Heme oxygenase activates calcium release from the endoplasmic reticulum of bovine mammary epithelial cells to promote TFEB entry into the nucleus to reduce the intracellular load of Mycoplasma bovis

Heme oxygenase HO-1 (HMOX) regulates cellular inflammation and Apoptosis, but its role in regulation of Autophagy in Mycoplasma bovis Infection is unknown. The objective was to determine how the HO-1/CO- Protein kinase RNA-like endoplasmic reticulum kinase (PERK)-Ca²⁺- transcription factor EB (TFEB) signaling axis induces Autophagy and regulates clearance of M. bovis by bovine mammary epithelial cells (bMECs). M. bovis inhibited Autophagy and lysosomal biogenesis in bMECs and suppressed HO-1 protein and expression of related proteins, namely nuclear factor erythroid 2-related factor 2 (Nrf2) and Kelch-like ECH-associated protein 1 (keap1). Activation of HO-1 and its production of carbon monoxide (CO) were required for induction of Autophagy and clearance of intracellular M. bovis. Furthermore, when HO-1 was deficient, CO sustained cellular Autophagy. HO-1 activation increased intracellular calcium (Ca²⁺) and cytosolic localization activity of TFEB via PERK. Knockdown of PERK or chelation of intracellular Ca²⁺ inhibited HO-1-induced M. bovis Autophagy and clearance. M. bovis Infection affected nuclear localization of lysosomal TFEB in the MiT/TFE transcription factor subfamily, whereas activation of HO-1 mediated dephosphorylation and intranuclear localization of TFEB, promoting Autophagy, lysosomal biogenesis and autophagic clearance of M. bovis. Nuclear translocation of TFEB in HO-1 was critical to induce M. bovis transport and survival of infected bMECs. Furthermore, the HO-1/CO-PERK-Ca²⁺-TFEB signaling axis induced Autophagy and M. bovis clearance, providing a viable approach to treat persistent M. bovis infections.

Autophagy; Ca(2+); HO-1; Mycoplasma bovis; TFEB; bMECs.