2. Academic Validation
  3. PVT1 alleviates hypoxia-induced endothelial apoptosis by enhancing autophagy via the miR-15b-5p/ATG14 and miR-424-5p/ATG14 axis

PVT1 alleviates hypoxia-induced endothelial apoptosis by enhancing autophagy via the miR-15b-5p/ATG14 and miR-424-5p/ATG14 axis

  • Biochem Biophys Res Commun. 2023 Sep 3:671:1-9. doi: 10.1016/j.bbrc.2023.06.001.
Ping Zhang 1,  Shenghui Gong 2,  Shuoshuo Li 3,  Zengqiang Yuan 4
Affiliations

Affiliations

  • 1 Hengyang Medical College, University of South China, 421001, Hengyang, Hunan, China; The Brain Science Center, Beijing Institute of Basic Medical Sciences, 100850, Beijing, China.
  • 2 The Brain Science Center, Beijing Institute of Basic Medical Sciences, 100850, Beijing, China.
  • 3 The Brain Science Center, Beijing Institute of Basic Medical Sciences, 100850, Beijing, China; School of Life Science, Beijing University of Chinese Medicine, 100105, Beijing, China. Electronic address: [email protected].
  • 4 The Brain Science Center, Beijing Institute of Basic Medical Sciences, 100850, Beijing, China. Electronic address: [email protected].
PMID: 37290278 DOI: 10.1016/j.bbrc.2023.06.001
Abstract

Endothelial dysfunction plays a crucial role in the pathogenesis of vascular disease. Long noncoding RNA (lncRNA) and MicroRNA (miRNA) play important roles in various cellular processes and are involved in several vascular endothelial cells (VECs) biological processes, including cell growth, migration, Autophagy, and Apoptosis. The functions of plasmacytoma variant translocation 1 (PVT1) in VECs have been progressively investigated in recent years, mainly with regard to proliferation and migration of endothelial cells (ECs). However, the mechanism underlying the regulation of Autophagy and Apoptosis in human umbilical vein endothelial cells (HUVEC) by PVT1 remains unclear. The present study showed that PVT1 knockdown accelerated Apoptosis induced by oxygen and glucose deprivation (OGD) through suppression of cellular Autophagy. Bioinformatic prediction of PVT1 target miRNAs revealed that PVT1 interacts with miR-15b-5p and miR-424-5p. The study further showed that miR-15b-5p and miR-424-5p inhibit the functions of Autophagy related 14 (ATG14) and suppress cellular Autophagy. The results showed that PVT1 can function as a competing endogenous RNA (ceRNA) of miR-15b-5p and miR-424-5p and promote cellular Autophagy by competitive binding, which down-regulates Apoptosis. The results showed that PVT1 can function as a competing endogenous RNA (ceRNA) of miR-15b-5p and miR-424-5p and promote cellular Autophagy through competitive binding, which down-regulates Apoptosis. The study provides insight into a novel therapeutic target that may be explored in the future for the treatment of Cardiovascular Disease.

Keywords

Apoptosis; Autophagy; Endothelial injury; ceRNA; lncRNA.

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