2. Academic Validation
  3. D-tartaric acid doping improves the performance of whole-cell bacteria imprinted polymer for sensing Vibrio parahaemolyticus

D-tartaric acid doping improves the performance of whole-cell bacteria imprinted polymer for sensing Vibrio parahaemolyticus

  • Anal Chim Acta. 2023 Sep 22:1275:341567. doi: 10.1016/j.aca.2023.341567.
Xiaoyan Jia 1,  Jie Liu 1,  Yanan Zhang 1,  Xuyan Jiang 1,  Junling Zhang 2,  Jikui Wu 3
Affiliations

Affiliations

  • 1 Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai Ocean University, Shanghai, 201306, China.
  • 2 Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai, 201306, China. Electronic address: [email protected].
  • 3 Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai Ocean University, Shanghai, 201306, China; Laboratory of Quality and Safety Risk Assessment for Aquatic Product on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China. Electronic address: [email protected].
PMID: 37524461 DOI: 10.1016/j.aca.2023.341567
Abstract

Whole-cell bacteria imprinted polymer-based sensors still face challenges in the form of the difficulty of removing the template entirely, low affinity, and poor sensitivity. To further improve their performance, it is pivotal to modulate the morphology and chemical properties of imprintied polymer by taking advantage of doping engineering. Here we introduced D-tartaric acid (D-TA) as a dopant and employed pyrrole as a functional monomer to construct D-TA/polypyrrole (PPy)-based bacteria imprinted polymer (DPBIP) sensor for Vibrio parahaemolyticus (VP) detection. It is demonstrated that D-TA doping can synergistically accelerate the removal of template bacteria from imprinted Polymers (1.5 h), improve bacteria affinity of imprinted sites (the recognition time of 30 min), and enhance the sensitivity of DPBIP sensor (a detection limit of 19 CFU mL-1). The DPBIP sensor had a linear range of 102∼106 CFU mL-1 and exhibited high selectivity and good repeatability. Moreover, a recovery of 94.8%-105.3% was achieved in drinking water and oyster samples. Therefore, small Functional Molecules doping opens a new avenue to engineering BIP-based sensors with high performance, holding potential applications in securing food safety.

Keywords

Bacteria imprinted polymer; D-tartaric acid; Electro-copolymerization; Polypyrrole; Vibrio parahaemolyticus.

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