2. Academic Validation
  3. Generation of a TSC2 knockout embryonic stem cell line by CRISPR/Cas9 editing

Generation of a TSC2 knockout embryonic stem cell line by CRISPR/Cas9 editing

  • Stem Cell Res. 2024 Jun:77:103399. doi: 10.1016/j.scr.2024.103399.
Siyao Zhang 1,  Jiaqi Fan 1,  Hairui Sun 1,  Xiaoyan Hao 2,  Yihua He 3
Affiliations

Affiliations

  • 1 Anzhen Hospital, Capital Medical University, Beijing 100029, China; Maternal-Fetal Medicine Centre in Fetal Heart Disease, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
  • 2 Anzhen Hospital, Capital Medical University, Beijing 100029, China; Maternal-Fetal Medicine Centre in Fetal Heart Disease, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China. Electronic address: [email protected].
  • 3 Anzhen Hospital, Capital Medical University, Beijing 100029, China; Maternal-Fetal Medicine Centre in Fetal Heart Disease, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China. Electronic address: [email protected].
PMID: 38574666 DOI: 10.1016/j.scr.2024.103399
Abstract

Tuberous Sclerosis Complex (TSC) is a severe developmental disorder with various clinical effects, primarily caused by TSC2 gene mutations, often involving loss of function(Henske,et al., 2016).To explore role of TSC2 in human heart development, we successfully developed a TSC2 knockout (TSC2-/-) human embryonic stem cells (hESCs) line using CRISPR/Cas9 gene editing. This TSC2-/- hESC line maintained a normal karyotype, expressed pluripotency markers strongly, and could differentiate into all three germ layers in vivo. This cell line will be a valuable tool for future research on the role of TSC2 in heart development.

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