2. Academic Validation
  3. MDM4 was associated with poor prognosis and tumor-immune infiltration of cancers

MDM4 was associated with poor prognosis and tumor-immune infiltration of cancers

  • Eur J Med Res. 2024 Jan 27;29(1):79. doi: 10.1186/s40001-024-01684-z.
Jie Liu # 1 2 3,  Jie Yang # 2 3,  Qilong Pan 1,  Xiangyu Wang 1 2 3,  Xinyin Wang 1,  Han Chen 1,  Xiaoling Zheng 4 5,  Qingling Huang 6
Affiliations

Affiliations

  • 1 Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China.
  • 2 Department of Endoscopy, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou, China.
  • 3 The Graduate School of Fujian Medical University, Fuzhou, China.
  • 4 Department of Endoscopy, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou, China. [email protected].
  • 5 The Graduate School of Fujian Medical University, Fuzhou, China. [email protected].
  • 6 Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China. [email protected].
  • # Contributed equally.
PMID: 38281029 DOI: 10.1186/s40001-024-01684-z
Abstract

MDM4 is one of the MDM protein family and is generally recognized as the key negative regulator of p53. As a cancer-promoting factor, it plays a non-negligible role in tumorigenesis and development. In this article, we analyzed the expression levels of MDM4 in pan-cancer through multiple databases. We also investigated the correlations between MDM4 expression and prognostic value, immune features, genetic mutation, and tumor-related pathways. We found that MDM4 overexpression is often accompanied by adverse clinical features, poor prognosis, oncogenic mutations, tumor-immune infiltration and aberrant activation of oncogenic signaling pathways. We also conducted transcriptomic sequencing to investigate the effect of MDM4 on transcript levels in colon Cancer and performed qPCR to verify this. Finally, we carried out some in vitro experiments including colony formation assay, chemoresistance and senescence-associated β-galactosidase activity assay to study the anti-tumor treatment effect of small molecule MDM4 inhibitor, NSC146109. Our research confirmed that MDM4 is a prognostic biomarker and potential therapeutic target for a variety of malignancies.

Keywords

Inhibitor; MDM4; Pan-cancer; Prognostic biomarker; Tumor-immune infiltration.

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