2. Academic Validation
  3. Directed Differentiation of Human iPSCs into Microglia-Like Cells Using Defined Transcription Factors

Directed Differentiation of Human iPSCs into Microglia-Like Cells Using Defined Transcription Factors

  • Methods Mol Biol. 2023;2683:53-68. doi: 10.1007/978-1-0716-3287-1_5.
Shih-Wei Chen 1,  Yu-Hui Wong 2
Affiliations

Affiliations

  • 1 Department of Life Sciences and Institute of Genome Sciences, College of Life Sciences, National Yang Ming Chiao Tung University, Taipei, Taiwan.
  • 2 Brain Research Center, National Yang Ming Chiao Tung University, Taipei, Taiwan. [email protected].
PMID: 37300766 DOI: 10.1007/978-1-0716-3287-1_5
Abstract

The generation of a homogeneous population of microglia from human induced pluripotent stem cells (hiPSCs) is crucial to modeling neurological disorders, as well as the carrying out of drug screening and toxicity testing. Here, we provide a stepwise protocol for the simple, robust, and efficient differentiation of hiPSCs into microglia-like cells (iMGs) by overexpression of SPI1 and CEBPA. This protocol details hiPSC culture, lentivirus production, lentivirus delivery, and, finally, the differentiation and validation of the iMG cells.

Keywords

CEBPA; Induced pluripotent stem cells; Microglia; SPI1; Transcription factor-based differentiation.

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