H-89
Based on 182 publication(s) in Google Scholar
H-89 is a potent and selective inhibitor of cyclic AMP-dependent protein kinase (protein kinase A) with IC50 of 48 nM and has weak inhibition on PKG, PKC, Casein Kinase, and others kinases.
For research use only. We do not sell to patients.
- Purity: 99.96%
- CAS No.: 127243-85-0
- Formula: C20H20BrN3O2S
- Molecular Weight:446.36
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Publications Citing Use of MedChemExpress (MCE) H-89
More- Signal Transduct Target Ther. 2023 Aug 9;8(1):290. [Abstract]
- Cell Metab. 2025 Jan 7;37(1):87-103.e10. [Abstract]
- Cell Metab. 2021 Oct 5;33(10):2021-2039.e8. [Abstract]
- Cell Metab. 2021 Mar 2;33(3):565-580.e7. [Abstract]
- Nat Immunol. 2024 Nov;25(11):2057-2067. [Abstract]
- Nat Metab. 2026 Mar;8(3):704-721. [Abstract]
- Nat Metab. 2024 Apr;6(4):708-723. [Abstract]
- Ann Rheum Dis. 2025 Oct 28:S0003-4967(25)04428-0. [Abstract]
- Cell Mol Immunol. 2023 Feb;20(2):175-188. [Abstract]
- Mol Cell. 2023 Apr 6;83(7):1093-1108.e8. [Abstract]
- ACS Nano. 2025 Jul 1;19(25):22755-22766. [Abstract]
- Nat Commun. 2025 Jul 1;16(1):5425. [Abstract]
- Nat Commun. 2025 Jul 28;16(1):6942. [Abstract]
- Nat Commun. 2023 May 31;14(1):3159. [Abstract]
- Nat Commun. 2022 Nov 4;13(1):6648. [Abstract]
- Acta Pharm Sin B. 2024 Mar;14(3):1222-1240. [Abstract]
- Autophagy. 2026 Mar 15:1-22. [Abstract]
- J Mater Sci Technol. 2023 Jun 30.
- Adv Sci (Weinh). 2025 Dec 22:e15246. [Abstract]
- Adv Sci (Weinh). 2025 Sep 26:e02395. [Abstract]
- Adv Sci (Weinh). 2025 Apr 28:e2409837. [Abstract]
- Adv Sci (Weinh). 2025 Feb 7:e2413324. [Abstract]
- Adv Sci (Weinh). 2024 Jul 1:e2403640. [Abstract]
- Adv Sci (Weinh). 2021 Dec;8(24):e2100808. [Abstract]
- Neuro Oncol. 2024 Jan 5;26(1):137-152. [Abstract]
- Theranostics. 2021 Mar 24;11(12):5650-5674. [Abstract]
- Theranostics. 2021 Jan 1;11(6):2612-2633. [Abstract]
- J Adv Res. 2025 Apr 22:S2090-1232(25)00278-4. [Abstract]
- Biomaterials. 2022 Dec:291:121902. [Abstract]
- J Exp Clin Cancer Res. 2024 Apr 4;43(1):105. [Abstract]
- J Exp Clin Cancer Res. 2021 Apr 8;40(1):122. [Abstract]
- Mater Today Bio. 2026 Mar 10:37:103021. [Abstract]
- J Neuroinflammation. 2025 Oct 14;22(1):233. [Abstract]
- Int J Biol Sci. 2024 Aug 19;20(11):4476-4495. [Abstract]
- Int J Biol Sci. 2023 Apr 2;19(7):2006-2019. [Abstract]
- Cell Death Dis. 2025 Aug 7;16(1):595. [Abstract]
- Cell Death Dis. 2025 Jan 20;16(1):31. [Abstract]
- Cell Death Dis. 2024 Sep 30;15(9):699. [Abstract]
- Genes Dis. 2020 Nov 21;8(6):867-881. [Abstract]
- NPJ Biofilms Microbiomes. 2023 Feb 8;9(1):8. [Abstract]
- Proc Natl Acad Sci U S A. 2026 May 12;123(19):e2536312123. [Abstract]
- Proc Natl Acad Sci U S A. 2026 Jul 7;123(27):e2523789123.
- Small Methods. 2025 Mar;9(3):e2401144. [Abstract]
- Cell Commun Signal. 2024 Jul 25;22(1):375. [Abstract]
- Cell Commun Signal. 2022 Apr 12;20(1):52. [Abstract]
- Cell Commun Signal. 2021 Oct 11;19(1):103. [Abstract]
- Int J Biol Macromol. 2026 Jan;340(Pt 1):150002. [Abstract]
- Int J Biol Macromol. 2025 Apr 26:143548. [Abstract]
- Phytomedicine. 2025 Dec:149:157494. [Abstract]
- Phytomedicine. 2025 Mar:138:156375. [Abstract]
- Free Radic Biol Med. 2024 May 31:222:72-84. [Abstract]
- Sci Total Environ. 2022 Oct 10:842:156854. [Abstract]
- Brain Behav Immun. 2026 Mar 6:135:106529. [Abstract]
- Brain Behav Immun. 2024 Jul:119:36-50. [Abstract]
- Brain Behav Immun. 2024 Jan:115:667-679. [Abstract]
- Anim Nutr. 2022 Dec 31:13:185-196. [Abstract]
- Biomed Pharmacother. 2022 Apr:148:112728. [Abstract]
- Chem Sci. 2019 Dec 6;11(4):1132-1139. [Abstract]
- Stem Cell Res Ther. 2020 May 19;11(1):182. [Abstract]
- Stem Cell Res Ther. 2020 Jan 3;11(1):2. [Abstract]
- Aging Cell. 2018 Jun;17(3):e12754. [Abstract]
- Cell Death Discov. 2025 Apr 6;11(1):148. [Abstract]
- Microbiol Res. 2024 May 24:285:127779. [Abstract]
- Cell Rep. 2022 Oct 18;41(3):111498. [Abstract]
- Cell Rep. 2021 Sep 21;36(12):109726. [Abstract]
- J Med Chem. 2025 Nov 13;68(21):23277-23299. [Abstract]
- Clin Transl Med. 2021 Jan;11(1):e266. [Abstract]
- Sci Signal. 2020 Nov 24;13(659):eaax0273. [Abstract]
- Elife. 2023 Jan 16:12:e81438. [Abstract]
- Environ Health (Wash). 2024 Aug 16;2(11):776-785. [Abstract]
- Phytother Res. 2023 Apr;37(4):1330-1345. [Abstract]
- Transl Psychiatry. 2025 Oct 6;15(1):366. [Abstract]
- J Agric Food Chem. 2025 Aug 13;73(32):20235-20253. [Abstract]
- J Agric Food Chem. 2023 Nov 1;71(43):16148-16159. [Abstract]
- Prog Neurobiol. 2021 Jul:202:102041. [Abstract]
- Ecotoxicol Environ Saf. 2021 May:214:112005. [Abstract]
- Cancer Cell Int. 2019 Sep 23;19:241. [Abstract]
- J Bone Miner Res. 2021 Aug;36(8):1605-1620. [Abstract]
- Chin Med. 2026 Feb 3;21(1):62. [Abstract]
- Biochem Pharmacol. 2026 Jun:248:117840. [Abstract]
- Chem Biol Interact. 2024 Sep 2:111224. [Abstract]
- Nutr Diabetes. 2024 Jul 23;14(1):56. [Abstract]
- Cells. 2023 Apr 6;12(7):1098. [Abstract]
- Life Sci. 2026 Jan 15:385:124138. [Abstract]
- Glia. 2026 Feb;74(2):e70118. [Abstract]
- Life Sci. 2025 May 15:369:123553. [Abstract]
- Life Sci. 2022 Aug 15:303:120676. [Abstract]
- CNS Neurosci Ther. 2024 May;30(5):e14742. [Abstract]
- Nutrients. 2020 Jan 26;12(2):329. [Abstract]
- Int J Mol Sci. 2023 Jan 16;24(2):1771. [Abstract]
- Int J Mol Sci. 2022 Dec 24;24(1):320. [Abstract]
- Int J Mol Sci. 2022 Oct 20;23(20):12595. [Abstract]
- Int J Mol Sci. 2018 Oct 7;19(10). pii: E3053. [Abstract]
- Front Pharmacol. 2018 Apr 12:9:362. [Abstract]
- Invest Ophthalmol Vis Sci. 2025 Jul 1;66(9):68. [Abstract]
- Int Immunopharmacol. 2025 May 17:158:114885. [Abstract]
- Eur J Pharmacol. 2024 Nov 6:985:177106. [Abstract]
- Am J Physiol Cell Physiol. 2024 Jun 1;326(6):C1683-C1696. [Abstract]
- Int Immunopharmacol. 2024 Feb 15:128:111490. [Abstract]
- BMC Biol. 2024 May 3;22(1):104. [Abstract]
- Immunotargets Ther. 2026 Jun 6:15:596703. [Abstract]
- J Integr Agric. 2023 Feb 24.
- Mol Neurobiol. 2025 Jan;62(1):366-385. [Abstract]
- Poult Sci. 2026 Mar 12;105(6):106762. [Abstract]
- J Cell Mol Med. 2024 Apr;28(8):e18303. [Abstract]
- FASEB J. 2020 Oct;34(10):13376-13395. [Abstract]
- J Cell Mol Med. 2020 Apr;24(8):4736-4747. [Abstract]
- J Lipid Res. 2026 Feb;67(2):100981. [Abstract]
- iScience. 2025 Jan 21;28(2):111856. [Abstract]
- iScience. 2021 Jul 8;24(8):102820. [Abstract]
- J Funct Foods. 2025 Aug.
- J Funct Foods. 2023 Dec, 111, 105902.
- J Neurosci. 2016 Oct 12;36(41):10560-10573. [Abstract]
- Sci Rep. 2025 Oct 16;15(1):36270. [Abstract]
- J Biol Chem. 2021 Jan-Jun:296:100644. [Abstract]
- Aging (Albany NY). 2020 Jun 9;12(11):10951-10968. [Abstract]
- Aging (Albany NY). 2018 Jul 24;10(7):1722-1744. [Abstract]
- J Periodontol. 2019 Sep;90(9):1002-1013. [Abstract]
- Aquac Rep. 2024 Jun, 36, 102110.
- BMC Genomics. 2024 Apr 3;25(1):337. [Abstract]
- Heliyon. 2024 Sep 19;10(19):e37958. [Abstract]
- Biol Trace Elem Res. 2021 Jul;199(7):2687-2694. [Abstract]
- Psychoneuroendocrinology. 2020 Jul;117:104680. [Abstract]
- Bone. 2020 Jun;135:115326. [Abstract]
- Exp Cell Res. 2021 Feb 15;399(2):112482. [Abstract]
- J Gastroenterol Hepatol. 2025 Jul 31. [Abstract]
- Andrology. 2025 Jul 2. [Abstract]
- Acta Biochim Biophys Sin (Shanghai). 2023 Nov 15;55(12):1855-1863. [Abstract]
- Front Physiol. 2022 Jan 5:12:736920. [Abstract]
- Front Physiol. 2021 Oct 28;12:770430. [Abstract]
- Endocrinology. 2023 Mar 13;164(5):bqad042. [Abstract]
- Front Oncol. 2021 Oct 6;11:738300. [Abstract]
- Korean J Pain. 2023 Jul 1;36(3):335-346. [Abstract]
- Biol Reprod. 2025 Sep 18:ioaf213. [Abstract]
- Mol Reprod Dev. 2022 May;89(5-6):256-268. [Abstract]
- Arch Biochem Biophys. 2020 Jul 15;687:108375. [Abstract]
- Peptides. 2026 Jan:195:171463. [Abstract]
- Peptides. 2022 Aug:154:170824. [Abstract]
- Toxicol Lett. 2022 Apr 15:359:84-95. [Abstract]
- Mol Brain. 2021 Jul 19;14(1):116. [Abstract]
- Neurogastroenterol Motil. 2020 Oct;32(10):1514-1528. [Abstract]
- Int J Biochem Cell Biol. 2026 Jun-Jul:195-196:106941. [Abstract]
- J Orthop Surg Res. 2023 Nov 8;18(1):849. [Abstract]
- Infect Immun. 2018 Dec 19;87(1). pii: e00508-1 [Abstract]
- BMC Pharmacol Toxicol. 2023 Nov 20;24(1):63. [Abstract]
- Vet Microbiol. 2021 May:256:109061. [Abstract]
- Fitoterapia. 2022 Nov:163:105336. [Abstract]
- Reprod Sci. 2023 Apr;30(4):1281-1290. [Abstract]
- Eur J Neurosci. 2025 Jul;62(2):e70198. [Abstract]
- PeerJ. 2022 Oct 25:10:e14192. [Abstract]
- Purinergic Signal. 2022 Sep;18(3):317-343. [Abstract]
- Exp Ther Med. 2022 Apr;23(4):248. [Abstract]
- Biochem Biophys Res Commun. 2024 Apr 9:703:149689. [Abstract]
- J Bone Miner Metab. 2021 Sep;39(5):748-756. [Abstract]
- Eur J Histochem. 2026 Jan 26;70(1). [Abstract]
- J Neurophysiol. 2024 Jan 1;131(1):64-74. [Abstract]
- Aquac Res. 2020;00:1-10.
- Comp Biochem Physiol B Biochem Mol Biol. 2026 Jun-Jul:284:111225. [Abstract]
- Gen Comp Endocrinol. 2026 Mar:379:114913. [Abstract]
- Biol Pharm Bull. 2024;47(6):1113-1118. [Abstract]
- Proteome Sci. 2023 Oct 11;21(1):16. [Abstract]
- J Int Med Res. 2021 Mar;49(3):300060521999527. [Abstract]
- Heart Surg Forum. 2024 Mar 17.
- bioRxiv. 2026 Feb 10.
- Res Sq. 2026 Jan 13.
- bioRxiv. 2025 Nov 28.
- AUTHOREA. 2025 May 27.
- bioRxiv. 2025 May 12.
- J Appl Biol Chem. 2025;68:26, 204‒211.
- Res Sq. 2025 Apr 08.
- SSRN. 2025 Apr 10.
- SSRN. 2025 Mar 24.
- Research Square Preprint. 2024 Dec 04.
- bioRxiv. 2024 Oct 22:2024.10.18.619152. [Abstract]
- bioRxiv. 2024 Oct 2:2024.09.30.615856. [Abstract]
- Res Sq. 2024 Jul 15.
- Res Sq. 2024 May 23.
- bioRxiv. 2024 Feb 8.
- Research Square Preprint. 2023 Sep 13.
- Evid Based Complement Alternat Med. 2020 Dec 15:2020:8514926. [Abstract]
- Research Square Preprint. 2020 Oct.
- IJMPNP. 2019,5(1): 8-15.
Customer Validation & Images
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RT-PCR
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WB
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WB
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WB
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Cell Proliferation/Viability Assay
Biological Activity
IC50: 48 nM (protein kinase A)
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
15.7 μM
Compound: H-89
|
Antiproliferative activity against human HCT116 cells after 72 hrs by MTT assay
Antiproliferative activity against human HCT116 cells after 72 hrs by MTT assay
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[PMID: 24389511] |
| HCT-116 | IC50 |
15.7 μM
Compound: H-89
|
Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay
Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay
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[PMID: 24508830] |
| HL-60 | IC50 |
11.4 μM
Compound: H-89
|
Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay
Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay
|
[PMID: 24389511] |
| OVCAR-8 | IC50 |
10.5 μM
Compound: H-89
|
Antiproliferative activity against human OVCAR8 cells after 72 hrs by MTT assay
Antiproliferative activity against human OVCAR8 cells after 72 hrs by MTT assay
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[PMID: 24389511] |
| OVCAR-8 | IC50 |
10.5 μM
Compound: H-89
|
Cytotoxicity against human OVCAR8 cells assessed as growth inhibition after 72 hrs by MTT assay
Cytotoxicity against human OVCAR8 cells assessed as growth inhibition after 72 hrs by MTT assay
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[PMID: 24508830] |
| PC-3M | GI50 |
18 μM
Compound: H-89
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Inhibition of PC3M cells by SRB assay after 96 hrs
Inhibition of PC3M cells by SRB assay after 96 hrs
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[PMID: 17451235] |
| PC-3M | IC50 |
18 μM
Compound: H-89
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Growth inhibition of human PC3M cells by SRB assay
Growth inhibition of human PC3M cells by SRB assay
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[PMID: 18345609] |
| Sf9 | IC50 |
120 nM
Compound: H89
|
Inhibition of His-tagged human MSK1 expressed in Sf9 cells
Inhibition of His-tagged human MSK1 expressed in Sf9 cells
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[PMID: 10998351] |
| Sf9 | IC50 |
2.6 μM
Compound: H89
|
Inhibition of human PKBalpha expressed in SF9 cells
Inhibition of human PKBalpha expressed in SF9 cells
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[PMID: 10998351] |
| Sf9 | IC50 |
270 nM
Compound: H89
|
Inhibition of rat ROCK2 expressed in Sf9 cells
Inhibition of rat ROCK2 expressed in Sf9 cells
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[PMID: 10998351] |
| U-87MG ATCC | IC50 |
15 μM
Compound: H-89
|
Growth inhibition of human U87MG cells by SRB assay
Growth inhibition of human U87MG cells by SRB assay
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[PMID: 18345609] |
| U-87MG ATCC | IC50 |
15 μM
Compound: H-89
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Antiproliferative activity against human U87MG cells after 96 hrs by SRB assay
Antiproliferative activity against human U87MG cells after 96 hrs by SRB assay
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[PMID: 20151677] |
H-89 inhibits protein kinase A, in competitive fashion against ATP. H-89 causes a dose-dependent inhibition of the forskolin-induced protein phosphorylation, with no decrease in intracellular cyclic AMP levels in PC12D cells. H-89 significantly inhibits the forskolin-induced neurite outgrowth from PC12D cells. H-89 (30 μM) inhibits significantly cAMP-dependent histone IIb phosphorylation activity in PC12D cell lysates[1]. H-89 (1-2 μM) significantly slows the repriming rate in rat skinned fibres, most likely due to it deleteriously affecting the T-system potential. H-89 (10-100 μM) inhibits net Ca2+ uptake by the SR and affectes the Ca32-sensitivity of the contractile apparatus in rat skinned fibres[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 127243-85-0
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Appearance Solid
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Molecular Weight 446.36
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Formula C20H20BrN3O2S
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Color Off-white to light brown
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SMILES
O=S(C1=CC=CC2=C1C=CN=C2)(NCCNC/C=C/C3=CC=C(Br)C=C3)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (182)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
NPRC deletion mitigated atherosclerosis by inhibiting oxidative stress, inflammation and apoptosis in ApoE knockout mice. [Abstract]2023 Aug 9;8(1):290. PMID: 37553374 -
Cell Metab
Stress triggers irritable bowel syndrome with diarrhea through a spermidine-mediated decline in type I interferon. [Abstract]2025 Jan 7;37(1):87-103.e10. PMID: 39366386 -
Cell Metab
The thermogenic activity of adjacent adipocytes fuels the progression of ccRCC and compromises anti-tumor therapeutic efficacy. [Abstract]2021 Oct 5;33(10):2021-2039.e8. PMID: 34508696 -
Cell Metab
The phytochemical hyperforin triggers thermogenesis in adipose tissue via a Dlat-AMPK signaling axis to curb obesity. [Abstract]2021 Mar 2;33(3):565-580.e7. PMID: 33657393 -
Nat Immunol
2024 Nov;25(11):2057-2067. PMID: 39358444 -
Nat Metab
SLC6A6 imports taurine into mitochondria to sustain mitochondrial translation and tumour growth. [Abstract]2026 Mar;8(3):704-721. PMID: 41652173 -
Nat Metab
2024 Apr;6(4):708-723. PMID: 38499763 -
Ann Rheum Dis
2025 Oct 28:S0003-4967(25)04428-0. PMID: 41162285 -
Cell Mol Immunol
β-adrenoreceptor-triggered PKA activation negatively regulates the innate antiviral response. [Abstract]2023 Feb;20(2):175-188. PMID: 36600052
H-89 purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2023 Feb;20(2):175-188. [Abstract]
H89 (5 μM; 6 h) reverses ISO (Isoprenaline hydrochloride)-triggered decreases in the mRNA levels of the IFNB1 gene, the expression of which has been induced by SeV infection, in THP1 cells.
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Mol Cell
Histone phosphorylation integrates the hepatic glucagon-PKA-CREB gluconeogenesis program in response to fasting. [Abstract]2023 Apr 6;83(7):1093-1108.e8. PMID: 36863348 -
ACS Nano
Reduced Artifacts in Nanoscale Ratiometric Imaging and Sensing via Point-Spread Function Matching. [Abstract]2025 Jul 1;19(25):22755-22766. PMID: 40539535 -
Nat Commun
Protein kinase A is a dependent factor and therapeutic target in mouse models of fibrous dysplasia. [Abstract]2025 Jul 1;16(1):5425. PMID: 40593719 -
Nat Commun
2025 Jul 28;16(1):6942. PMID: 40721606 -
Nat Commun
An injectable liposome-anchored teriparatide incorporated gallic acid-grafted gelatin hydrogel for osteoarthritis treatment. [Abstract]2023 May 31;14(1):3159. PMID: 37258510 -
Nat Commun
2022 Nov 4;13(1):6648. PMID: 36333322 -
Acta Pharm Sin B
GRK2 mediated degradation of SAV1 initiates hyperplasia of fibroblast-like synoviocytes in rheumatoid arthritis. [Abstract]2024 Mar;14(3):1222-1240. PMID: 38486990 -
Autophagy
Impaired mitophagy contributes to osteogenesis and mineralization disorders in fibrous dysplasia. [Abstract]2026 Mar 15:1-22. PMID: 41803635 -
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Adv Sci (Weinh)
Osmotic Tension Asymmetry Drives Electrotactic Migration via PDLIM7-Polarized Microfilament Coordination in Breast Cancer Cells. [Abstract]2025 Dec 22:e15246. PMID: 41431193 -
Adv Sci (Weinh)
Calhm6 Governs Macrophage Polarization Through Chp1-Camk4-Creb1 Axis and Ectosomal Delivery in Inflammatory Responses. [Abstract]2025 Sep 26:e02395. PMID: 40999918 -
Adv Sci (Weinh)
Microbiota-Derived Inosine Suppresses Systemic Autoimmunity via Restriction of B Cell Differentiation and Migration. [Abstract]2025 Apr 28:e2409837. PMID: 40289872 -
Adv Sci (Weinh)
Targeting the E Prostanoid Receptor EP4 Mitigates Cardiac Fibrosis Induced by β-Adrenergic Activation. [Abstract]2025 Feb 7:e2413324. PMID: 39921269 -
Adv Sci (Weinh)
Granulosa Cell-Layer Stiffening Prevents Escape of Mural Granulosa Cells from the Post-Ovulatory Follicle. [Abstract]2024 Jul 1:e2403640. PMID: 38946588 -
Adv Sci (Weinh)
2021 Dec;8(24):e2100808. PMID: 34719888 -
Neuro Oncol
A disintegrin and metalloproteinase 22 activates integrin β1 through its disintegrin domain to promote the progression of pituitary adenoma. [Abstract]2024 Jan 5;26(1):137-152. PMID: 37555799 -
Theranostics
Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. [Abstract]2021 Mar 24;11(12):5650-5674. PMID: 33897873 -
Theranostics
CXCL12-mediated HOXB5 overexpression facilitates Colorectal Cancer metastasis through transactivating CXCR4 and ITGB3. [Abstract]2021 Jan 1;11(6):2612-2633. PMID: 33456563 -
J Adv Res
A novel TGR5 agonist Sauchinone ameliorates IMQ induced murine psoriasis by regulating macrophage polarization. [Abstract]2025 Apr 22:S2090-1232(25)00278-4. PMID: 40274226 -
Biomaterials
Self-amplified ROS production from fatty acid oxidation enhanced tumor immunotherapy by atorvastatin/PD-L1 siRNA lipopeptide nanoplexes. [Abstract]2022 Dec:291:121902. PMID: 36371945 -
J Exp Clin Cancer Res
GPR65 sensing tumor-derived lactate induces HMGB1 release from TAM via the cAMP/PKA/CREB pathway to promote glioma progression. [Abstract]2024 Apr 4;43(1):105. PMID: 38576043 -
J Exp Clin Cancer Res
Efficacy and mechanism of the combination of PARP and CDK4/6 inhibitors in the treatment of triple-negative breast cancer. [Abstract]2021 Apr 8;40(1):122. PMID: 33832512 -
Mater Today Bio
PKA activation rescues myocardial injury elicited by silica nanoparticles through improving oxidative stress, mitochondrial health, and copper homeostasis. [Abstract]2026 Mar 10:37:103021. PMID: 41890951 -
J Neuroinflammation
IL-1β+ tumor-associated macrophages accelerate glioblastoma progression by amplifying the PGE2-EP4 signaling. [Abstract]2025 Oct 14;22(1):233. PMID: 41088217 -
Int J Biol Sci
Chronic Stress-induced Serotonin Impairs Intestinal Epithelial Cell Mitochondrial Biogenesis via the AMPK-PGC-1α Axis. [Abstract]2024 Aug 19;20(11):4476-4495. PMID: 39247815 -
Int J Biol Sci
Chronic stress promotes colorectal cancer progression by enhancing glycolysis through β2-AR/CREB1 signal pathway. [Abstract]2023 Apr 2;19(7):2006-2019. PMID: 37151872
H-89 purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2023 Apr 2;19(7):2006-2019. [Abstract]
H-89 dihydrochloride (H-89; 10 μM; 2 h) blocks the epinephrine-induced phosphorylation of CREB1 in MC38 and HT-29 cells.
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Cell Death Dis
Activation of AKT via a dual mechanism enhances the susceptibility of melanoma cells to glucose deprivation. [Abstract]2025 Aug 7;16(1):595. PMID: 40774947 -
Cell Death Dis
S1PR3-driven positive feedback loop sustains STAT3 activation and keratinocyte hyperproliferation in psoriasis. [Abstract]2025 Jan 20;16(1):31. PMID: 39833165 -
Cell Death Dis
2024 Sep 30;15(9):699. PMID: 39349432 -
Genes Dis
2020 Nov 21;8(6):867-881. PMID: 34522714 -
NPJ Biofilms Microbiomes
Gut microbiota-mediated secondary bile acid alleviates Staphylococcus aureus-induced mastitis through the TGR5-cAMP-PKA-NF-κB/NLRP3 pathways in mice. [Abstract]2023 Feb 8;9(1):8. PMID: 36755021 -
Proc Natl Acad Sci U S A
A PKA-selective inhibitor captures an open but more ordered conformation of the PKA catalytic subunit. [Abstract]2026 May 12;123(19):e2536312123. PMID: 42096309 -
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Small Methods
An Integrated Microcurrent Delivery System Facilitates Human Parathyroid Hormone Delivery for Enhancing Osteoanabolic Effect. [Abstract]2025 Mar;9(3):e2401144. PMID: 39420694 -
Cell Commun Signal
Olanzapine suppresses mPFC activity-norepinephrine releasing to alleviate CLOCK-enhanced cancer stemness under chronic stress. [Abstract]2024 Jul 25;22(1):375. PMID: 39054537 -
Cell Commun Signal
Receptor activity-modifying protein 1 regulates mouse skin fibroblast proliferation via the Gαi3-PKA-CREB-YAP axis. [Abstract]2022 Apr 12;20(1):52. PMID: 35413847 -
Cell Commun Signal
Zinc-mediated activation of CREB pathway in proliferation of pulmonary artery smooth muscle cells in pulmonary hypertension. [Abstract]2021 Oct 11;19(1):103. PMID: 34635097 -
Int J Biol Macromol
Effect and mechanism of maternal prolactin levels on depressive-like behavior in prenatally stressed offspring. [Abstract]2026 Jan;340(Pt 1):150002. PMID: 41485664 -
Int J Biol Macromol
Genome-wide identification and expression analysis of neuropeptide G protein-coupled receptors in Urechis unicinctus (Annelida, Echiura). [Abstract]2025 Apr 26:143548. PMID: 40294673 -
Phytomedicine
Z-Ligustilide's antidepressant effects in adolescent depression involve the PTEN-induced putative kinase 1 (PINK1) - protein kinase A (PKA)- Na+/Ca2+ exchanger (NCLX) pathway, facilitating mitochondrial endosymbiosis and promoting mitophagy in microglia. [Abstract]2025 Dec:149:157494. PMID: 41218379 -
Phytomedicine
Shouhui Tongbian Capsule ameliorates obesity by enhancing energy consumption and promoting lipolysis via cAMP-PKA pathway. [Abstract]2025 Mar:138:156375. PMID: 39848021 -
Free Radic Biol Med
METTL3 deficiency leads to ovarian insufficiency due to IL-1β overexpression in theca cells. [Abstract]2024 May 31:222:72-84. PMID: 38825211 -
Sci Total Environ
Silica nanoparticles perturbed mitochondrial dynamics and induced myocardial apoptosis via PKA-DRP1-mitochondrial fission signaling. [Abstract]2022 Oct 10:842:156854. PMID: 35750168 -
Brain Behav Immun
NR2B-CaMKII signaling in the dentate gyrus driven by astrocytic P2Y1Rs mediates the antidepressant effect of low-dose LPS. [Abstract]2026 Mar 6:135:106529. PMID: 41796645 -
Brain Behav Immun
Dexamethasone attenuates neuropathic pain through spinal microglial expression of dynorphin A via the cAMP/PKA/p38 MAPK/CREB signaling pathway. [Abstract]2024 Jul:119:36-50. PMID: 38555991 -
Brain Behav Immun
Gut microbiota-mediated ursodeoxycholic acids regulate the inflammation of microglia through TGR5 signaling after MCAO. [Abstract]2024 Jan:115:667-679. PMID: 37989444 -
Anim Nutr
DHA induces adipocyte lipolysis through endoplasmic reticulum stress and the cAMP/PKA signaling pathway in grass carp (Ctenopharyngodon idella). [Abstract]2022 Dec 31:13:185-196. PMID: 37123617 -
Biomed Pharmacother
Teriparatide induces angiogenesis in ischemic cerebral infarction zones of rats through AC/PKA signaling and reduces ischemia-reperfusion injury. [Abstract]2022 Apr:148:112728. PMID: 35220030 -
Chem Sci
2019 Dec 6;11(4):1132-1139. PMID: 34084370 -
Stem Cell Res Ther
Mesenchymal stem cells combined with liraglutide relieve acute lung injury through apoptotic signaling restrained by PKA/β-catenin. [Abstract]2020 May 19;11(1):182. PMID: 32429994 -
Stem Cell Res Ther
Effect of hCMSCs and liraglutide combination in ALI through cAMP/PKAc/β-catenin signaling pathway. [Abstract]2020 Jan 3;11(1):2. PMID: 31900217 -
Aging Cell
nNOS-CAPON interaction mediates amyloid-β-induced neurotoxicity, especially in the early stages. [Abstract]2018 Jun;17(3):e12754. PMID: 29577585 -
Cell Death Discov
The LTB4-BLT1 axis attenuates influenza-induced lung inflammation by suppressing NLRP3 activation. [Abstract]2025 Apr 6;11(1):148. PMID: 40189592 -
Microbiol Res
The Arf-GAPs, AoAge1 and AoAge2, regulate diverse cellular processes, conidiation, trap formation, and secondary metabolism in Arthrobotrys oligospora. [Abstract]2024 May 24:285:127779. PMID: 38810485 -
Cell Rep
2022 Oct 18;41(3):111498. PMID: 36261001 -
Cell Rep
Gut microbiota-mediated secondary bile acids regulate dendritic cells to attenuate autoimmune uveitis through TGR5 signaling. [Abstract]2021 Sep 21;36(12):109726. PMID: 34551302 -
J Med Chem
Discovery of Novel P2Y14R Inhibitors for Ameliorating Liver Fibrosis by Suppressing Hepatic Stellate Cell Activation. [Abstract]2025 Nov 13;68(21):23277-23299. PMID: 41178760 -
Clin Transl Med
Activation of Toll-like receptor 7 provides cardioprotection in septic cardiomyopathy-induced systolic dysfunction. [Abstract]2021 Jan;11(1):e266. PMID: 33463061 -
Sci Signal
Airway relaxation mechanisms and structural basis of osthole for improving lung function in asthma. [Abstract]2020 Nov 24;13(659):eaax0273. PMID: 33234690 -
Elife
2023 Jan 16:12:e81438. PMID: 36645741 -
Environ Health (Wash)
Effects on Synaptic Plasticity Markers in Fetal Mice and HT22 Neurons upon F-53B Exposure: The Role of PKA Cytoplasmic Retention. [Abstract]2024 Aug 16;2(11):776-785. PMID: 39568694 -
Phytother Res
Protective effects of 4-geranyloxy-2,6-dihydroxybenzophenonel on DSS-induced ulcerative colitis in mice via regulation of cAMP/PKA/CREB and NF-κB signaling pathways. [Abstract]2023 Apr;37(4):1330-1345. PMID: 36428266 -
Transl Psychiatry
TGR5 dysfunction underlies chronic social defeat stress via cAMP/PKA signaling pathway in the hippocampus. [Abstract]2025 Oct 6;15(1):366. PMID: 41053031 -
J Agric Food Chem
Sargassum fusiforme Fucoidan Ameliorates Obesity-Associated Metabolic Dysfunction via a Tauroursodeoxycholic Acid-Mediated TGR5-cAMP-PKA Signaling Pathway. [Abstract]2025 Aug 13;73(32):20235-20253. PMID: 40758868 -
J Agric Food Chem
Sesquiterpenoids from Alpinia oxyphylla with GLP-1 Stimulative Effects through Ca2+/CaMKII and PKA Pathways and Multiple-Enzyme Inhibition. [Abstract]2023 Nov 1;71(43):16148-16159. PMID: 37871265 -
Prog Neurobiol
2021 Jul:202:102041. PMID: 33766679 -
Ecotoxicol Environ Saf
Role of PKA/CREB/BDNF signaling in PM2.5-induced neurodevelopmental damage to the hippocampal neurons of rats. [Abstract]2021 May:214:112005. PMID: 33640725 -
Cancer Cell Int
Phenyllactic acid promotes cell migration and invasion in cervical cancer via IKK/NF-κB-mediated MMP-9 activation. [Abstract]2019 Sep 23;19:241. PMID: 31572058 -
J Bone Miner Res
Lgr4 promotes aerobic glycolysis and differentiation in osteoblasts via the canonical Wnt/β-catenin pathway. [Abstract]2021 Aug;36(8):1605-1620. PMID: 33950533 -
Chin Med
Electroacupuncture promotes BDNF-dependent neurogenesis via microglial reprogramming in a chronic stress model. [Abstract]2026 Feb 3;21(1):62. PMID: 41634791 -
Biochem Pharmacol
Calcitonin gene-related peptide/protein kinase A: A novel pathway to regulate patency of ductus arteriosus. [Abstract]2026 Jun:248:117840. PMID: 41724283 -
Chem Biol Interact
Oxyphylla A exerts antiparkinsonian effects by ameliorating 6-OHDA-induced mitochondrial dysfunction and dyskinesia in vitro and in vivo. [Abstract]2024 Sep 2:111224. PMID: 39233265 -
Nutr Diabetes
Intrauterine hyperglycaemia during late gestation caused mitochondrial dysfunction in skeletal muscle of male offspring through CREB/PGC1A signaling. [Abstract]2024 Jul 23;14(1):56. PMID: 39043630 -
Cells
Glucagon Promotes Gluconeogenesis through the GCGR/PKA/CREB/PGC-1α Pathway in Hepatocytes of the Japanese Flounder Paralichthys olivaceus. [Abstract]2023 Apr 6;12(7):1098. PMID: 37048171 -
Life Sci
Mechanistic insights into PPARγ's role in suppressing collagen-driven platelet activation. [Abstract]2026 Jan 15:385:124138. PMID: 41354414 -
Glia
RetSat Knockout Mitigates Hypoxia-Induced Microglial Activation by Enhancing Lipid Droplets Degradation. [Abstract]2026 Feb;74(2):e70118. PMID: 41388745 -
Life Sci
Polymyxin B induces pigmentation by upregulating ATG2A-ERK/CREB-MITF-PMEL17 signaling axis. [Abstract]2025 May 15:369:123553. PMID: 40074142 -
Life Sci
Genistein accelerates glucose catabolism via activation the GPER-mediated cAMP/PKA-AMPK signaling pathway in broiler chickens. [Abstract]2022 Aug 15:303:120676. PMID: 35640778 -
CNS Neurosci Ther
Inhibition of ADORA3 promotes microglial phagocytosis and alleviates chronic ischemic white matter injury. [Abstract]2024 May;30(5):e14742. PMID: 38715283 -
Nutrients
2020 Jan 26;12(2):329. PMID: 31991934 -
Int J Mol Sci
Alkaline Dilution Alters Sperm Motility in Dairy Goat by Affecting sAC/cAMP/PKA Pathway Activity. [Abstract]2023 Jan 16;24(2):1771. PMID: 36675287 -
Int J Mol Sci
Salicylate Sodium Suppresses Monocyte Chemoattractant Protein-1 Production by Directly Inhibiting Phosphodiesterase 3B in TNF-α-Stimulated Adipocytes. [Abstract]2022 Dec 24;24(1):320. PMID: 36613764 -
Int J Mol Sci
Asprosin in the Paraventricular Nucleus Induces Sympathetic Activation and Pressor Responses via cAMP-Dependent ROS Production. [Abstract]2022 Oct 20;23(20):12595. PMID: 36293450 -
Int J Mol Sci
AWRK6, a Novel GLP-1 Receptor Agonist, Attenuates Diabetes by Stimulating Insulin Secretion. [Abstract]2018 Oct 7;19(10). pii: E3053. PMID: 30301245 -
Front Pharmacol
Simvastatin Enhances Activity and Trafficking of α7 Nicotinic Acetylcholine Receptor in Hippocampal Neurons Through PKC and CaMKII Signaling Pathways. [Abstract]2018 Apr 12:9:362. PMID: 29706890 -
Invest Ophthalmol Vis Sci
Epac1 Inhibits Orbital Fibroblast Activation to Ameliorate Thyroid-Associated Orbitopathy-Like Features Through the JAK/STAT Signaling Pathway. [Abstract]2025 Jul 1;66(9):68. PMID: 40736178 -
Int Immunopharmacol
Phenylacetaldehyde attenuates Cutibacterium acnes-induced inflammation in keratinocytes and monocytes. [Abstract]2025 May 17:158:114885. PMID: 40383096 -
Eur J Pharmacol
Exendin-4, a glucagon-like peptide-1 receptor agonist, regulates ductus arteriosus by vasodilation and anti-remodeling through the PKA pathway. [Abstract]2024 Nov 6:985:177106. PMID: 39515563 -
Am J Physiol Cell Physiol
2024 Jun 1;326(6):C1683-C1696. PMID: 38646785 -
Int Immunopharmacol
Hypersampsonone H attenuates ulcerative colitis via inhibition of PDE4 and regulation of cAMP/PKA/CREB signaling pathway. [Abstract]2024 Feb 15:128:111490. PMID: 38218008 -
BMC Biol
GnRH-driven FTO-mediated RNA m6A modification promotes gonadotropin synthesis and secretion. [Abstract]2024 May 3;22(1):104. PMID: 38702712 -
Immunotargets Ther
Myeloid Cell-Derived Thrombospondin-1 Mediates Anti-Metastatic Effects of Histamine Dihydrochloride in B16 Melanoma. [Abstract]2026 Jun 6:15:596703. PMID: 42281617 -
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Mol Neurobiol
Gastrodin Ameliorates Post-Stroke Depressive-Like Behaviors Through Cannabinoid-1 Receptor-Dependent PKA/RhoA Signaling Pathway. [Abstract]2025 Jan;62(1):366-385. PMID: 38856794 -
Poult Sci
PKA and PKC signaling pathways mediate vitamin D₃-regulated intestinal phosphorus absorption in broiler chickens. [Abstract]2026 Mar 12;105(6):106762. PMID: 41855802 -
J Cell Mol Med
Effect of Curcuma longa extract on reproduction function in mice and testosterone production in Leydig cells. [Abstract]2024 Apr;28(8):e18303. PMID: 38613362 -
FASEB J
Morinda officinalis oligosaccharides ameliorate depressive-like behaviors in poststroke rats through upregulating GLUT3 to improve synaptic activity. [Abstract]2020 Oct;34(10):13376-13395. PMID: 32812265 -
J Cell Mol Med
Maresin Conjugates in Tissue Regeneration 1 improves alveolar fluid clearance by up-regulating alveolar ENaC, Na, K-ATPase in lipopolysaccharide-induced acute lung injury. [Abstract]2020 Apr;24(8):4736-4747. PMID: 32160403 -
J Lipid Res
2026 Feb;67(2):100981. PMID: 41565113 -
iScience
The matricellular protein Fibulin-5 regulates β-cell proliferation in an autocrine/paracrine manner. [Abstract]2025 Jan 21;28(2):111856. PMID: 39995864 -
iScience
Pleiotropic roles of Ras GTPases in the nematode-trapping fungus Arthrobotrys oligospora identified through multi-omics analyses. [Abstract]2021 Jul 8;24(8):102820. PMID: 34337364 -
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J Neurosci
Olig2-Targeted G-Protein-Coupled Receptor Gpr17 Regulates Oligodendrocyte Survival in Response to Lysolecithin-Induced Demyelination. [Abstract]2016 Oct 12;36(41):10560-10573. PMID: 27733608
H-89 purchased from MedChemExpress. Usage Cited in: J Neurosci. 2016 Oct 12;36(41):10560-10573. [Abstract]
The inhibition of PKA signaling with PKA inhibitor H89 reduces cell viability observed after Pranlukast treatment.
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Sci Rep
2025 Oct 16;15(1):36270. PMID: 41102391 -
J Biol Chem
HIV-1 exposure promotes PKG1-mediated phosphorylation and degradation of stathmin to increase epithelial barrier permeability. [Abstract]2021 Jan-Jun:296:100644. PMID: 33839152 -
Aging (Albany NY)
Fibroblast growth factor 2 contributes to the effect of salidroside on dendritic and synaptic plasticity after cerebral ischemia/reperfusion injury. [Abstract]2020 Jun 9;12(11):10951-10968. PMID: 32518214 -
Aging (Albany NY)
Heme oxygenase-1 ameliorates oxidative stress-induced endothelial senescence via regulating endothelial nitric oxide synthase activation and coupling. [Abstract]2018 Jul 24;10(7):1722-1744. PMID: 30048241
H-89 purchased from MedChemExpress. Usage Cited in: Aging (Albany NY). 2018 Jul 24;10(7):1722-1744. [Abstract]
Expressions of p-eNOS (Ser1177) and total eNOS are measured in HUVECs treated with or without H2O2 and Hemin in the presence or absence of Akt inhibitor MK-2206, PKA inhibitor H-89 or AMPK inhibitor Compound C (CP-C).
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J Periodontol
Intermittent parathyroid hormone promotes cementogenesis in a PKA- and ERK1/2-dependent manner. [Abstract]2019 Sep;90(9):1002-1013. PMID: 31026057 -
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BMC Genomics
Identification of the principal neuropeptide MIP and its action pathway in larval settlement of the echiuran worm Urechis unicinctus. [Abstract]2024 Apr 3;25(1):337. PMID: 38641568 -
Heliyon
CD38 symmetric dimethyl site R58 promotes malignant tumor cell immune escape by regulating the cAMP-GSK3β-PD-L1 axis. [Abstract]2024 Sep 19;10(19):e37958. PMID: 39386836 -
Biol Trace Elem Res
2021 Jul;199(7):2687-2694. PMID: 32926327 -
Psychoneuroendocrinology
2020 Jul;117:104680. PMID: 32387876 -
Bone
hPTH(3-34)(29-34) selectively activated PKC and mimicked osteoanabolic effects of hPTH(1-34). [Abstract]2020 Jun;135:115326. PMID: 32200023 -
Exp Cell Res
Down-regulation of A3AR signaling by IL-6-induced GRK2 activation contributes to Th17 cell differentiation. [Abstract]2021 Feb 15;399(2):112482. PMID: 33434531 -
J Gastroenterol Hepatol
Targeting DRP1Ser637 Phosphorylation Alleviates Acidic Bile Salt-Induced NF-κB Activation via Mitochondrial Protection. [Abstract]2025 Jul 31. PMID: 40741897 -
Andrology
Ubiquitin-mediated immunoglobulin-binding protein 1 degradation promotes autophagy and sperm capacitation in vitro. [Abstract]2025 Jul 2. PMID: 40600667 -
Acta Biochim Biophys Sin (Shanghai)
Evolution of GCGR family ligand-receptor extensive cross-interaction systems suggests a therapeutic direction for hyperglycemia in mammals. [Abstract]2023 Nov 15;55(12):1855-1863. PMID: 37969012 -
Front Physiol
Endoplasmic Reticulum Stress/Ca2+-Calmodulin-Dependent Protein Kinase/Signal Transducer and Activator of Transcription 3 Pathway Plays a Role in the Regulation of Cellular Zinc Deficiency in Myocardial Ischemia/Reperfusion Injury. [Abstract]2022 Jan 5:12:736920. PMID: 35069232 -
Front Physiol
Roles of Endothelial Motilin Receptor and Its Signal Transduction Pathway in Motilin-Induced Left Gastric Artery Relaxation in Dogs. [Abstract]2021 Oct 28;12:770430. PMID: 34777026 -
Endocrinology
2023 Mar 13;164(5):bqad042. PMID: 36929849 -
Front Oncol
SUZ12 Loss Amplifies the Ras/ERK Pathway by Activating Adenylate Cyclase 1 in NF1-Associated Neurofibromas. [Abstract]2021 Oct 6;11:738300. PMID: 34692515 -
Korean J Pain
Perampanel ameliorates nitroglycerin-induced migraine through inhibition of the cAMP/PKA/CREB signaling pathway in the trigeminal ganglion in rats. [Abstract]2023 Jul 1;36(3):335-346. PMID: 37394274 -
Biol Reprod
Activation of cAMP/PKA and ERK signaling pathways and potential role of Phoenixin in the reproductive axis of spotted scat (Scatophagus argus)†. [Abstract]2025 Sep 18:ioaf213. PMID: 40973150 -
Mol Reprod Dev
CD55 is upregulated by cAMP/PKA/AKT and modulates human decidualization via Src and ERK pathway and decidualization-related genes. [Abstract]2022 May;89(5-6):256-268. PMID: 35474595 -
Arch Biochem Biophys
Identification and characterization of human PEIG-1/GPRC5A as a 12-O-tetradecanoyl phorbol-13-acetate (TPA) and PKC-induced gene. [Abstract]2020 Jul 15;687:108375. PMID: 32339486 -
Peptides
2026 Jan:195:171463. PMID: 41485715 -
Peptides
Neuropeptide Y promotes hepatic apolipoprotein A1 synthesis and secretion through neuropeptide Y Y5 receptor. [Abstract]2022 Aug:154:170824. PMID: 35660638 -
Toxicol Lett
Adenosine receptor A2B mediates alcoholic hepatitis by regulating cAMP levels and the NF-KB pathway. [Abstract]2022 Apr 15:359:84-95. PMID: 35143882 -
Mol Brain
Meningitic Escherichia coli α-hemolysin aggravates blood-brain barrier disruption via targeting TGFβ1-triggered hedgehog signaling. [Abstract]2021 Jul 19;14(1):116. PMID: 34281571 -
Neurogastroenterol Motil
Acute stress induces visceral hypersensitivity via glucocorticoid receptor-mediated membrane insertion of TRPM8: Involvement of a non-receptor tyrosine kinase Pyk2. [Abstract]2020 Oct;32(10):1514-1528. PMID: 32391653 -
Int J Biochem Cell Biol
Downregulation of Pannexin-1 attenuates sepsis-induced acute lung injury via activation of the cAMP/PKA pathway. [Abstract]2026 Jun-Jul:195-196:106941. PMID: 41850417 -
J Orthop Surg Res
ITGB1 alleviates osteoarthritis by inhibiting cartilage inflammation and apoptosis via activating cAMP pathway. [Abstract]2023 Nov 8;18(1):849. PMID: 37941009 -
Infect Immun
Phosphothreonine Lyase Promotes p65 Degradation in a Mitogen-Activated Protein Kinase/Mitogen- and Stress-Activated Protein Kinase 1-Dependent Manner. [Abstract]2018 Dec 19;87(1). pii: e00508-1 PMID: 30396897
H-89 purchased from MedChemExpress. Usage Cited in: Infect Immun. 2018 Dec 19;87(1). pii: e00508-1 [Abstract]
J774A.1 cells are pre-treated with 473 U0126 (10 μM), SB208530 (10 μM), SP600125 (10 μM), or H89 (50 μM), then infected with ΔeseH E. piscicida at the MOI of 10 for 3.5 h. Cell lysates were probed with anti-phospho-p65 (Ser276), anti-p65, and anti-β-actin antibodies.
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BMC Pharmacol Toxicol
Bisdemethoxycurcumin alleviates LPS-induced acute lung injury via activating AMPKα pathway. [Abstract]2023 Nov 20;24(1):63. PMID: 37986186 -
Vet Microbiol
Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) induces IL-6 production through TAK-1/JNK/AP-1 and TAK-1/NF-κB signaling pathways. [Abstract]2021 May:256:109061. PMID: 33836390 -
Fitoterapia
Oligostilbenes from the seeds of Paeonia lactiflora as potent GLP-1 secretagogues targeting TGR5 receptor. [Abstract]2022 Nov:163:105336. PMID: 36257378 -
Reprod Sci
Role of the Glycogen Synthase Kinase 3-Cyclic AMP/Protein Kinase A in the Immobilization of Human Sperm by Tideglusib. [Abstract]2023 Apr;30(4):1281-1290. PMID: 36207578 -
Eur J Neurosci
2025 Jul;62(2):e70198. PMID: 40673719 -
PeerJ
Icariside II induces rapid phosphorylation of endothelial nitric oxide synthase via multiple signaling pathways. [Abstract]2022 Oct 25:10:e14192. PMID: 36312762 -
Purinergic Signal
Lysophosphatidylcholine induces adenosine release from macrophages via TRPM7-mediated mitochondrial activation. [Abstract]2022 Sep;18(3):317-343. PMID: 35779163 -
Exp Ther Med
Melatonin alleviates traumatic brain injury-induced anxiety-like behaviors in rats: Roles of the protein kinase A/cAMP-response element binding signaling pathway. [Abstract]2022 Apr;23(4):248. PMID: 35261620 -
Biochem Biophys Res Commun
Olodaterol promotes thermogenesis in brown adipocytes via regulation of the β2-AR/cAMP/PKA signaling pathway. [Abstract]2024 Apr 9:703:149689. PMID: 38382361 -
J Bone Miner Metab
2021 Sep;39(5):748-756. PMID: 33829324 -
Eur J Histochem
Overexpression of GPER1 suppressed esophageal carcinoma growth via activating cAMP pathway. [Abstract]2026 Jan 26;70(1). PMID: 41586754 -
J Neurophysiol
cAMP-dependent protein kinase signaling is required for ( 2R,6R)-hydroxynorketamine to potentiate hippocampal glutamatergic transmission. [Abstract]2024 Jan 1;131(1):64-74. PMID: 38050689 -
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Comp Biochem Physiol B Biochem Mol Biol
Molecular and functional characterization of the kisspeptin receptor Kissr3 in the large yellow croaker (Larimichthys crocea). [Abstract]2026 Jun-Jul:284:111225. PMID: 41912081 -
Gen Comp Endocrinol
Characterization of serotonin receptors Aj5-HTR2 and Aj5-HTR7 reveals potential roles in circadian regulation of Apostichopus japonicus. [Abstract]2026 Mar:379:114913. PMID: 41819218 -
Biol Pharm Bull
Adenosine Stimulates Beating of Neonatal Brain-Derived Cilia through Adenosine A2B Receptor on the Cilia and Activation of Protein Kinase A Pathway. [Abstract]2024;47(6):1113-1118. PMID: 38839362 -
Proteome Sci
Proteomic analysis of neonatal mouse hearts shows PKA functions as a cardiomyocyte replication regulator. [Abstract]2023 Oct 11;21(1):16. PMID: 37821903 -
J Int Med Res
Progesterone activates the cyclic AMP-protein kinase A signalling pathway by upregulating ABHD2 in fertile men. [Abstract]2021 Mar;49(3):300060521999527. PMID: 33752482 -
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bioRxiv
Rapid Hippocampal Synaptic Potentiation Induced by Ketamine Metabolite ( 2R , 6R )-Hydroxynorketamine Persistently Primes Synaptic Plasticity. [Abstract]2024 Oct 22:2024.10.18.619152. PMID: 39484512 -
bioRxiv
Enhanced kinase translocation reporters for simultaneous real-time measurement of PKA, ERK, and Ca2. [Abstract]2024 Oct 2:2024.09.30.615856. PMID: 39411162 -
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Evid Based Complement Alternat Med
The Inhibition Effects of Shenmai Injection on Acetylcholine-Induced Catecholamine Synthesis and Secretion by Modulating Nicotinic Acetylcholine Receptor Ion Channels in Cultured Bovine Adrenal Medullary Cells. [Abstract]2020 Dec 15:2020:8514926. PMID: 33456492 -
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Solvent & Solubility
DMSO : 100 mg/mL (224.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.60 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (5.60 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
Kinase activities are assayed at 30°C for 2-5 min by measuring the transfer of 32P from [γ-32P]ATP to substrates. The reaction is terminated by adding 1 mL of 20% trichloroacetic acid, following the addition of 100 μg of bovine serum albumin as a carrier protein. The sample is centrifuged at 3000 rpm for 10 min, the pellet is resuspended in 5% trichloroacetic acid solution, the final pellet is dissolved in 1 mL of 1 N NaOH and the radioactivity is measured in a liquid scintillation counter.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
After 48 h in culture, PCl2D cells are cultured in test medium containing 30 μM H-89 for 1 h and then exposed to fresh medium that contained both 10 μM forskolin and 30 μM H-89. Cells are scraped off with a rubber policeman and sonicated in the presence of 0.5 mL of 6% trichloroacetic acid. To extract trichloroacetic acid, 2 mL of petroleum ether is added, the preparation mixed and centrifuged at 3000 rpm for 10 min. After aspiration of the upper layer, the residue sample solution is used for determination.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Male albino mice weighing 20-25 g are obtained. Pentoxifylline (25, 50, 100 mg/kg), bucladesine (50, 100, 300 nM/mouse) and H-89 (0.05, 0.1, 0.2 mg/100 g) are administered intraperitoneally (i.p.) 30 min before intravenous (i.v.) infusion of PTZ. In combination groups, the first and second components are injected 45 and 30 min before PTZ infusion. In all groups, the respective control animalsreceive an appropriate volume of vehicle. For the i.v. infusion, the needle is inserted into the lateral tail vein, fixed to the tail vein by a narrow piece of adhesive tape, and the animal is allowed to move freely. PTZ solution is infused at a concentration rate of 1 mL/min.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Chijiwa T, et al. Inhibition of forskolin-induced neurite outgrowth and protein phosphorylation by a newly synthesized selective inhibitor of cyclic AMP-dependent protein kinase, N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H-89), of PC12D pheochromocytoma cells. J Biol Chem. 1990 Mar 25;265(9):5267-72. [Content Brief]
[2]. Blazev R, et al. Effects of the PKA inhibitor H-89 on excitation-contraction coupling in skinned and intact skeletal muscle fibres. J Muscle Res Cell Motil. 2001;22(3):277-86. [Content Brief]
[3]. Hosseini-Zare MS, et al. Effects of pentoxifylline and H-89 on epileptogenic activity of bucladesine in pentylenetetrazol-treated mice. Eur J Pharmacol. 2011 Nov 30;670(2-3):464-70. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2403 mL | 11.2017 mL | 22.4034 mL | 56.0086 mL |
| 5 mM | 0.4481 mL | 2.2403 mL | 4.4807 mL | 11.2017 mL | |
| 10 mM | 0.2240 mL | 1.1202 mL | 2.2403 mL | 5.6009 mL | |
| 15 mM | 0.1494 mL | 0.7468 mL | 1.4936 mL | 3.7339 mL | |
| 20 mM | 0.1120 mL | 0.5601 mL | 1.1202 mL | 2.8004 mL | |
| 25 mM | 0.0896 mL | 0.4481 mL | 0.8961 mL | 2.2403 mL | |
| 30 mM | 0.0747 mL | 0.3734 mL | 0.7468 mL | 1.8670 mL | |
| 40 mM | 0.0560 mL | 0.2800 mL | 0.5601 mL | 1.4002 mL | |
| 50 mM | 0.0448 mL | 0.2240 mL | 0.4481 mL | 1.1202 mL | |
| 60 mM | 0.0373 mL | 0.1867 mL | 0.3734 mL | 0.9335 mL | |
| 80 mM | 0.0280 mL | 0.1400 mL | 0.2800 mL | 0.7001 mL | |
| 100 mM | 0.0224 mL | 0.1120 mL | 0.2240 mL | 0.5601 mL |
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