2. Academic Validation
  3. Polyoxazoline-conjugated porcine serum albumin as an artificial plasma expander for dogs

Polyoxazoline-conjugated porcine serum albumin as an artificial plasma expander for dogs

  • Sci Rep. 2023 Jun 14;13(1):9512. doi: 10.1038/s41598-023-35999-4.
Wataru Okamoto 1,  Tomone Usui 1,  Mai Hasegawa 1,  Tatsuhiro Kobayashi 1,  Junya Fujisawa 1,  Kazuaki Taguchi 2,  Kazuaki Matsumoto 2,  Mitsutomo Kohno 3,  Masayuki Iwazaki 4,  Shotaro Shimano 5,  Itsuma Nagao 5,  Hiroto Toyoda 5,  Naoki Matsumura 5,  Hirotaka Tomiyasu 5,  Ryota Tochinai 6,  Teruyuki Komatsu 7
Affiliations

Affiliations

  • 1 Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo, 112-8551, Japan.
  • 2 Division of Pharmacodynamics, Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo, 105-8512, Japan.
  • 3 Department of General Thoracic Surgery, Saitama Medical Center, Saitama Medical University, 1981 Kamoda, Kawagoe-shi, Saitama, 350-8550, Japan.
  • 4 Department of Thoracic Surgery, School of Medicine, Tokai University, 143 Shimokasuya, Isehara-shi, Kanagawa, 259-1193, Japan.
  • 5 Department of Veterinary Internal Medicine, Graduate School of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
  • 6 Department of Veterinary Pathophysiology and Animal Health, Graduate School of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
  • 7 Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo, 112-8551, Japan. [email protected].
PMID: 37316550 DOI: 10.1038/s41598-023-35999-4
Abstract

Veterinary medicine has made tremendous progress for domestic dogs, which are irreplaceable family members enriching human life. Nevertheless, no adequate supply system exists for their blood products. This study examined the synthesis, structure, safety, and efficacy of poly(2-ethyl-2-oxazoline)-conjugated porcine serum albumin (POx-PSA) as an artificial plasma expander for dogs. The aqueous POx-PSA solution showed moderately high colloid osmotic pressure and good blood cell compatibility. Actually, lyophilized powder stored for 1 year can regenerate into a homogeneous solution. The circulation half-life of POx-PSA in rats was 2.1-fold longer than that of naked PSA. Rats produced neither anti-PSA IgG antibody nor anti-POx IgG antibody, which suggests excellent immunological stealth properties of POx-PSA. Complete resuscitation of hemorrhagic shock in rats was achieved soon after injection of POx-PSA solution. Serum biochemistry tests and histopathological observations indicated no abnormality in the related organs. When POx-PSA was administered to dogs intravenously, (i) no serum biochemical or hematological alteration was observed, also (ii) no overt deterioration of animal health was observed. These results indicate that POx-PSA has potential as an artificial plasma expander for dogs.

Figures
Products