2. Academic Validation
  3. ADAR1 regulates macrophage polarization and is protective against liver ischemia and reperfusion injury

ADAR1 regulates macrophage polarization and is protective against liver ischemia and reperfusion injury

  • Immunobiology. 2024 Jan;229(1):152777. doi: 10.1016/j.imbio.2023.152777.
Linxiao Wang 1,  Chujun Duan 2,  Xiuhua Wu 3,  Jiangang Xie 2,  Xiaojun Zhao 2,  Yi Si 2,  Dan Wu 2,  Yifan Wang 2,  Peng Zhao 2,  Jijun Chen 2,  Wen Yin 4,  Junjie Li 5
Affiliations

Affiliations

  • 1 Department of Emergency, Xijing Hospital, Air Force Medical University, Xi'an, China; College of Life Sciences, Northwest University, Xi'an, China.
  • 2 Department of Emergency, Xijing Hospital, Air Force Medical University, Xi'an, China.
  • 3 Department of Respiratory and Clinical Care Medicine, Shanghai Sixth People's Hospital, Shanghai, China.
  • 4 Department of Emergency, Xijing Hospital, Air Force Medical University, Xi'an, China. Electronic address: [email protected].
  • 5 Department of Emergency, Xijing Hospital, Air Force Medical University, Xi'an, China. Electronic address: [email protected].
PMID: 38113710 DOI: 10.1016/j.imbio.2023.152777
Abstract

Liver ischemia and reperfusion injury (LIRI) is a major risk for the poor prognosis of patients receiving liver transplantation. The molecular mechanism involved in LIRI is complex and related to various cellular components. We previously reported that Adenosine Deaminase acting on RNA 1 (ADAR1) alleviated the allogeneic skin graft rejection by regulating macrophage polarization. However, the regulatory effects of ADAR1 on liver macrophages after LIRI remain largely unknown. In this study, we mainly adopted a mouse model of LIRI and cellular experiments with hypoxia and reoxygenation (HR) treatment to explore the regulatory roles of ADAR1 on liver macrophages under LIRI conditions. We found that IRI caused decreased ADAR1 in liver tissues and remarkable changes of liver macrophage polarization and profiles. ADAR1 supplementation alleviated the pathological injury caused by IRI and accelerated the activation of M2 macrophages in the liver of IRI mice. Increased hypoxia duration reduced ADAR1 expression levels in murine RAW264.7 macrophages at the transcriptional level. Further overexpression of ADAR1 significantly increased the expressions of anti-inflammatory cytokines and promoted M2 polarization of macrophages under HR exposure. ADAR1 knockdown exhibited opposite effects on macrophage polarization. Hence, ADAR1 promotes the M2 polarization of liver macrophages that may further alleviate LIRI. The protective effects of ADAR1 against LIRI provide a novel insight into the prevention and treatment of LIRI.

Keywords

ADAR1; Hypoxia and reoxygenation; Ischemia and reperfusion injury; Liver; Macrophage.

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