2. Academic Validation
  3. Hypoxic environment promotes angiogenesis and bone bridge formation by activating Notch/RBPJ signaling pathway in HUVECs

Hypoxic environment promotes angiogenesis and bone bridge formation by activating Notch/RBPJ signaling pathway in HUVECs

  • Genomics. 2024 May;116(3):110838. doi: 10.1016/j.ygeno.2024.110838.
Wendong Liu 1,  Mincheng Zou 1,  Mimi Chen 2,  Zheng Zhang 2,  Yunpeng Mao 2,  Yuhao Yang 2,  Ya Liu 2,  Qin Shi 3,  Xiaodong Wang 1,  Fuyong Zhang 4
Affiliations

Affiliations

  • 1 Department of Orthopaedics, Children's Hospital of Soochow University, 92 Zhongnan St., Suzhou 215000, Jiangsu Province, China; Clinical Pediatrics School, Soochow University, 92 Zhongnan St., Suzhou 215000, Jiangsu Province, China.
  • 2 Department of Orthopaedics, Children's Hospital of Soochow University, 92 Zhongnan St., Suzhou 215000, Jiangsu Province, China.
  • 3 Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, 188 Shizi St., Suzhou 215006, Jiangsu, China.
  • 4 Department of Orthopaedics, Children's Hospital of Soochow University, 92 Zhongnan St., Suzhou 215000, Jiangsu Province, China. Electronic address: [email protected].
PMID: 38537807 DOI: 10.1016/j.ygeno.2024.110838
Abstract

After epiphyseal fracture, the epiphyseal plate is prone to ischemia and hypoxia, leading to the formation of bone bridge and deformity. However, the exact mechanism controlling the bone bridge formation remains unclear. Notch/RBPJ signaling axis has been indicated to regulate angiogenesis and osteogenic differentiation. Our study aims to investigate the mechanism of bone bridge formation after epiphyseal plate injury, and to provide a theoretical basis for new therapeutic approaches to prevent the bone bridge formation. The expression of DLL4 and RBPJ was significantly up-regulated in HUVECs after ischemia and hypoxia treatment. Notch/RBPJ pathway positively regulated the osteogenic differentiation of BMSCs. HUVECs can induce osteogenic differentiation of BMSCs under ischemia and hypoxia. Notch/RBPJ pathway is involved in the regulation of the trans-epiphyseal bridge formation. Notch/RBPJ in HUVECs is associated with osteogenic differentiation of BMSCs and may participate in the regulation of the bone bridge formation across the epiphyseal plate.

Keywords

Bone bridge; Bone mesenchymal stem cells; Epiphyseal plate injury; Human umbilical vein endothelial cells; Notch/RBPJ signaling pathway; Osteogenic differentiation.

Figures
Products