2. Academic Validation
  3. Jujuboside B suppresses angiogenesis and tumor growth via blocking VEGFR2 signaling pathway

Jujuboside B suppresses angiogenesis and tumor growth via blocking VEGFR2 signaling pathway

  • Heliyon. 2023 Jun 7;9(6):e17072. doi: 10.1016/j.heliyon.2023.e17072.
Pan Zhang 1,  Xing Lai 2,  Mao-Hua Zhu 2,  Jiangpei Shi 2,  Hong Pan 2 3,  Yanhu Huang 2,  Run-Jie Guo 1,  Qin Lu 2,  Chao Fang 2 3,  Mei Zhao 1
Affiliations

Affiliations

  • 1 Department of Pharmacy, Shanghai University of Medicine & Health Sciences, Shanghai, 201318, China.
  • 2 Hongqiao International Institute of Medicine, Tongren Hospital and State Key Laboratory of Systems Medicine for Cancer, Department of Pharmacology and Chemical Biology, Shanghai Jiao Tong University School of Medicine (SJTU-SM), Shanghai, 200025, China.
  • 3 Key Laboratory of Basic Pharmacology of Ministry of Education & Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, 563003, China.
PMID: 37484305 DOI: 10.1016/j.heliyon.2023.e17072
Abstract

Jujuboside B (JuB), one of the main active triterpenoid saponins from the traditional Chinese medicine Ziziphus jujuba, possesses a wide range of pharmacological activities. However, it is unknown whether JuB can inhibit tumor angiogenesis, a crucial step in solid tumor growth. In this study, we found that JuB significantly inhibited the proliferation, migration, and tube formation of human umbilical vein endothelial cells in a dose-dependent manner. JuB also suppressed angiogenesis in chick embryo chorioallantoic membranes and Matrigel plugs. Moreover, through angiogenesis inhibition, JuB delayed the growth of human HCT-15 colorectal Cancer xenograft in mice. Western blot assay demonstrated that JuB inhibited the phosphorylation of VEGFR2 and its key downstream protein kinases, such as Akt, FAK, Src, and PLCγ1. In conclusion, the antiangiogenic potency and molecular mechanism of JuB are revealed for the first time, indicating that this triterpene saponin may be further explored as a potential drug candidate or lead compound for antiangiogenic Cancer therapy.

Keywords

Antiangiogenesis; Chick embryo chorioallantoic membrane; Jujuboside B; Triterpenoid saponin; VEGFR2.

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