1. Neuronal Signaling
    Stem Cell/Wnt
  2. Notch
  3. RBPJ Inhibitor-1

RBPJ Inhibitor-1 (Synonyms: RIN1)

Cat. No.: HY-137471 Purity: 99.11%
Handling Instructions

RBPJ Inhibitor-1 (RIN1), the first RBPJ inhibitor, blocks the functional interaction of RBPJ with SHARP. RBPJ Inhibitor-1 (RIN1) inhibits NOTCH-dependent tumor cell proliferation.

For research use only. We do not sell to patients.

RBPJ Inhibitor-1 Chemical Structure

RBPJ Inhibitor-1 Chemical Structure

Size Price Stock Quantity
5 mg USD 100 In-stock
Estimated Time of Arrival: December 31
10 mg USD 170 In-stock
Estimated Time of Arrival: December 31
25 mg USD 350 In-stock
Estimated Time of Arrival: December 31
50 mg USD 600 In-stock
Estimated Time of Arrival: December 31
100 mg USD 980 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

RBPJ Inhibitor-1 (RIN1), the first RBPJ inhibitor, blocks the functional interaction of RBPJ with SHARP. RBPJ Inhibitor-1 (RIN1) inhibits NOTCH-dependent tumor cell proliferation[1].

In Vitro

RIN1 inhibits the proliferation of hematologic cancer cell lines and promoted skeletal muscle diferentiation from C2C12 myoblasts[1].
RIN1 (0.6 µM, corresponding to 3 × IC50) decreases the number of MHC+ cells and increased the number of nuclei per cell, indicating that it induces the formation of multinucleated myofbers[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: AD-293 cells were transfected with RBPJ-VP16myc.
Concentration: 2 μM.
Incubation Time: 17 hours.
Result: Inhibited Hes1-Luciferase activity with an IC50 of 0.18 µM and Emax of 82%.
Inhibited NOTCH3 ICD with similar potency and efcacy (0.19 µM and Emax=88%).

Cell Proliferation Assay[1]

Cell Line: T-cell acute lymphoblastic leukemia (T-ALL) patients (Jurkat and KOPT-K1) and in the mantle cell lymphoma (MCL) line REC-1.
Concentration: 0.1-10 μM.
Incubation Time: 96 h.
Result: Inhibited NOTCH-dependent tumor cell proliferation.
Was active in both the cancer cell anti-proliferation and myoblast diferentiation assays.
Molecular Weight

311.31

Formula

C₁₇H₁₄FN₃O₂

SMILES

O=C(N)C1=CC=C(C2=CC=NN2C)C=C1OC3=CC=CC=C3F

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (803.06 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2122 mL 16.0612 mL 32.1223 mL
5 mM 0.6424 mL 3.2122 mL 6.4245 mL
10 mM 0.3212 mL 1.6061 mL 3.2122 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (6.68 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (6.68 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (6.68 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

RBPJ Inhibitor-1RIN1RBPJ Inhibitor1RBPJ Inhibitor 1RIN 1RIN-1NotchT-ALLnotchMCLREC-1T-cellacutelymphoblasticleukemiamantlecelllymphomaInhibitorinhibitorinhibit

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Product Name:
RBPJ Inhibitor-1
Cat. No.:
HY-137471
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