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  2. FM4-64

FM4-64 

Cat. No.: HY-103466 Purity: 99.94%
Handling Instructions

FM4-64, a membrane-selective red fluorescent dye, belongs to the FM family of styrylpyridinium dyes. FM4-64 is widely used to study endocytosis and exocytosis, vesicle trafficking and organelle organization in living animal.

For research use only. We do not sell to patients.

FM4-64 Chemical Structure

FM4-64 Chemical Structure

CAS No. : 162112-35-8

Size Price Stock Quantity
1 mg USD 410 In-stock
Estimated Time of Arrival: December 31
5 mg USD 1230 In-stock
Estimated Time of Arrival: December 31
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50 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

FM4-64, a membrane-selective red fluorescent dye, belongs to the FM family of styrylpyridinium dyes. FM4-64 is widely used to study endocytosis and exocytosis, vesicle trafficking and organelle organization in living animal[1][2].

In Vitro

FM4-64 readily translocates to the nuclear envelope (NE) at physiological temperature where it exhibits enhanced fluorescence when excited at 620-650 nm in contrast to 480-520 nm excitation in the endocytic pathway and in the endoplasmic reticulum (ER). In vitro data indicated that the dye reveals an enrichment of negatively charged lipids, presumably due to local phospholipid synthesis. Dual-excitation imaging of FM4-64 in relation to lamina-associated polypeptide-1-green fluorescent protein during mitosis suggested that the disassembly of NE preserves microscale lipid complexes in the ER[1].
For studies onplant and fungal cells, FM4-64 is usually preferred to FM1-43 due to its superior brightness, greater contrast, higher photostability, and its red-shifted emission facilitating concomitantuse with the green fluorescent protein (GFP).[3]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

607.51

Formula

C30H45Br2N3

CAS No.
SMILES

CC[N+](CCC[N+]1=CC=C(/C=C/C=C/C=C/C2=CC=C(N(CC)CC)C=C2)C=C1)(CC)CC.[Br-].[Br-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (82.30 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6461 mL 8.2303 mL 16.4606 mL
5 mM 0.3292 mL 1.6461 mL 3.2921 mL
10 mM 0.1646 mL 0.8230 mL 1.6461 mL
*Please refer to the solubility information to select the appropriate solvent.
Purity & Documentation

Purity: 99.94%

References
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Product Name:
FM4-64
Cat. No.:
HY-103466
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