2. Academic Validation
  3. Annexin A1 exerts analgesic effect in a mouse model of medication overuse headache

Annexin A1 exerts analgesic effect in a mouse model of medication overuse headache

  • iScience. 2023 Oct 6;26(11):108153. doi: 10.1016/j.isci.2023.108153.
Zihua Gong 1 2 3,  Chunxiao Yang 1 4,  Wei Dai 1,  Shuai Miao 1,  Yingyuan Liu 1 2,  Zhiyang Jiao 5,  Bozhi Li 1,  Wei Xie 1 2,  Wei Zhao 1 2,  Xun Han 1,  Shengyuan Yu 1 2,  Zhao Dong 1 2
Affiliations

Affiliations

  • 1 Department of Neurology, The First Medical Center, Chinese PLA General Hospital, Beijing 100853, China.
  • 2 Medical School of Chinese PLA, Beijing 100853, China.
  • 3 Department of Medical Oncology, Bethune International peace Hospital, Shijiazhuang, Hebei 050082, China.
  • 4 School of Medicine, Nankai University, Tianjin 300071, China.
  • 5 Department of Outpatient, Shijiazhuang Fourth Retired Cadre Sanatorium of Hebei province Military Region, Shijiazhuang, Hebei 050082, China.
PMID: 37867938 DOI: 10.1016/j.isci.2023.108153
Abstract

Medication overuse headache (MOH) is a serious global condition. The interaction between headache attacks and medication overuse complicates the understanding of its pathophysiology. In this study, we developed a preclinical MOH model that incorporates these two key factors by overusing rizatriptan benzoate (RIZ, 4 mg/kg, i.g.) in a glyceryl trinitrate (GTN, 10 mg/kg, i.p.) induced chronic migraine mouse model. We observed that RIZ overuse aggravated GTN-induced cutaneous allodynia and caused a prolonged state of latent sensitization. We also detected a significant upregulation of Annexin-A1 (ANXA1), a protein mainly expressed in the microglia of the spinal trigeminal nucleus caudalis (SPVC), in GTN+RIZ mice. Intracerebroventricular injection of ANXA1-derived peptide Ac2-26 trifluoroacetic acid (TFA) (5 μg/mouse) inhibited bright light stress (BLS) induced acute allodynia via the formyl peptide receptor (FPR) in GTN+RIZ mice. These results suggest that ANXA1 may have an analgesic effect in triptan-associated MOH and could potentially serve as a therapeutic target.

Keywords

Biological sciences; Natural sciences; Pharmacologic activity in systems toxicology; Pharmacology; Physiology; Toxicology.

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