H-89 dihydrochloride
Based on 214 publication(s) in Google Scholar
H-89 dihydrochloride is a potent and selective inhibitor of protein kinase A (PKA) with an IC50 of 48 nM and has weak inhibition on PKG, PKC, Casein Kinase.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 99.12%
- No. CAS: 130964-39-5
- Fòrmula: C20H22BrCl2N3O2S
- Peso molecular:519.28
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Almacenamiento:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)
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Chemical Information
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Publications Citing Use of MedChemExpress (MCE) H-89 dihydrochloride
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- Cell Metab. 2021 Oct 5;33(10):2021-2039.e8. [Abstract]
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- Nat Immunol. 2024 Nov;25(11):2057-2067. [Abstract]
- Ann Rheum Dis. 2025 Oct 28:S0003-4967(25)04428-0. [Abstract]
- Cell Mol Immunol. 2023 Feb;20(2):175-188. [Abstract]
- Autophagy. 2026 Apr 23:1-26. [Abstract]
- Autophagy. 2026 Mar 15:1-22. [Abstract]
- Nat Commun. 2025 Jul 1;16(1):5425. [Abstract]
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Customer Validation & Images
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RT-PCR
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WB
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WB
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WB
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Cell Proliferation/Viability Assay
Actividad biológica
Descripciòn
IC50 & Target
IC50: 48 nM (protein kinase A)
In Vitro
H-89 inhibits protein kinase A, in competitive fashion against ATP. H-89 causes a dose-dependent inhibition of the forskolin-induced protein phosphorylation, with no decrease in intracellular cyclic AMP levels in PC12D cells. H-89 significantly inhibits the forskolin-induced neurite outgrowth from PC12D cells. H-89 (30 μM) inhibits significantly cAMP-dependent histone IIb phosphorylation activity in PC12D cell lysates[1]. H-89 (1-2 μM) significantly slows the repriming rate in rat skinned fibres, most likely due to it deleteriously affecting the T-system potential. H-89 (10-100 μM) inhibits net Ca2+ uptake by the SR and affectes the Ca2+-sensitivity of the contractile apparatus in rat skinned fibres[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 130964-39-5
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Appearance Solid
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Peso molecular 519.28
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Fòrmula C20H22BrCl2N3O2S
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Color White to light yellow
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SMILES
O=S(C1=CC=CC2=C1C=CN=C2)(NCCNC/C=C/C3=CC=C(Br)C=C3)=O.Cl.Cl
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, sealed storage, away from moisture
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)
Publications (214)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
NPRC deletion mitigated atherosclerosis by inhibiting oxidative stress, inflammation and apoptosis in ApoE knockout mice. [Abstract]2023 Aug 9;8(1):290. PMID: 37553374 -
Cell Metab
Stress triggers irritable bowel syndrome with diarrhea through a spermidine-mediated decline in type I interferon. [Abstract]2025 Jan 7;37(1):87-103.e10. PMID: 39366386 -
Cell Metab
The thermogenic activity of adjacent adipocytes fuels the progression of ccRCC and compromises anti-tumor therapeutic efficacy. [Abstract]2021 Oct 5;33(10):2021-2039.e8. PMID: 34508696 -
Cell Metab
The phytochemical hyperforin triggers thermogenesis in adipose tissue via a Dlat-AMPK signaling axis to curb obesity. [Abstract]2021 Mar 2;33(3):565-580.e7. PMID: 33657393 -
Nat Metab
SLC6A6 imports taurine into mitochondria to sustain mitochondrial translation and tumour growth. [Abstract]2026 Mar;8(3):704-721. PMID: 41652173 -
Nat Metab
2024 Apr;6(4):708-723. PMID: 38499763 -
Nat Immunol
2024 Nov;25(11):2057-2067. PMID: 39358444 -
Ann Rheum Dis
2025 Oct 28:S0003-4967(25)04428-0. PMID: 41162285 -
Cell Mol Immunol
β-adrenoreceptor-triggered PKA activation negatively regulates the innate antiviral response. [Abstract]2023 Feb;20(2):175-188. PMID: 36600052
H-89 dihydrochloride purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2023 Feb;20(2):175-188. [Abstract]
H89 (5 μM; 6 h) reverses ISO (Isoprenaline hydrochloride)-triggered decreases in the mRNA levels of the IFNB1 gene, the expression of which has been induced by SeV infection, in THP1 cells.
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Autophagy
PBK-Loaded secretory autophagosomes drive radiotherapy-induced systemic adipose depletion via MAPK/ERK-PRKA/PKA-LIPE/HSL signaling: a therapeutic target for esophageal cancer cachexia. [Abstract]2026 Apr 23:1-26. PMID: 41988986 -
Autophagy
Impaired mitophagy contributes to osteogenesis and mineralization disorders in fibrous dysplasia. [Abstract]2026 Mar 15:1-22. PMID: 41803635 -
Nat Commun
Protein kinase A is a dependent factor and therapeutic target in mouse models of fibrous dysplasia. [Abstract]2025 Jul 1;16(1):5425. PMID: 40593719 -
Nat Commun
2025 Jul 28;16(1):6942. PMID: 40721606 -
Nat Commun
An injectable liposome-anchored teriparatide incorporated gallic acid-grafted gelatin hydrogel for osteoarthritis treatment. [Abstract]2023 May 31;14(1):3159. PMID: 37258510 -
Nat Commun
2022 Nov 4;13(1):6648. PMID: 36333322 -
ACS Nano
Reduced Artifacts in Nanoscale Ratiometric Imaging and Sensing via Point-Spread Function Matching. [Abstract]2025 Jul 1;19(25):22755-22766. PMID: 40539535 -
J Adv Res
A novel TGR5 agonist Sauchinone ameliorates IMQ induced murine psoriasis by regulating macrophage polarization. [Abstract]2025 Apr 22:S2090-1232(25)00278-4. PMID: 40274226 -
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Sci Immunol
2026 May;11(119):eadz0348. PMID: 42066062 -
Mol Cell
Histone phosphorylation integrates the hepatic glucagon-PKA-CREB gluconeogenesis program in response to fasting. [Abstract]2023 Apr 6;83(7):1093-1108.e8. PMID: 36863348 -
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Theranostics
Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. [Abstract]2021 Mar 24;11(12):5650-5674. PMID: 33897873 -
Theranostics
CXCL12-mediated HOXB5 overexpression facilitates Colorectal Cancer metastasis through transactivating CXCR4 and ITGB3. [Abstract]2021 Jan 1;11(6):2612-2633. PMID: 33456563 -
Acta Pharm Sin B
GRK2 mediated degradation of SAV1 initiates hyperplasia of fibroblast-like synoviocytes in rheumatoid arthritis. [Abstract]2024 Mar;14(3):1222-1240. PMID: 38486990 -
Genes Dis
2020 Nov 21;8(6):867-881. PMID: 34522714 -
J Exp Clin Cancer Res
GPR65 sensing tumor-derived lactate induces HMGB1 release from TAM via the cAMP/PKA/CREB pathway to promote glioma progression. [Abstract]2024 Apr 4;43(1):105. PMID: 38576043 -
J Exp Clin Cancer Res
Efficacy and mechanism of the combination of PARP and CDK4/6 inhibitors in the treatment of triple-negative breast cancer. [Abstract]2021 Apr 8;40(1):122. PMID: 33832512 -
Adv Sci (Weinh)
Osmotic Tension Asymmetry Drives Electrotactic Migration via PDLIM7-Polarized Microfilament Coordination in Breast Cancer Cells. [Abstract]2025 Dec 22:e15246. PMID: 41431193 -
Adv Sci (Weinh)
Calhm6 Governs Macrophage Polarization Through Chp1-Camk4-Creb1 Axis and Ectosomal Delivery in Inflammatory Responses. [Abstract]2025 Sep 26:e02395. PMID: 40999918 -
Adv Sci (Weinh)
Microbiota-Derived Inosine Suppresses Systemic Autoimmunity via Restriction of B Cell Differentiation and Migration. [Abstract]2025 May;12(20):e2409837. PMID: 40289872 -
Adv Sci (Weinh)
Targeting the E Prostanoid Receptor EP4 Mitigates Cardiac Fibrosis Induced by β-Adrenergic Activation. [Abstract]2025 Mar;12(12):e2413324. PMID: 39921269 -
Adv Sci (Weinh)
Granulosa Cell-Layer Stiffening Prevents Escape of Mural Granulosa Cells from the Post-Ovulatory Follicle. [Abstract]2024 Sep;11(33):e2403640. PMID: 38946588 -
Adv Sci (Weinh)
2021 Dec;8(24):e2100808. PMID: 34719888 -
Biomaterials
Self-amplified ROS production from fatty acid oxidation enhanced tumor immunotherapy by atorvastatin/PD-L1 siRNA lipopeptide nanoplexes. [Abstract]2022 Dec:291:121902. PMID: 36371945 -
Neuro Oncol
A disintegrin and metalloproteinase 22 activates integrin β1 through its disintegrin domain to promote the progression of pituitary adenoma. [Abstract]2024 Jan 5;26(1):137-152. PMID: 37555799 -
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Cell Death Dis
Activation of AKT via a dual mechanism enhances the susceptibility of melanoma cells to glucose deprivation. [Abstract]2025 Aug 7;16(1):595. PMID: 40774947 -
Cell Death Dis
S1PR3-driven positive feedback loop sustains STAT3 activation and keratinocyte hyperproliferation in psoriasis. [Abstract]2025 Jan 20;16(1):31. PMID: 39833165 -
Cell Death Dis
2024 Sep 30;15(9):699. PMID: 39349432 -
Int J Biol Sci
Chronic Stress-induced Serotonin Impairs Intestinal Epithelial Cell Mitochondrial Biogenesis via the AMPK-PGC-1α Axis. [Abstract]2024 Aug 19;20(11):4476-4495. PMID: 39247815 -
Int J Biol Sci
Chronic stress promotes colorectal cancer progression by enhancing glycolysis through β2-AR/CREB1 signal pathway. [Abstract]2023 Apr 2;19(7):2006-2019. PMID: 37151872
H-89 dihydrochloride purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2023 Apr 2;19(7):2006-2019. [Abstract]
H-89 dihydrochloride (H-89; 10 μM; 2 h) blocks the epinephrine-induced phosphorylation of CREB1 in MC38 and HT-29 cells.
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Cell Commun Signal
Intestinal epithelial PIEZO1 regulates macrophage-to-myofibroblast transition via epithelial-derived TGF-α signaling in Crohn's disease. [Abstract]2026 May 28. PMID: 42210253 -
Cell Commun Signal
Olanzapine suppresses mPFC activity-norepinephrine releasing to alleviate CLOCK-enhanced cancer stemness under chronic stress. [Abstract]2024 Jul 25;22(1):375. PMID: 39054537 -
Cell Commun Signal
Receptor activity-modifying protein 1 regulates mouse skin fibroblast proliferation via the Gαi3-PKA-CREB-YAP axis. [Abstract]2022 Apr 12;20(1):52. PMID: 35413847 -
Cell Commun Signal
Zinc-mediated activation of CREB pathway in proliferation of pulmonary artery smooth muscle cells in pulmonary hypertension. [Abstract]2021 Oct 11;19(1):103. PMID: 34635097 -
J Neuroinflammation
IL-1β+ tumor-associated macrophages accelerate glioblastoma progression by amplifying the PGE2-EP4 signaling. [Abstract]2025 Oct 14;22(1):233. PMID: 41088217 -
NPJ Biofilms Microbiomes
Gut microbiota-mediated secondary bile acid alleviates Staphylococcus aureus-induced mastitis through the TGR5-cAMP-PKA-NF-κB/NLRP3 pathways in mice. [Abstract]2023 Feb 8;9(1):8. PMID: 36755021 -
Phytomedicine
Z-Ligustilide's antidepressant effects in adolescent depression involve the PTEN-induced putative kinase 1 (PINK1) - protein kinase A (PKA)- Na+/Ca2+ exchanger (NCLX) pathway, facilitating mitochondrial endosymbiosis and promoting mitophagy in microglia. [Abstract]2025 Dec:149:157494. PMID: 41218379 -
Phytomedicine
Shouhui Tongbian Capsule ameliorates obesity by enhancing energy consumption and promoting lipolysis via cAMP-PKA pathway. [Abstract]2025 Mar:138:156375. PMID: 39848021 -
Mater Today Bio
PKA activation rescues myocardial injury elicited by silica nanoparticles through improving oxidative stress, mitochondrial health, and copper homeostasis. [Abstract]2026 Mar 10:37:103021. PMID: 41890951 -
Diabetologia
Harmine selectively enhances human beta cell identity markers and function via protein kinase A pathways. [Abstract]2026 Sep 21. PMID: 42766004 -
Cell Death Discov
The LTB4-BLT1 axis attenuates influenza-induced lung inflammation by suppressing NLRP3 activation. [Abstract]2025 Apr 6;11(1):148. PMID: 40189592 -
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Proc Natl Acad Sci U S A
A PKA-selective inhibitor captures an open but more ordered conformation of the PKA catalytic subunit. [Abstract]2026 May 12;123(19):e2536312123. PMID: 42096309 -
Environ Health (Wash)
Effects on Synaptic Plasticity Markers in Fetal Mice and HT22 Neurons upon F-53B Exposure: The Role of PKA Cytoplasmic Retention. [Abstract]2024 Aug 16;2(11):776-785. PMID: 39568694 -
Int J Biol Macromol
Effect and mechanism of maternal prolactin levels on depressive-like behavior in prenatally stressed offspring. [Abstract]2026 Jan;340(Pt 1):150002. PMID: 41485664 -
Int J Biol Macromol
Genome-wide identification and expression analysis of neuropeptide G protein-coupled receptors in Urechis unicinctus (Annelida, Echiura). [Abstract]2025 May;310(Pt 4):143548. PMID: 40294673 -
Small Methods
An Integrated Microcurrent Delivery System Facilitates Human Parathyroid Hormone Delivery for Enhancing Osteoanabolic Effect. [Abstract]2025 Mar;9(3):e2401144. PMID: 39420694 -
Microbiol Res
The Arf-GAPs, AoAge1 and AoAge2, regulate diverse cellular processes, conidiation, trap formation, and secondary metabolism in Arthrobotrys oligospora. [Abstract]2024 Aug:285:127779. PMID: 38810485 -
Anim Nutr
DHA induces adipocyte lipolysis through endoplasmic reticulum stress and the cAMP/PKA signaling pathway in grass carp (Ctenopharyngodon idella). [Abstract]2022 Dec 31:13:185-196. PMID: 37123617 -
Arch Pharm Res
Tirzepatide mitigates atherosclerosis progression and modulates oxLDL-mediated proatherogenic effects in macrophages: evidence for M1/M2 homeostasis restoration. [Abstract]2026 Apr;49(4):554-574. PMID: 41904760 -
Phytother Res
Protective effects of 4-geranyloxy-2,6-dihydroxybenzophenonel on DSS-induced ulcerative colitis in mice via regulation of cAMP/PKA/CREB and NF-κB signaling pathways. [Abstract]2023 Apr;37(4):1330-1345. PMID: 36428266 -
Chem Sci
2019 Dec 6;11(4):1132-1139. PMID: 34084370 -
Free Radic Biol Med
METTL3 deficiency leads to ovarian insufficiency due to IL-1β overexpression in theca cells. [Abstract]2024 May 31:222:72-84. PMID: 38825211 -
Clin Transl Med
Activation of Toll-like receptor 7 provides cardioprotection in septic cardiomyopathy-induced systolic dysfunction. [Abstract]2021 Jan;11(1):e266. PMID: 33463061 -
Stem Cell Res Ther
Mesenchymal stem cells combined with liraglutide relieve acute lung injury through apoptotic signaling restrained by PKA/β-catenin. [Abstract]2020 May 19;11(1):182. PMID: 32429994 -
Stem Cell Res Ther
Effect of hCMSCs and liraglutide combination in ALI through cAMP/PKAc/β-catenin signaling pathway. [Abstract]2020 Jan 3;11(1):2. PMID: 31900217 -
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Cell Rep
Targeting EP2/EP4-driven expansion of suppressive VSIG4high macrophages overcomes immunotherapy resistance in colorectal cancer. [Abstract]2026 May 28;45(6):117450. PMID: 42213779 -
Cell Rep
2022 Oct 18;41(3):111498. PMID: 36261001 -
Cell Rep
Gut microbiota-mediated secondary bile acids regulate dendritic cells to attenuate autoimmune uveitis through TGR5 signaling. [Abstract]2021 Sep 21;36(12):109726. PMID: 34551302 -
Aging Cell
nNOS-CAPON interaction mediates amyloid-β-induced neurotoxicity, especially in the early stages. [Abstract]2018 Jun;17(3):e12754. PMID: 29577585 -
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Brain Behav Immun
NR2B-CaMKII signaling in the dentate gyrus driven by astrocytic P2Y1Rs mediates the antidepressant effect of low-dose LPS. [Abstract]2026 Jul:135:106529. PMID: 41796645 -
Transl Psychiatry
TGR5 dysfunction underlies chronic social defeat stress via cAMP/PKA signaling pathway in the hippocampus. [Abstract]2025 Oct 6;15(1):366. PMID: 41053031 -
Brain Behav Immun
Dexamethasone attenuates neuropathic pain through spinal microglial expression of dynorphin A via the cAMP/PKA/p38 MAPK/CREB signaling pathway. [Abstract]2024 Jul:119:36-50. PMID: 38555991 -
Brain Behav Immun
Gut microbiota-mediated ursodeoxycholic acids regulate the inflammation of microglia through TGR5 signaling after MCAO. [Abstract]2024 Jan:115:667-679. PMID: 37989444 -
Chin Med
Electroacupuncture promotes BDNF-dependent neurogenesis via microglial reprogramming in a chronic stress model. [Abstract]2026 Feb 3;21(1):62. PMID: 41634791 -
J Med Chem
Discovery of Novel P2Y14R Inhibitors for Ameliorating Liver Fibrosis by Suppressing Hepatic Stellate Cell Activation. [Abstract]2025 Nov 13;68(21):23277-23299. PMID: 41178760 -
Sci Signal
Airway relaxation mechanisms and structural basis of osthole for improving lung function in asthma. [Abstract]2020 Nov 24;13(659):eaax0273. PMID: 33234690 -
Cancer Cell Int
Phenyllactic acid promotes cell migration and invasion in cervical cancer via IKK/NF-κB-mediated MMP-9 activation. [Abstract]2019 Sep 23:19:241. PMID: 31572058 -
J Agric Food Chem
Sargassum fusiforme Fucoidan Ameliorates Obesity-Associated Metabolic Dysfunction via a Tauroursodeoxycholic Acid-Mediated TGR5-cAMP-PKA Signaling Pathway. [Abstract]2025 Aug 13;73(32):20235-20253. PMID: 40758868 -
J Agric Food Chem
Sesquiterpenoids from Alpinia oxyphylla with GLP-1 Stimulative Effects through Ca2+/CaMKII and PKA Pathways and Multiple-Enzyme Inhibition. [Abstract]2023 Nov 1;71(43):16148-16159. PMID: 37871265 -
CNS Neurosci Ther
Inhibition of ADORA3 promotes microglial phagocytosis and alleviates chronic ischemic white matter injury. [Abstract]2024 May;30(5):e14742. PMID: 38715283 -
Ecotoxicol Environ Saf
Role of PKA/CREB/BDNF signaling in PM2.5-induced neurodevelopmental damage to the hippocampal neurons of rats. [Abstract]2021 May:214:112005. PMID: 33640725 -
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Biochem Pharmacol
Calcitonin gene-related peptide/protein kinase A: A novel pathway to regulate patency of ductus arteriosus. [Abstract]2026 Jun:248:117840. PMID: 41724283 -
J Bone Miner Res
Lgr4 promotes aerobic glycolysis and differentiation in osteoblasts via the canonical Wnt/β-catenin pathway. [Abstract]2021 Aug;36(8):1605-1620. PMID: 33950533 -
Life Sci
Mechanistic insights into PPARγ's role in suppressing collagen-driven platelet activation. [Abstract]2026 Jan 15:385:124138. PMID: 41354414 -
Life Sci
Polymyxin B induces pigmentation by upregulating ATG2A-ERK/CREB-MITF-PMEL17 signaling axis. [Abstract]2025 May 15:369:123553. PMID: 40074142 -
Life Sci
Genistein accelerates glucose catabolism via activation the GPER-mediated cAMP/PKA-AMPK signaling pathway in broiler chickens. [Abstract]2022 Aug 15:303:120676. PMID: 35640778 -
Cells
Glucagon Promotes Gluconeogenesis through the GCGR/PKA/CREB/PGC-1α Pathway in Hepatocytes of the Japanese Flounder Paralichthys olivaceus. [Abstract]2023 Apr 6;12(7):1098. PMID: 37048171 -
Nutrients
2020 Jan 26;12(2):329. PMID: 31991934 -
Eur J Pharmacol
Exendin-4, a glucagon-like peptide-1 receptor agonist, regulates ductus arteriosus by vasodilation and anti-remodeling through the PKA pathway. [Abstract]2024 Dec 15:985:177106. PMID: 39515563 -
Nutr Diabetes
Intrauterine hyperglycaemia during late gestation caused mitochondrial dysfunction in skeletal muscle of male offspring through CREB/PGC1A signaling. [Abstract]2024 Jul 23;14(1):56. PMID: 39043630 -
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Int Immunopharmacol
Phenylacetaldehyde attenuates Cutibacterium acnes-induced inflammation in keratinocytes and monocytes. [Abstract]2025 Jun 17:158:114885. PMID: 40383096 -
Int Immunopharmacol
Hypersampsonone H attenuates ulcerative colitis via inhibition of PDE4 and regulation of cAMP/PKA/CREB signaling pathway. [Abstract]2024 Feb 15:128:111490. PMID: 38218008 -
Int J Mol Sci
Alkaline Dilution Alters Sperm Motility in Dairy Goat by Affecting sAC/cAMP/PKA Pathway Activity. [Abstract]2023 Jan 16;24(2):1771. PMID: 36675287 -
Int J Mol Sci
Salicylate Sodium Suppresses Monocyte Chemoattractant Protein-1 Production by Directly Inhibiting Phosphodiesterase 3B in TNF-α-Stimulated Adipocytes. [Abstract]2022 Dec 24;24(1):320. PMID: 36613764 -
Int J Mol Sci
Asprosin in the Paraventricular Nucleus Induces Sympathetic Activation and Pressor Responses via cAMP-Dependent ROS Production. [Abstract]2022 Oct 20;23(20):12595. PMID: 36293450 -
Int J Mol Sci
AWRK6, a Novel GLP-1 Receptor Agonist, Attenuates Diabetes by Stimulating Insulin Secretion. [Abstract]2018 Oct 7;19(10):3053. PMID: 30301245 -
Glia
RetSat Knockout Mitigates Hypoxia-Induced Microglial Activation by Enhancing Lipid Droplets Degradation. [Abstract]2026 Feb;74(2):e70118. PMID: 41388745 -
Invest Ophthalmol Vis Sci
Epac1 Inhibits Orbital Fibroblast Activation to Ameliorate Thyroid-Associated Orbitopathy-Like Features Through the JAK/STAT Signaling Pathway. [Abstract]2025 Jul 1;66(9):68. PMID: 40736178 -
Mol Neurobiol
Gastrodin Ameliorates Post-Stroke Depressive-Like Behaviors Through Cannabinoid-1 Receptor-Dependent PKA/RhoA Signaling Pathway. [Abstract]2025 Jan;62(1):366-385. PMID: 38856794 -
Am J Physiol Cell Physiol
2024 Jun 1;326(6):C1683-C1696. PMID: 38646785 -
BMC Biol
GnRH-driven FTO-mediated RNA m6A modification promotes gonadotropin synthesis and secretion. [Abstract]2024 May 3;22(1):104. PMID: 38702712 -
Front Pharmacol
Simvastatin Enhances Activity and Trafficking of α7 Nicotinic Acetylcholine Receptor in Hippocampal Neurons Through PKC and CaMKII Signaling Pathways. [Abstract]2018 Apr 12:9:362. PMID: 29706890 -
Chem Biol Interact
Oxyphylla A exerts antiparkinsonian effects by ameliorating 6-OHDA-induced mitochondrial dysfunction and dyskinesia in vitro and in vivo. [Abstract]2024 Nov 1:403:111224. PMID: 39233265 -
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J Lipid Res
2026 Feb;67(2):100981. PMID: 41565113 -
Sci Rep
2025 Oct 16;15(1):36270. PMID: 41102391 -
Neuropharmacology
GPR139-mediated cholinergic circuit from the medial septum to the ventral hippocampal CA3 modulates cognitive impairment in Alzheimer's disease. [Abstract]2026 Nov 1:298:111043. PMID: 42218995 -
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Neuropharmacology
GLP-1 modulated the firing activity of nigral dopaminergic neurons in both normal and parkinsonian mice. [Abstract]2024 Jul 1:252:109946. PMID: 38599494 -
J Cell Mol Med
Effect of Curcuma longa extract on reproduction function in mice and testosterone production in Leydig cells. [Abstract]2024 Apr;28(8):e18303. PMID: 38613362 -
J Cell Mol Med
Maresin Conjugates in Tissue Regeneration 1 improves alveolar fluid clearance by up-regulating alveolar ENaC, Na, K-ATPase in lipopolysaccharide-induced acute lung injury. [Abstract]2020 Apr;24(8):4736-4747. PMID: 32160403 -
Prog Neurobiol
2021 Jul:202:102041. PMID: 33766679 -
Poult Sci
PKA and PKC signaling pathways mediate vitamin D₃-regulated intestinal phosphorus absorption in broiler chickens. [Abstract]2026 Mar 12;105(6):106762. PMID: 41855802 -
iScience
The matricellular protein Fibulin-5 regulates β-cell proliferation in an autocrine/paracrine manner. [Abstract]2025 Jan 21;28(2):111856. PMID: 39995864 -
Acta Biochim Biophys Sin (Shanghai)
Evolution of GCGR family ligand-receptor extensive cross-interaction systems suggests a therapeutic direction for hyperglycemia in mammals. [Abstract]2023 Nov 15;55(12):1855-1863. PMID: 37969012 -
iScience
Pleiotropic roles of Ras GTPases in the nematode-trapping fungus Arthrobotrys oligospora identified through multi-omics analyses. [Abstract]2021 Jul 8;24(8):102820. PMID: 34337364 -
J Periodontol
Intermittent parathyroid hormone promotes cementogenesis in a PKA- and ERK1/2-dependent manner. [Abstract]2019 Sep;90(9):1002-1013. PMID: 31026057 -
Front Physiol
Endoplasmic Reticulum Stress/Ca2+-Calmodulin-Dependent Protein Kinase/Signal Transducer and Activator of Transcription 3 Pathway Plays a Role in the Regulation of Cellular Zinc Deficiency in Myocardial Ischemia/Reperfusion Injury. [Abstract]2022 Jan 5:12:736920. PMID: 35069232 -
Front Physiol
Roles of Endothelial Motilin Receptor and Its Signal Transduction Pathway in Motilin-Induced Left Gastric Artery Relaxation in Dogs. [Abstract]2021 Oct 28;12:770430. PMID: 34777026 -
Biol Trace Elem Res
2021 Jul;199(7):2687-2694. PMID: 32926327 -
FASEB J
Morinda officinalis oligosaccharides ameliorate depressive-like behaviors in poststroke rats through upregulating GLUT3 to improve synaptic activity. [Abstract]2020 Oct;34(10):13376-13395. PMID: 32812265 -
J Neurosci
Olig2-Targeted G-Protein-Coupled Receptor Gpr17 Regulates Oligodendrocyte Survival in Response to Lysolecithin-Induced Demyelination. [Abstract]2016 Oct 12;36(41):10560-10573. PMID: 27733608
H-89 dihydrochloride purchased from MedChemExpress. Usage Cited in: J Neurosci. 2016 Oct 12;36(41):10560-10573. [Abstract]
The inhibition of PKA signaling with PKA inhibitor H89 reduces cell viability observed after Pranlukast treatment.
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Korean J Pain
Perampanel ameliorates nitroglycerin-induced migraine through inhibition of the cAMP/PKA/CREB signaling pathway in the trigeminal ganglion in rats. [Abstract]2023 Jul 1;36(3):335-346. PMID: 37394274 -
Immunotargets Ther
Myeloid Cell-Derived Thrombospondin-1 Mediates Anti-Metastatic Effects of Histamine Dihydrochloride in B16 Melanoma. [Abstract]2026 Jun 6:15:596703. PMID: 42281617 -
Proteomics
Dynamic Phosphoproteomic Profiling Captured Early Signaling Events for Cold Acclimation in Larval Zebrafish. [Abstract]2026 May;26(5):69-85. PMID: 41906457 -
J Biol Chem
HIV-1 exposure promotes PKG1-mediated phosphorylation and degradation of stathmin to increase epithelial barrier permeability. [Abstract]2021 Jan-Jun:296:100644. PMID: 33839152 -
Development
The neuropeptide FILa modulates larval settlement and ciliary function in the echiuran worm Urechis unicinctus. [Abstract]2026 Apr 15;153(8):dev205191. PMID: 41958431 -
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BMC Genomics
Identification of the principal neuropeptide MIP and its action pathway in larval settlement of the echiuran worm Urechis unicinctus. [Abstract]2024 Apr 3;25(1):337. PMID: 38641568 -
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Bone
hPTH(3-34)(29-34) selectively activated PKC and mimicked osteoanabolic effects of hPTH(1-34). [Abstract]2020 Jun:135:115326. PMID: 32200023 -
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J Orthop Surg Res
ITGB1 alleviates osteoarthritis by inhibiting cartilage inflammation and apoptosis via activating cAMP pathway. [Abstract]2023 Nov 8;18(1):849. PMID: 37941009 -
Endocrinology
2023 Mar 13;164(5):bqad042. PMID: 36929849 -
Mol Brain
Meningitic Escherichia coli α-hemolysin aggravates blood-brain barrier disruption via targeting TGFβ1-triggered hedgehog signaling. [Abstract]2021 Jul 19;14(1):116. PMID: 34281571 -
Int J Biochem Cell Biol
Downregulation of Pannexin-1 attenuates sepsis-induced acute lung injury via activation of the cAMP/PKA pathway. [Abstract]2026 Jun-Jul:195-196:106941. PMID: 41850417 -
Peptides
2026 Jan:195:171463. PMID: 41485715 -
J Gastroenterol Hepatol
Targeting DRP1Ser637 Phosphorylation Alleviates Acidic Bile Salt-Induced NF-κB Activation via Mitochondrial Protection. [Abstract]2025 Jul 31. PMID: 40741897 -
BMC Pharmacol Toxicol
Bisdemethoxycurcumin alleviates LPS-induced acute lung injury via activating AMPKα pathway. [Abstract]2023 Nov 20;24(1):63. PMID: 37986186 -
Peptides
Neuropeptide Y promotes hepatic apolipoprotein A1 synthesis and secretion through neuropeptide Y Y5 receptor. [Abstract]2022 Aug:154:170824. PMID: 35660638 -
Exp Cell Res
Down-regulation of A3AR signaling by IL-6-induced GRK2 activation contributes to Th17 cell differentiation. [Abstract]2021 Feb 15;399(2):112482. PMID: 33434531 -
Arch Biochem Biophys
Identification and characterization of human PEIG-1/GPRC5A as a 12-O-tetradecanoyl phorbol-13-acetate (TPA) and PKC-induced gene. [Abstract]2020 Jul 15;687:108375. PMID: 32339486 -
Andrology
Ubiquitin-mediated immunoglobulin-binding protein 1 degradation promotes autophagy and sperm capacitation in vitro. [Abstract]2025 Jul 2. PMID: 40600667 -
Front Oncol
SUZ12 Loss Amplifies the Ras/ERK Pathway by Activating Adenylate Cyclase 1 in NF1-Associated Neurofibromas. [Abstract]2021 Oct 6;11:738300. PMID: 34692515 -
Psychoneuroendocrinology
2020 Jul:117:104680. PMID: 32387876 -
Infect Immun
Phosphothreonine Lyase Promotes p65 Degradation in a Mitogen-Activated Protein Kinase/Mitogen- and Stress-Activated Protein Kinase 1-Dependent Manner. [Abstract]2018 Dec 19;87(1):e00508-18. PMID: 30396897
H-89 dihydrochloride purchased from MedChemExpress. Usage Cited in: Infect Immun. 2018 Dec 19;87(1):e00508-18. [Abstract]
J774A.1 cells are pre-treated with 473 U0126 (10 μM), SB208530 (10 μM), SP600125 (10 μM), or H89 (50 μM), then infected with ΔeseH E. piscicida at the MOI of 10 for 3.5 h. Cell lysates were probed with anti-phospho-p65 (Ser276), anti-p65, and anti-β-actin antibodies.
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Biol Reprod
Activation of cAMP/PKA and ERK signaling pathways and potential role of Phoenixin in the reproductive axis of spotted scat (Scatophagus argus)†. [Abstract]2025 Sep 18:ioaf213. PMID: 40973150 -
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Toxicol Lett
Adenosine receptor A2B mediates alcoholic hepatitis by regulating cAMP levels and the NF-KB pathway. [Abstract]2022 Apr 15:359:84-95. PMID: 35143882 -
Neurogastroenterol Motil
Acute stress induces visceral hypersensitivity via glucocorticoid receptor-mediated membrane insertion of TRPM8: Involvement of a non-receptor tyrosine kinase Pyk2. [Abstract]2020 Oct;32(10):1514-1528. PMID: 32391653 -
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Fitoterapia
Oligostilbenes from the seeds of Paeonia lactiflora as potent GLP-1 secretagogues targeting TGR5 receptor. [Abstract]2022 Nov:163:105336. PMID: 36257378 -
PeerJ
Icariside II induces rapid phosphorylation of endothelial nitric oxide synthase via multiple signaling pathways. [Abstract]2022 Oct 25:10:e14192. PMID: 36312762 -
J Bone Miner Metab
2021 Sep;39(5):748-756. PMID: 33829324 -
Reprod Sci
Role of the Glycogen Synthase Kinase 3-Cyclic AMP/Protein Kinase A in the Immobilization of Human Sperm by Tideglusib. [Abstract]2023 Apr;30(4):1281-1290. PMID: 36207578 -
Vet Microbiol
Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) induces IL-6 production through TAK-1/JNK/AP-1 and TAK-1/NF-κB signaling pathways. [Abstract]2021 May:256:109061. PMID: 33836390 -
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Mol Reprod Dev
CD55 is upregulated by cAMP/PKA/AKT and modulates human decidualization via Src and ERK pathway and decidualization-related genes. [Abstract]2022 May;89(5-6):256-268. PMID: 35474595 -
Comp Biochem Physiol B Biochem Mol Biol
Molecular and functional characterization of the kisspeptin receptor Kissr3 in the large yellow croaker (Larimichthys crocea). [Abstract]2026 Jun-Jul:284:111225. PMID: 41912081 -
Eur J Neurosci
2025 Jul;62(2):e70198. PMID: 40673719 -
Biochem Biophys Res Commun
Olodaterol promotes thermogenesis in brown adipocytes via regulation of the β2-AR/cAMP/PKA signaling pathway. [Abstract]2024 Apr 9:703:149689. PMID: 38382361 -
Purinergic Signal
Lysophosphatidylcholine induces adenosine release from macrophages via TRPM7-mediated mitochondrial activation. [Abstract]2022 Sep;18(3):317-343. PMID: 35779163 -
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J Neurophysiol
cAMP-dependent protein kinase signaling is required for ( 2R,6R)-hydroxynorketamine to potentiate hippocampal glutamatergic transmission. [Abstract]2024 Jan 1;131(1):64-74. PMID: 38050689 -
Exp Ther Med
Melatonin alleviates traumatic brain injury-induced anxiety-like behaviors in rats: Roles of the protein kinase A/cAMP-response element binding signaling pathway. [Abstract]2022 Apr;23(4):248. PMID: 35261620 -
Gen Comp Endocrinol
Characterization of serotonin receptors Aj5-HTR2 and Aj5-HTR7 reveals potential roles in circadian regulation of Apostichopus japonicus. [Abstract]2026 Mar:379:114913. PMID: 41819218 -
Eur J Histochem
Overexpression of GPER1 suppressed esophageal carcinoma growth via activating cAMP pathway. [Abstract]2026 Jan 26;70(1). PMID: 41586754 -
Biol Pharm Bull
Adenosine Stimulates Beating of Neonatal Brain-Derived Cilia through Adenosine A2B Receptor on the Cilia and Activation of Protein Kinase A Pathway. [Abstract]2024;47(6):1113-1118. PMID: 38839362 -
J Int Med Res
Progesterone activates the cyclic AMP-protein kinase A signalling pathway by upregulating ABHD2 in fertile men. [Abstract]2021 Mar;49(3):300060521999527. PMID: 33752482 -
J Spinal Cord Med
Electroacupuncture regulates detrusor connexin 43 via protein kinase A pathway in a rat model of neurogenic bladder after spinal cord injury. [Abstract]2026 Jun 4:1-15. PMID: 42240616 -
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bioRxiv
Enhanced kinase translocation reporters for simultaneous real-time measurement of PKA, ERK, and Ca2. [Abstract]2024 Oct 2:2024.09.30.615856. PMID: 39411162 -
bioRxiv
Rapid Hippocampal Synaptic Potentiation Induced by Ketamine Metabolite ( 2R , 6R )-Hydroxynorketamine Persistently Primes Synaptic Plasticity. [Abstract]2024 Oct 22:2024.10.18.619152. PMID: 39484512 -
Heliyon
CD38 symmetric dimethyl site R58 promotes malignant tumor cell immune escape by regulating the cAMP-GSK3β-PD-L1 axis. [Abstract]2024 Sep 19;10(19):e37958. PMID: 39386836 -
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Proteome Sci
Proteomic analysis of neonatal mouse hearts shows PKA functions as a cardiomyocyte replication regulator. [Abstract]2023 Oct 11;21(1):16. PMID: 37821903 -
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Elife
2023 Jan 16:12:e81438. PMID: 36645741 -
Sci Total Environ
Silica nanoparticles perturbed mitochondrial dynamics and induced myocardial apoptosis via PKA-DRP1-mitochondrial fission signaling. [Abstract]2022 Oct 10:842:156854. PMID: 35750168 -
Biomed Pharmacother
Teriparatide induces angiogenesis in ischemic cerebral infarction zones of rats through AC/PKA signaling and reduces ischemia-reperfusion injury. [Abstract]2022 Apr:148:112728. PMID: 35220030 -
Evid Based Complement Alternat Med
The Inhibition Effects of Shenmai Injection on Acetylcholine-Induced Catecholamine Synthesis and Secretion by Modulating Nicotinic Acetylcholine Receptor Ion Channels in Cultured Bovine Adrenal Medullary Cells. [Abstract]2020 Dec 15:2020:8514926. PMID: 33456492 -
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Aging (Albany NY)
Fibroblast growth factor 2 contributes to the effect of salidroside on dendritic and synaptic plasticity after cerebral ischemia/reperfusion injury. [Abstract]2020 Jun 9;12(11):10951-10968. PMID: 32518214 -
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Aging (Albany NY)
Heme oxygenase-1 ameliorates oxidative stress-induced endothelial senescence via regulating endothelial nitric oxide synthase activation and coupling. [Abstract]2018 Jul 24;10(7):1722-1744. PMID: 30048241
H-89 dihydrochloride purchased from MedChemExpress. Usage Cited in: Aging (Albany NY). 2018 Jul 24;10(7):1722-1744. [Abstract]
Expressions of p-eNOS (Ser1177) and total eNOS are measured in HUVECs treated with or without H2O2 and Hemin in the presence or absence of Akt inhibitor MK-2206, PKA inhibitor H-89 or AMPK inhibitor Compound C (CP-C).
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (192.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 5 mg/mL (9.63 mM; ultrasonic and warming and heat to 80°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% Saline
Solubility: 5 mg/mL (9.63 mM); Suspended solution; Need ultrasonic
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 1% DMSO 99% Saline
Solubility: ≥ 0.55 mg/mL (1.06 mM); Clear solution
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (280 KB)
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SDS (394 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Chijiwa T, et al. Inhibition of forskolin-induced neurite outgrowth and protein phosphorylation by a newly synthesized selective inhibitor of cyclic AMP-dependent protein kinase, N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H-89), of PC12D pheochromocytoma cells. J Biol Chem. 1990 Mar 25;265(9):5267-72. [Content Brief]
[2]. Blazev R, et al. Effects of the PKA inhibitor H-89 on excitation-contraction coupling in skinned and intact skeletal muscle fibres. J Muscle Res Cell Motil. 2001;22(3):277-86. [Content Brief]
[3]. Hosseini-Zare MS, et al. Effects of BL-191 and H-89 on epileptogenic activity of bucladesine in pentylenetetrazol-treated mice. Eur J Pharmacol. 2011 Nov 30;670(2-3):464-70. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.9257 mL | 9.6287 mL | 19.2574 mL | 48.1436 mL |
| 5 mM | 0.3851 mL | 1.9257 mL | 3.8515 mL | 9.6287 mL | |
| DMSO | 10 mM | 0.1926 mL | 0.9629 mL | 1.9257 mL | 4.8144 mL |
| 15 mM | 0.1284 mL | 0.6419 mL | 1.2838 mL | 3.2096 mL | |
| 20 mM | 0.0963 mL | 0.4814 mL | 0.9629 mL | 2.4072 mL | |
| 25 mM | 0.0770 mL | 0.3851 mL | 0.7703 mL | 1.9257 mL | |
| 30 mM | 0.0642 mL | 0.3210 mL | 0.6419 mL | 1.6048 mL | |
| 40 mM | 0.0481 mL | 0.2407 mL | 0.4814 mL | 1.2036 mL | |
| 50 mM | 0.0385 mL | 0.1926 mL | 0.3851 mL | 0.9629 mL | |
| 60 mM | 0.0321 mL | 0.1605 mL | 0.3210 mL | 0.8024 mL | |
| 80 mM | 0.0241 mL | 0.1204 mL | 0.2407 mL | 0.6018 mL | |
| 100 mM | 0.0193 mL | 0.0963 mL | 0.1926 mL | 0.4814 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
H-89 dihydrochloride Related Classifications
HY-15979A Related Classifications
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