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  3. Hemin

Hemin  (Synonyms: Hemin chloride)

Cat. No.: HY-19424 Purity: ≥98.0%
COA Handling Instructions

Hemin is an iron-containing porphyrin. Hemin is an Heme oxygenase (HO)-1 inducer.

For research use only. We do not sell to patients.

Hemin Chemical Structure

Hemin Chemical Structure

CAS No. : 16009-13-5

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Customer Review

Based on 21 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Hemin purchased from MCE. Usage Cited in: Genome Biol. 2022 Dec 15;23(1):259.  [Abstract]

    Hemin (0, 20, 40, 80 µM; 2 h) increases G4 levels in a dose-dependent manner in HeLa cells. Immunostaining of G4 using BG4-EGFP (green). The nuclei are stained with DAPI (blue).
    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Hemin is an iron-containing porphyrin. Hemin is an Heme oxygenase (HO)-1 inducer.

    IC50 & Target

    Heme oxygenase[1]

    In Vitro

    Hemin and PGJ2, used as positive controls, strongly increase both expression and activity of HMOX after 4 and 12 h, respectively. Indeed, a significant effect is found of 30 μM Hemin on cell proliferation in all used cell lines after 48 h, which is dose-dependent. Hemin treatment decreases cell proliferation to 62±5 %, 51±3 %, and 38±8 % in PA-TU-8902, BxPC-3 and MiaPaCa-2 cancer cells, respectively, with p<0.0001 for all comparisons. Furthermore, enhancement of anti-proliferative effects of statins is observed by Hemin, documented as decreased cell proliferation after 48 h of co-treatment[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    Following the i.p. administration of Hemin (100 μmol/kg), the HO-1 level in the renal cortex begins to increase gradually. The HO-1 level reaches its peak 24 h after Hemin preconditioning. HO-1 is expressed mainly in the renal tubules. The HO-2 level in the kidney does not change following Hemin preconditioning[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Clinical Trial
    Molecular Weight

    651.94

    Appearance

    Solid

    Formula

    C34H32ClFeN4O4

    CAS No.
    SMILES

    [Cl-][Fe+3]123[N-]4C5=C(CCC([O-])=O)C(C)=C4C=C(C(C=C)=C6C)[N]1=C6C=C(C(C=C)=C7C)[N-]2C7=CC(C(C)=C8CCC([O-])=O)=[N]3C8=C5.[H+].[H+]

    Structure Classification
    Source

    Red blood cells

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, protect from light, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

    Solvent & Solubility
    In Vitro: 

    1M NaOH : 6.67 mg/mL (10.23 mM; ultrasonic and adjust pH to 12 with NaOH)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.5339 mL 7.6694 mL 15.3388 mL
    5 mM 0.3068 mL 1.5339 mL 3.0678 mL
    10 mM 0.1534 mL 0.7669 mL 1.5339 mL
    *Please refer to the solubility information to select the appropriate solvent.
    Purity & Documentation

    Purity: ≥98.0%

    References
    Cell Assay
    [1]

    For the cell proliferation assay, cells are seeded into 96 well (5-12.5×104 cells per mL according to the cell line) and kept at 37°C and 5 % CO2. After 24 h, cells are treated with statins or/and Hemin, followed by the MTT test as a general cell proliferation assay[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [2]

    Mice[2]
    Eight- to ten-week-old male BABL/c mice are used for the ischemia-reperfusion (I/R) experiments. The animals are divided into five groups as follows: (1) the sham group undergo isolation of the bilateral renal arteries without clamping; (2) the vehicle group receive an intraperitoneal (i.p.) injection of 4 mL/kg PBS as a vehicle control (with IRI); (3) the Hemin-preconditioned group receive Hemin, a potent inducer of HO-1, at 100 μmol/kg i.p.; (4) the Hemin plus ZnPP group receive zinc protoporphyrin IX, an inhibitor of HO-1 activity, at 5 mg/kg i.p. 6 h after receiving 100 μmol/kg Hemin i.p.; and (5) the Hemin plus PD98059 group receive PD98059, an inhibitor of ERK1/2 activity, at 10 mg/kg i.p. 6 h after receiving 100 μmol/kg Hemin i.p. Both inhibitors are administered i.p. 2 h before I/R, whereas Hemin was administered 8 h before I/R.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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