Combined effects of whole-body vibration and dynamic squats on cardiovascular and salivary biomarker responses in healthy adults

This study aimed to investigate the acute effects of combining whole-body vibration with dynamic squats on cardiovascular and salivary bio-marker responses in healthy adults. A randomized crossover design was conducted with 20 healthy adults. Each participant underwent three exercise sessions, with a 2-week washout period between each session. The sessions consisted of: (1) whole-body vibration (VB) at 25 Hz for 2 minutes, with an amplitude of 2 mm, and 2 minutes of rest between sets, for a total of 5 sets; (2) dynamic squats (SQ) performed 15 times within 2 minutes, with a 2-minute rest between sets, for a total of 5 sets; and (3) a combination of whole-body VB and SQ (VB+SQ). The cardiovascular variables and salivary biomarkers related to exercise intensity were assessed. Only the VB+SQ session significantly decreased the unstimulated salivary flow rate, and caused greater changes in heart rate, systolic blood pressure, mean arterial pressure, rate-pressure product, and heart rate variability compared to VB or SQ alone. Moreover, the VB+SQ session significantly increased the salivary total protein concentration from 0.56±0.05 mg/mL (baseline) to 0.74± 0.06 mg/mL (postexercise condition) and the salivary alpha-amylase activity from 33.83±5.56 U/mL (baseline) to 63.63±12.33 U/mL (postexercise condition) (P<0.05). These changes were recovered at 1-hr postexercise condition. Our findings provide information for designing exercise programs that incorporate VB+SQ to enhance hemodynamic and cardiac autonomic responses in healthy adults and for application during rehabilitation periods.

Cardiovascular system; Dynamic squats; Healthy adult; Heart rate variability; Salivary biomarker; Whole-body vibration.