2. Academic Validation
  3. CCCP inhibits DPV infection in DEF cells by attenuating DPV manipulated ROS, apoptosis, and mitochondrial stability

CCCP inhibits DPV infection in DEF cells by attenuating DPV manipulated ROS, apoptosis, and mitochondrial stability

  • Poult Sci. 2024 Apr;103(4):103446. doi: 10.1016/j.psj.2024.103446.
Shuyi He 1,  Bin Tian 1,  Huanhuan Cao 1,  Mingshu Wang 1,  Dongjie Cai 2,  Ying Wu 1,  Qiao Yang 1,  Xumin Ou 1,  Di Sun 1,  Shaqiu Zhang 1,  Sai Mao 1,  XinXin Zhao 1,  Juan Huang 1,  Dekang Zhu 1,  Renyong Jia 1,  Shun Chen 1,  Mafeng Liu 1,  Anchun Cheng 3
Affiliations

Affiliations

  • 1 Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu City, 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China.
  • 2 Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu City, 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China.
  • 3 Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu City, 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China. Electronic address: [email protected].
PMID: 38377689 DOI: 10.1016/j.psj.2024.103446
Abstract

Duck plague virus (DPV) is extremely infectious and lethal, so Antiviral drugs are urgently needed. Our previous study shows that DPV Infection with duck embryo fibroblast (DEF) induces Reactive Oxygen Species (ROS) changes and promotes Apoptosis. In this study, we tested the Antiviral effect of the carbonyl cyanide m-chlorophenyl hydrazone (CCCP), a common mitochondrial Autophagy Inducer. Our results demonstrated a dose-dependent anti-DPV effect of CCCP, CCCP-treatment blocked the intercellular transmission of DPV after Infection, and we also proved that CCCP could have an Antiviral effect up to 48 hpi. The addition of CCCP reversed the DPV-induced ROS changes, CCCP can inhibit virus-induced apoptosis; meanwhile, CCCP can affect mitochondrial fusion and activate Mitophagy to inhibit DPV. In conclusion, CCCP can be an effective Antiviral candidate against DPV.

Keywords

CCCP; antiviral; apoptosis; duck plague virus; mitochondria.

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