2. Academic Validation
  3. Plasmodesmata-associated Flotillin positively regulates broad-spectrum virus cell-to-cell trafficking

Plasmodesmata-associated Flotillin positively regulates broad-spectrum virus cell-to-cell trafficking

  • Plant Biotechnol J. 2023 Dec 21. doi: 10.1111/pbi.14274.
Panpan Ge # 1 2,  Hong Lu # 1,  Wei Wang 1,  Yonghuan Ma 1 2,  Yi Li 3,  Tong Zhou 4,  Taiyun Wei 5,  Jianguo Wu 5,  Feng Cui 1 2
Affiliations

Affiliations

  • 1 State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
  • 2 University of Chinese Academy of Sciences, Beijing, China.
  • 3 State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, China.
  • 4 Key Laboratory of Food Quality and Safety, Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
  • 5 State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Vector-borne Virus Research Center, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, China.
  • # Contributed equally.
PMID: 38130080 DOI: 10.1111/pbi.14274
Abstract

Viral diseases seriously threaten rice production. Plasmodesmata (PD)-associated proteins are deemed to play a key role in viral Infection in host Plants. However, few PD-associated proteins have been discovered in rice to afford viral Infection. Here, inspired by the Infection mechanism in insect vectors, we identified a member of the Flotillin family taking part in the cell-to-cell transport of rice stripe virus (RSV) in rice. Flotillin1 interacted with RSV nucleocapsid protein (NP) and was localized on PD. In flotillin1 knockout mutant rice, which displayed normal growth, RSV intercellular movement was retarded, leading to significantly decreased disease incidence. The PD pore sizes of the mutant rice were smaller than those of the wild type due to more callose deposits, which was closely related to the upregulation of two callose synthase genes. RSV Infection stimulated flotillin1 expression and enlarged the PD aperture via RSV NP. In addition, flotillin1 knockout decreased disease incidences of southern rice black-streaked dwarf virus (SRBSDV) and rice dwarf virus (RDV) in rice. Overall, our study reveals a new PD-associated protein facilitating virus cell-to-cell trafficking and presents the potential of flotillin1 as a target to produce broad-spectrum Antiviral rice resources in the future.

Keywords

Flotillin; plasmodesmata-associated protein; rice; rice dwarf virus; rice stripe virus; southern rice black-streaked dwarf virus.

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