41(3):111498. [Abstract]
- Cell Rep. 2021 Sep 21;36(12):109726. [Abstract]
- J Med Chem. 2025 Nov 13;68(21):23277-23299. [Abstract]
- Clin Transl Med. 2021 Jan;11(1):e266. [Abstract]
- Sci Signal. 2020 Nov 24;13(659):eaax0273. [Abstract]
- Elife. 2023 Jan 16:12:e81438. [Abstract]
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- Phytother Res. 2023 Apr;37(4):1330-1345. [Abstract]
- Transl Psychiatry. 2025 Oct 6;15(1):366. [Abstract]
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- Prog Neurobiol. 2021 Jul:202:102041. [Abstract]
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- J Bone Miner Res. 2021 Aug;36(8):1605-1620. [Abstract]
- Chin Med. 2026 Feb 3;21(1):62. [Abstract]
- Biochem Pharmacol. 2026 Jun:248:117840. [Abstract]
- Chem Biol Interact. 2024 Sep 2:111224. [Abstract]
- Nutr Diabetes. 2024 Jul 23;14(1):56. [Abstract]
- Cells. 2023 Apr 6;12(7):1098. [Abstract]
- Life Sci. 2026 Jan 15:385:124138. [Abstract]
- Glia. 2026 Feb;74(2):e70118. [Abstract]
- Life Sci. 2025 May 15:369:123553. [Abstract]
- Life Sci. 2022 Aug 15:303:120676. [Abstract]
- CNS Neurosci Ther. 2024 May;30(5):e14742. [Abstract]
- Nutrients. 2020 Jan 26;12(2):329. [Abstract]
- Int J Mol Sci. 2023 Jan 16;24(2):1771. [Abstract]
- Int J Mol Sci. 2022 Dec 24;24(1):320. [Abstract]
- Int J Mol Sci. 2022 Oct 20;23(20):12595. [Abstract]
- Int J Mol Sci. 2018 Oct 7;19(10). pii: E3053. [Abstract]
- Front Pharmacol. 2018 Apr 12:9:362. [Abstract]
- Invest Ophthalmol Vis Sci. 2025 Jul 1;66(9):68. [Abstract]
- Int Immunopharmacol. 2025 May 17:158:114885. [Abstract]
- Eur J Pharmacol. 2024 Nov 6:985:177106. [Abstract]
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- BMC Biol. 2024 May 3;22(1):104. [Abstract]
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- Mol Neurobiol. 2025 Jan;62(1):366-385. [Abstract]
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- J Cell Mol Med. 2024 Apr;28(8):e18303. [Abstract]
- FASEB J. 2020 Oct;34(10):13376-13395. [Abstract]
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- J Lipid Res. 2026 Feb;67(2):100981. [Abstract]
- iScience. 2025 Jan 21;28(2):111856. [Abstract]
- iScience. 2021 Jul 8;24(8):102820. [Abstract]
- J Funct Foods. 2025 Aug.
- J Funct Foods. 2023 Dec, 111, 105902.
- J Neurosci. 2016 Oct 12;36(41):10560-10573. [Abstract]
- Sci Rep. 2025 Oct 16;15(1):36270. [Abstract]
- J Biol Chem. 2021 Jan-Jun:296:100644. [Abstract]
- Aging (Albany NY). 2020 Jun 9;12(11):10951-10968. [Abstract]
- Aging (Albany NY). 2018 Jul 24;10(7):1722-1744. [Abstract]
- J Periodontol. 2019 Sep;90(9):1002-1013. [Abstract]
- Aquac Rep. 2024 Jun, 36, 102110.
- BMC Genomics. 2024 Apr 3;25(1):337. [Abstract]
- Heliyon. 2024 Sep 19;10(19):e37958. [Abstract]
- Biol Trace Elem Res. 2021 Jul;199(7):2687-2694. [Abstract]
- Psychoneuroendocrinology. 2020 Jul;117:104680. [Abstract]
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- J Gastroenterol Hepatol. 2025 Jul 31. [Abstract]
- Andrology. 2025 Jul 2. [Abstract]
- Acta Biochim Biophys Sin (Shanghai). 2023 Nov 15;55(12):1855-1863. [Abstract]
- Front Physiol. 2022 Jan 5:12:736920. [Abstract]
- Front Physiol. 2021 Oct 28;12:770430. [Abstract]
- Endocrinology. 2023 Mar 13;164(5):bqad042. [Abstract]
- Front Oncol. 2021 Oct 6;11:738300. [Abstract]
- Korean J Pain. 2023 Jul 1;36(3):335-346. [Abstract]
- Biol Reprod. 2025 Sep 18:ioaf213. [Abstract]
- Mol Reprod Dev. 2022 May;89(5-6):256-268. [Abstract]
- Arch Biochem Biophys. 2020 Jul 15;687:108375. [Abstract]
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- Toxicol Lett. 2022 Apr 15:359:84-95. [Abstract]
- Mol Brain. 2021 Jul 19;14(1):116. [Abstract]
- Neurogastroenterol Motil. 2020 Oct;32(10):1514-1528. [Abstract]
- Int J Biochem Cell Biol. 2026 Jun-Jul:195-196:106941. [Abstract]
- J Orthop Surg Res. 2023 Nov 8;18(1):849. [Abstract]
- Infect Immun. 2018 Dec 19;87(1). pii: e00508-1 [Abstract]
- BMC Pharmacol Toxicol. 2023 Nov 20;24(1):63. [Abstract]
- Vet Microbiol. 2021 May:256:109061. [Abstract]
- Fitoterapia. 2022 Nov:163:105336. [Abstract]
- Reprod Sci. 2023 Apr;30(4):1281-1290. [Abstract]
- Eur J Neurosci. 2025 Jul;62(2):e70198. [Abstract]
- PeerJ. 2022 Oct 25:10:e14192. [Abstract]
- Purinergic Signal. 2022 Sep;18(3):317-343. [Abstract]
- Exp Ther Med. 2022 Apr;23(4):248. [Abstract]
- Biochem Biophys Res Commun. 2024 Apr 9:703:149689. [Abstract]
- J Bone Miner Metab. 2021 Sep;39(5):748-756. [Abstract]
- Eur J Histochem. 2026 Jan 26;70(1). [Abstract]
- J Neurophysiol. 2024 Jan 1;131(1):64-74. [Abstract]
- Aquac Res. 2020;00:1-10.
- Comp Biochem Physiol B Biochem Mol Biol. 2026 Jun-Jul:284:111225. [Abstract]
- Gen Comp Endocrinol. 2026 Mar:379:114913. [Abstract]
- Biol Pharm Bull. 2024;47(6):1113-1118. [Abstract]
- Proteome Sci. 2023 Oct 11;21(1):16. [Abstract]
- J Int Med Res. 2021 Mar;49(3):300060521999527. [Abstract]
- Heart Surg Forum. 2024 Mar 17.
- bioRxiv. 2026 Feb 10.
- Res Sq. 2026 Jan 13.
- bioRxiv. 2025 Nov 28.
- AUTHOREA. 2025 May 27.
- bioRxiv. 2025 May 12.
- J Appl Biol Chem. 2025;68:26, 204‒211.
- Res Sq. 2025 Apr 08.
- SSRN. 2025 Apr 10.
- SSRN. 2025 Mar 24.
- Research Square Preprint. 2024 Dec 04.
- bioRxiv. 2024 Oct 22:2024.10.18.619152. [Abstract]
- bioRxiv. 2024 Oct 2:2024.09.30.615856. [Abstract]
- Res Sq. 2024 Jul 15.
- Res Sq. 2024 May 23.
- bioRxiv. 2024 Feb 8.
- Research Square Preprint. 2023 Sep 13.
- Evid Based Complement Alternat Med. 2020 Dec 15:2020:8514926. [Abstract]
- Research Square Preprint. 2020 Oct.
- IJMPNP. 2019,5(1): 8-15.
Customer Validation & Images
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RT-PCR
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WB
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Cell Proliferation/Viability Assay
Biological Activity
IC50: 48 nM (protein kinase A)
H-89 inhibits protein kinase A, in competitive fashion against ATP. H-89 causes a dose-dependent inhibition of the forskolin-induced protein phosphorylation, with no decrease in intracellular cyclic AMP levels in PC12D cells. H-89 significantly inhibits the forskolin-induced neurite outgrowth from PC12D cells. H-89 (30 μM) inhibits significantly cAMP-dependent histone IIb phosphorylation activity in PC12D cell lysates[1]. H-89 (1-2 μM) significantly slows the repriming rate in rat skinned fibres, most likely due to it deleteriously affecting the T-system potential. H-89 (10-100 μM) inhibits net Ca2+ uptake by the SR and affectes the Ca2+-sensitivity of the contractile apparatus in rat skinned fibres[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 130964-39-5
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Appearance Solid
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Molecular Weight 519.28
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Formula C20H22BrCl2N3O2S
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Color White to light yellow
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SMILES
O=S(C1=CC=CC2=C1C=CN=C2)(NCCNC/C=C/C3=CC=C(Br)C=C3)=O.Cl.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)
Publications (182)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
NPRC deletion mitigated atherosclerosis by inhibiting oxidative stress, inflammation and apoptosis in ApoE knockout mice. [Abstract]2023 Aug 9;8(1):290. PMID: 37553374 -
Cell Metab
Stress triggers irritable bowel syndrome with diarrhea through a spermidine-mediated decline in type I interferon. [Abstract]2025 Jan 7;37(1):87-103.e10. PMID: 39366386 -
Cell Metab
The thermogenic activity of adjacent adipocytes fuels the progression of ccRCC and compromises anti-tumor therapeutic efficacy. [Abstract]2021 Oct 5;33(10):2021-2039.e8. PMID: 34508696 -
Cell Metab
The phytochemical hyperforin triggers thermogenesis in adipose tissue via a Dlat-AMPK signaling axis to curb obesity. [Abstract]2021 Mar 2;33(3):565-580.e7. PMID: 33657393 -
Nat Immunol
2024 Nov;25(11):2057-2067. PMID: 39358444 -
Nat Metab
SLC6A6 imports taurine into mitochondria to sustain mitochondrial translation and tumour growth. [Abstract]2026 Mar;8(3):704-721. PMID: 41652173 -
Nat Metab
2024 Apr;6(4):708-723. PMID: 38499763 -
Ann Rheum Dis
2025 Oct 28:S0003-4967(25)04428-0. PMID: 41162285 -
Cell Mol Immunol
β-adrenoreceptor-triggered PKA activation negatively regulates the innate antiviral response. [Abstract]2023 Feb;20(2):175-188. PMID: 36600052
H-89 dihydrochloride purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2023 Feb;20(2):175-188. [Abstract]
H89 (5 μM; 6 h) reverses ISO (Isoprenaline hydrochloride)-triggered decreases in the mRNA levels of the IFNB1 gene, the expression of which has been induced by SeV infection, in THP1 cells.
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Mol Cell
Histone phosphorylation integrates the hepatic glucagon-PKA-CREB gluconeogenesis program in response to fasting. [Abstract]2023 Apr 6;83(7):1093-1108.e8. PMID: 36863348 -
ACS Nano
Reduced Artifacts in Nanoscale Ratiometric Imaging and Sensing via Point-Spread Function Matching. [Abstract]2025 Jul 1;19(25):22755-22766. PMID: 40539535 -
Nat Commun
Protein kinase A is a dependent factor and therapeutic target in mouse models of fibrous dysplasia. [Abstract]2025 Jul 1;16(1):5425. PMID: 40593719 -
Nat Commun
2025 Jul 28;16(1):6942. PMID: 40721606 -
Nat Commun
An injectable liposome-anchored teriparatide incorporated gallic acid-grafted gelatin hydrogel for osteoarthritis treatment. [Abstract]2023 May 31;14(1):3159. PMID: 37258510 -
Nat Commun
2022 Nov 4;13(1):6648. PMID: 36333322 -
Acta Pharm Sin B
GRK2 mediated degradation of SAV1 initiates hyperplasia of fibroblast-like synoviocytes in rheumatoid arthritis. [Abstract]2024 Mar;14(3):1222-1240. PMID: 38486990 -
Autophagy
Impaired mitophagy contributes to osteogenesis and mineralization disorders in fibrous dysplasia. [Abstract]2026 Mar 15:1-22. PMID: 41803635 -
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Adv Sci (Weinh)
Osmotic Tension Asymmetry Drives Electrotactic Migration via PDLIM7-Polarized Microfilament Coordination in Breast Cancer Cells. [Abstract]2025 Dec 22:e15246. PMID: 41431193 -
Adv Sci (Weinh)
Calhm6 Governs Macrophage Polarization Through Chp1-Camk4-Creb1 Axis and Ectosomal Delivery in Inflammatory Responses. [Abstract]2025 Sep 26:e02395. PMID: 40999918 -
Adv Sci (Weinh)
Microbiota-Derived Inosine Suppresses Systemic Autoimmunity via Restriction of B Cell Differentiation and Migration. [Abstract]2025 Apr 28:e2409837. PMID: 40289872 -
Adv Sci (Weinh)
Targeting the E Prostanoid Receptor EP4 Mitigates Cardiac Fibrosis Induced by β-Adrenergic Activation. [Abstract]2025 Feb 7:e2413324. PMID: 39921269 -
Adv Sci (Weinh)
Granulosa Cell-Layer Stiffening Prevents Escape of Mural Granulosa Cells from the Post-Ovulatory Follicle. [Abstract]2024 Jul 1:e2403640. PMID: 38946588 -
Adv Sci (Weinh)
2021 Dec;8(24):e2100808. PMID: 34719888 -
Neuro Oncol
A disintegrin and metalloproteinase 22 activates integrin β1 through its disintegrin domain to promote the progression of pituitary adenoma. [Abstract]2024 Jan 5;26(1):137-152. PMID: 37555799 -
Theranostics
Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. [Abstract]2021 Mar 24;11(12):5650-5674. PMID: 33897873 -
Theranostics
CXCL12-mediated HOXB5 overexpression facilitates Colorectal Cancer metastasis through transactivating CXCR4 and ITGB3. [Abstract]2021 Jan 1;11(6):2612-2633. PMID: 33456563 -
J Adv Res
A novel TGR5 agonist Sauchinone ameliorates IMQ induced murine psoriasis by regulating macrophage polarization. [Abstract]2025 Apr 22:S2090-1232(25)00278-4. PMID: 40274226 -
Biomaterials
Self-amplified ROS production from fatty acid oxidation enhanced tumor immunotherapy by atorvastatin/PD-L1 siRNA lipopeptide nanoplexes. [Abstract]2022 Dec:291:121902. PMID: 36371945 -
J Exp Clin Cancer Res
GPR65 sensing tumor-derived lactate induces HMGB1 release from TAM via the cAMP/PKA/CREB pathway to promote glioma progression. [Abstract]2024 Apr 4;43(1):105. PMID: 38576043 -
J Exp Clin Cancer Res
Efficacy and mechanism of the combination of PARP and CDK4/6 inhibitors in the treatment of triple-negative breast cancer. [Abstract]2021 Apr 8;40(1):122. PMID: 33832512 -
Mater Today Bio
PKA activation rescues myocardial injury elicited by silica nanoparticles through improving oxidative stress, mitochondrial health, and copper homeostasis. [Abstract]2026 Mar 10:37:103021. PMID: 41890951 -
J Neuroinflammation
IL-1β+ tumor-associated macrophages accelerate glioblastoma progression by amplifying the PGE2-EP4 signaling. [Abstract]2025 Oct 14;22(1):233. PMID: 41088217 -
Int J Biol Sci
Chronic Stress-induced Serotonin Impairs Intestinal Epithelial Cell Mitochondrial Biogenesis via the AMPK-PGC-1α Axis. [Abstract]2024 Aug 19;20(11):4476-4495. PMID: 39247815 -
Int J Biol Sci
Chronic stress promotes colorectal cancer progression by enhancing glycolysis through β2-AR/CREB1 signal pathway. [Abstract]2023 Apr 2;19(7):2006-2019. PMID: 37151872
H-89 dihydrochloride purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2023 Apr 2;19(7):2006-2019. [Abstract]
H-89 dihydrochloride (H-89; 10 μM; 2 h) blocks the epinephrine-induced phosphorylation of CREB1 in MC38 and HT-29 cells.
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Cell Death Dis
Activation of AKT via a dual mechanism enhances the susceptibility of melanoma cells to glucose deprivation. [Abstract]2025 Aug 7;16(1):595. PMID: 40774947 -
Cell Death Dis
S1PR3-driven positive feedback loop sustains STAT3 activation and keratinocyte hyperproliferation in psoriasis. [Abstract]2025 Jan 20;16(1):31. PMID: 39833165 -
Cell Death Dis
2024 Sep 30;15(9):699. PMID: 39349432 -
Genes Dis
2020 Nov 21;8(6):867-881. PMID: 34522714 -
NPJ Biofilms Microbiomes
Gut microbiota-mediated secondary bile acid alleviates Staphylococcus aureus-induced mastitis through the TGR5-cAMP-PKA-NF-κB/NLRP3 pathways in mice. [Abstract]2023 Feb 8;9(1):8. PMID: 36755021 -
Proc Natl Acad Sci U S A
A PKA-selective inhibitor captures an open but more ordered conformation of the PKA catalytic subunit. [Abstract]2026 May 12;123(19):e2536312123. PMID: 42096309 -
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Small Methods
An Integrated Microcurrent Delivery System Facilitates Human Parathyroid Hormone Delivery for Enhancing Osteoanabolic Effect. [Abstract]2025 Mar;9(3):e2401144. PMID: 39420694 -
Cell Commun Signal
Olanzapine suppresses mPFC activity-norepinephrine releasing to alleviate CLOCK-enhanced cancer stemness under chronic stress. [Abstract]2024 Jul 25;22(1):375. PMID: 39054537 -
Cell Commun Signal
Receptor activity-modifying protein 1 regulates mouse skin fibroblast proliferation via the Gαi3-PKA-CREB-YAP axis. [Abstract]2022 Apr 12;20(1):52. PMID: 35413847 -
Cell Commun Signal
Zinc-mediated activation of CREB pathway in proliferation of pulmonary artery smooth muscle cells in pulmonary hypertension. [Abstract]2021 Oct 11;19(1):103. PMID: 34635097 -
Int J Biol Macromol
Effect and mechanism of maternal prolactin levels on depressive-like behavior in prenatally stressed offspring. [Abstract]2026 Jan;340(Pt 1):150002. PMID: 41485664 -
Int J Biol Macromol
Genome-wide identification and expression analysis of neuropeptide G protein-coupled receptors in Urechis unicinctus (Annelida, Echiura). [Abstract]2025 Apr 26:143548. PMID: 40294673 -
Phytomedicine
Z-Ligustilide's antidepressant effects in adolescent depression involve the PTEN-induced putative kinase 1 (PINK1) - protein kinase A (PKA)- Na+/Ca2+ exchanger (NCLX) pathway, facilitating mitochondrial endosymbiosis and promoting mitophagy in microglia. [Abstract]2025 Dec:149:157494. PMID: 41218379 -
Phytomedicine
Shouhui Tongbian Capsule ameliorates obesity by enhancing energy consumption and promoting lipolysis via cAMP-PKA pathway. [Abstract]2025 Mar:138:156375. PMID: 39848021 -
Free Radic Biol Med
METTL3 deficiency leads to ovarian insufficiency due to IL-1β overexpression in theca cells. [Abstract]2024 May 31:222:72-84. PMID: 38825211 -
Sci Total Environ
Silica nanoparticles perturbed mitochondrial dynamics and induced myocardial apoptosis via PKA-DRP1-mitochondrial fission signaling. [Abstract]2022 Oct 10:842:156854. PMID: 35750168 -
Brain Behav Immun
NR2B-CaMKII signaling in the dentate gyrus driven by astrocytic P2Y1Rs mediates the antidepressant effect of low-dose LPS. [Abstract]2026 Mar 6:135:106529. PMID: 41796645 -
Brain Behav Immun
Dexamethasone attenuates neuropathic pain through spinal microglial expression of dynorphin A via the cAMP/PKA/p38 MAPK/CREB signaling pathway. [Abstract]2024 Jul:119:36-50. PMID: 38555991 -
Brain Behav Immun
Gut microbiota-mediated ursodeoxycholic acids regulate the inflammation of microglia through TGR5 signaling after MCAO. [Abstract]2024 Jan:115:667-679. PMID: 37989444 -
Anim Nutr
DHA induces adipocyte lipolysis through endoplasmic reticulum stress and the cAMP/PKA signaling pathway in grass carp (Ctenopharyngodon idella). [Abstract]2022 Dec 31:13:185-196. PMID: 37123617 -
Biomed Pharmacother
Teriparatide induces angiogenesis in ischemic cerebral infarction zones of rats through AC/PKA signaling and reduces ischemia-reperfusion injury. [Abstract]2022 Apr:148:112728. PMID: 35220030 -
Chem Sci
2019 Dec 6;11(4):1132-1139. PMID: 34084370 -
Stem Cell Res Ther
Mesenchymal stem cells combined with liraglutide relieve acute lung injury through apoptotic signaling restrained by PKA/β-catenin. [Abstract]2020 May 19;11(1):182. PMID: 32429994 -
Stem Cell Res Ther
Effect of hCMSCs and liraglutide combination in ALI through cAMP/PKAc/β-catenin signaling pathway. [Abstract]2020 Jan 3;11(1):2. PMID: 31900217 -
Aging Cell
nNOS-CAPON interaction mediates amyloid-β-induced neurotoxicity, especially in the early stages. [Abstract]2018 Jun;17(3):e12754. PMID: 29577585 -
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J Med Chem
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Clin Transl Med
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Sci Signal
Airway relaxation mechanisms and structural basis of osthole for improving lung function in asthma. [Abstract]2020 Nov 24;13(659):eaax0273. PMID: 33234690 -
Elife
2023 Jan 16:12:e81438. PMID: 36645741 -
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Effects on Synaptic Plasticity Markers in Fetal Mice and HT22 Neurons upon F-53B Exposure: The Role of PKA Cytoplasmic Retention. [Abstract]2024 Aug 16;2(11):776-785. PMID: 39568694 -
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Protective effects of 4-geranyloxy-2,6-dihydroxybenzophenonel on DSS-induced ulcerative colitis in mice via regulation of cAMP/PKA/CREB and NF-κB signaling pathways. [Abstract]2023 Apr;37(4):1330-1345. PMID: 36428266 -
Transl Psychiatry
TGR5 dysfunction underlies chronic social defeat stress via cAMP/PKA signaling pathway in the hippocampus. [Abstract]2025 Oct 6;15(1):366. PMID: 41053031 -
J Agric Food Chem
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Prog Neurobiol
2021 Jul:202:102041. PMID: 33766679 -
Ecotoxicol Environ Saf
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Cancer Cell Int
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Nutrients
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Int J Mol Sci
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Front Pharmacol
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Am J Physiol Cell Physiol
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Poult Sci
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J Lipid Res
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iScience
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iScience
Pleiotropic roles of Ras GTPases in the nematode-trapping fungus Arthrobotrys oligospora identified through multi-omics analyses. [Abstract]2021 Jul 8;24(8):102820. PMID: 34337364 -
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J Neurosci
Olig2-Targeted G-Protein-Coupled Receptor Gpr17 Regulates Oligodendrocyte Survival in Response to Lysolecithin-Induced Demyelination. [Abstract]2016 Oct 12;36(41):10560-10573. PMID: 27733608
H-89 dihydrochloride purchased from MedChemExpress. Usage Cited in: J Neurosci. 2016 Oct 12;36(41):10560-10573. [Abstract]
The inhibition of PKA signaling with PKA inhibitor H89 reduces cell viability observed after Pranlukast treatment.
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Sci Rep
2025 Oct 16;15(1):36270. PMID: 41102391 -
J Biol Chem
HIV-1 exposure promotes PKG1-mediated phosphorylation and degradation of stathmin to increase epithelial barrier permeability. [Abstract]2021 Jan-Jun:296:100644. PMID: 33839152 -
Aging (Albany NY)
Fibroblast growth factor 2 contributes to the effect of salidroside on dendritic and synaptic plasticity after cerebral ischemia/reperfusion injury. [Abstract]2020 Jun 9;12(11):10951-10968. PMID: 32518214 -
Aging (Albany NY)
Heme oxygenase-1 ameliorates oxidative stress-induced endothelial senescence via regulating endothelial nitric oxide synthase activation and coupling. [Abstract]2018 Jul 24;10(7):1722-1744. PMID: 30048241
H-89 dihydrochloride purchased from MedChemExpress. Usage Cited in: Aging (Albany NY). 2018 Jul 24;10(7):1722-1744. [Abstract]
Expressions of p-eNOS (Ser1177) and total eNOS are measured in HUVECs treated with or without H2O2 and Hemin in the presence or absence of Akt inhibitor MK-2206, PKA inhibitor H-89 or AMPK inhibitor Compound C (CP-C).
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J Periodontol
Intermittent parathyroid hormone promotes cementogenesis in a PKA- and ERK1/2-dependent manner. [Abstract]2019 Sep;90(9):1002-1013. PMID: 31026057 -
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BMC Genomics
Identification of the principal neuropeptide MIP and its action pathway in larval settlement of the echiuran worm Urechis unicinctus. [Abstract]2024 Apr 3;25(1):337. PMID: 38641568 -
Heliyon
CD38 symmetric dimethyl site R58 promotes malignant tumor cell immune escape by regulating the cAMP-GSK3β-PD-L1 axis. [Abstract]2024 Sep 19;10(19):e37958. PMID: 39386836 -
Biol Trace Elem Res
2021 Jul;199(7):2687-2694. PMID: 32926327 -
Psychoneuroendocrinology
2020 Jul;117:104680. PMID: 32387876 -
Bone
hPTH(3-34)(29-34) selectively activated PKC and mimicked osteoanabolic effects of hPTH(1-34). [Abstract]2020 Jun;135:115326. PMID: 32200023 -
Exp Cell Res
Down-regulation of A3AR signaling by IL-6-induced GRK2 activation contributes to Th17 cell differentiation. [Abstract]2021 Feb 15;399(2):112482. PMID: 33434531 -
J Gastroenterol Hepatol
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Andrology
Ubiquitin-mediated immunoglobulin-binding protein 1 degradation promotes autophagy and sperm capacitation in vitro. [Abstract]2025 Jul 2. PMID: 40600667 -
Acta Biochim Biophys Sin (Shanghai)
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Front Physiol
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Front Physiol
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Endocrinology
2023 Mar 13;164(5):bqad042. PMID: 36929849 -
Front Oncol
SUZ12 Loss Amplifies the Ras/ERK Pathway by Activating Adenylate Cyclase 1 in NF1-Associated Neurofibromas. [Abstract]2021 Oct 6;11:738300. PMID: 34692515 -
Korean J Pain
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Biol Reprod
Activation of cAMP/PKA and ERK signaling pathways and potential role of Phoenixin in the reproductive axis of spotted scat (Scatophagus argus)†. [Abstract]2025 Sep 18:ioaf213. PMID: 40973150 -
Mol Reprod Dev
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Arch Biochem Biophys
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Peptides
2026 Jan:195:171463. PMID: 41485715 -
Peptides
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Mol Brain
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Neurogastroenterol Motil
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Int J Biochem Cell Biol
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J Orthop Surg Res
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Infect Immun
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H-89 dihydrochloride purchased from MedChemExpress. Usage Cited in: Infect Immun. 2018 Dec 19;87(1). pii: e00508-1 [Abstract]
J774A.1 cells are pre-treated with 473 U0126 (10 μM), SB208530 (10 μM), SP600125 (10 μM), or H89 (50 μM), then infected with ΔeseH E. piscicida at the MOI of 10 for 3.5 h. Cell lysates were probed with anti-phospho-p65 (Ser276), anti-p65, and anti-β-actin antibodies.
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Eur J Neurosci
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PeerJ
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bioRxiv
Rapid Hippocampal Synaptic Potentiation Induced by Ketamine Metabolite ( 2R , 6R )-Hydroxynorketamine Persistently Primes Synaptic Plasticity. [Abstract]2024 Oct 22:2024.10.18.619152. PMID: 39484512 -
bioRxiv
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Evid Based Complement Alternat Med
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Solvent & Solubility
DMSO : 100 mg/mL (192.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 5 mg/mL (9.63 mM; ultrasonic and warming and heat to 80°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% Saline
Solubility: 5 mg/mL (9.63 mM); Suspended solution; Need ultrasonic
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 1% DMSO 99% Saline
Solubility: ≥ 0.55 mg/mL (1.06 mM); Clear solution
Please enter the basic information of animal experiments:
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-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
After 48 h in culture, PCl2D cells are cultured in test medium containing 30 μM H-89 for 1 h and then exposed to fresh medium that contained both 10 μM forskolin and 30 μM H-89. Cells are scraped off with a rubber policeman and sonicated in the presence of 0.5 mL of 6% trichloroacetic acid. To extract trichloroacetic acid, 2 mL of petroleum ether is added, the preparation mixed and centrifuged at 3000 rpm for 10 min. After aspiration of the upper layer, the residue sample solution is used for determination.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Male albino mice weighing 20-25 g are obtained. BL-191 (25, 50, 100 mg/kg), bucladesine (50, 100, 300 nM/mouse) and H-89 (0.05, 0.1, 0.2 mg/100 g) are administered intraperitoneally (i.p.) 30 min before intravenous (i.v.) infusion of PTZ. In combination groups, the first and second components are injected 45 and 30 min before PTZ infusion. In all groups, the respective control animalsreceive an appropriate volume of vehicle. For the i.v. infusion, the needle is inserted into the lateral tail vein, fixed to the tail vein by a narrow piece of adhesive tape, and the animal is allowed to move freely. PTZ solution is infused at a concentration rate of 1 mL/min.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Chijiwa T, et al. Inhibition of forskolin-induced neurite outgrowth and protein phosphorylation by a newly synthesized selective inhibitor of cyclic AMP-dependent protein kinase, N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H-89), of PC12D pheochromocytoma cells. J Biol Chem. 1990 Mar 25;265(9):5267-72. [Content Brief]
[2]. Blazev R, et al. Effects of the PKA inhibitor H-89 on excitation-contraction coupling in skinned and intact skeletal muscle fibres. J Muscle Res Cell Motil. 2001;22(3):277-86. [Content Brief]
[3]. Hosseini-Zare MS, et al. Effects of BL-191 and H-89 on epileptogenic activity of bucladesine in pentylenetetrazol-treated mice. Eur J Pharmacol. 2011 Nov 30;670(2-3):464-70. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.9257 mL | 9.6287 mL | 19.2574 mL | 48.1436 mL |
| 5 mM | 0.3851 mL | 1.9257 mL | 3.8515 mL | 9.6287 mL | |
| DMSO | 10 mM | 0.1926 mL | 0.9629 mL | 1.9257 mL | 4.8144 mL |
| 15 mM | 0.1284 mL | 0.6419 mL | 1.2838 mL | 3.2096 mL | |
| 20 mM | 0.0963 mL | 0.4814 mL | 0.9629 mL | 2.4072 mL | |
| 25 mM | 0.0770 mL | 0.3851 mL | 0.7703 mL | 1.9257 mL | |
| 30 mM | 0.0642 mL | 0.3210 mL | 0.6419 mL | 1.6048 mL | |
| 40 mM | 0.0481 mL | 0.2407 mL | 0.4814 mL | 1.2036 mL | |
| 50 mM | 0.0385 mL | 0.1926 mL | 0.3851 mL | 0.9629 mL | |
| 60 mM | 0.0321 mL | 0.1605 mL | 0.3210 mL | 0.8024 mL | |
| 80 mM | 0.0241 mL | 0.1204 mL | 0.2407 mL | 0.6018 mL | |
| 100 mM | 0.0193 mL | 0.0963 mL | 0.1926 mL | 0.4814 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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