Hemin
Based on 87 publication(s) in Google Scholar
Hemin is an iron-containing porphyrin. Hemin is an Heme oxygenase (HO)-1 inducer.
For research use only. We do not sell to patients.
- Purity: 99.59%
- CAS No.: 16009-13-5
- Formula: C34H32ClFeN4O4
- Molecular Weight:651.94
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Hemin
More- Cell Metab. 2025 Jan 7;37(1):87-103.e10. [Abstract]
- Cell Mol Immunol. 2022 Apr;19(4):516-526. [Abstract]
- Mol Cell. 2025 Dec 4;85(23):4463-4479.e7. [Abstract]
- ACS Nano. 2025 Aug 19;19(32):29341-29359. [Abstract]
- Adv Sci (Weinh). 2025 Nov 6:e07384. [Abstract]
- Adv Sci (Weinh). 2025 Jan 30:e2411923. [Abstract]
- Adv Sci (Weinh). 2023 Aug;10(22):e2206798. [Abstract]
- Exp Mol Med. 2025 May;57(5):1064-1077. [Abstract]
- Redox Biol. 2025 May:82:103550. [Abstract]
- J Control Release. 2025 May 6:113801. [Abstract]
- Cell Rep Med. 2025 Oct 21;6(10):102410. [Abstract]
- Cell Rep Med. 2025 Sep 25:102371. [Abstract]
- J Immunother Cancer. 2024 Nov 24;12(11):e009805. [Abstract]
- Cancer Lett. 2022 Oct 10:546:215831. [Abstract]
- Cell Death Dis. 2025 Jan 18;16(1):28. [Abstract]
- Genome Biol. 2025 Feb 28;26(1):44. [Abstract]
- Genome Biol. 2022 Dec 15;23(1):259. [Abstract]
- Int J Biol Macromol. 2025 Sep 2;327(Pt 2):147257. [Abstract]
- Int J Biol Macromol. 2025 Jan 31:140542. [Abstract]
- Free Radic Biol Med. 2022 Nov 20;193(Pt 2):795-807. [Abstract]
- Sens Actuators B Chem. 2025 Jul 1.
- Biomed Pharmacother. 2025 Mar 25:186:118008. [Abstract]
- Redox Rep. 2025 Dec;30(1):2529695. [Abstract]
- PLoS Biol. 2025 Nov 24;23(11):e3003490. [Abstract]
- Cell Chem Biol. 2025 Jun 19;32(6):854-871.e6. [Abstract]
- Microbiol Res. 2024 Jul:284:127727. [Abstract]
- Anal Chem. 2025 Jul 1;97(25):13542-13550. [Abstract]
- Anal Chem. 2025 Feb 18;97(6):3680-3686. [Abstract]
- Neural Regen Res. 2024 Jul 1;19(7):1568-1574. [Abstract]
- Neural Regen Res. 2020 Jul;15(7):1274-1282. [Abstract]
- Antioxidants (Basel). 2026 Jan 21;15(1):135. [Abstract]
- Antioxidants (Basel). 2021 Jun 29;10(7):1049. [Abstract]
- Int J Nanomedicine. 2021 Feb 26:16:1565-1573. [Abstract]
- Fluids Barriers CNS. 2025 Apr 14;22(1):37. [Abstract]
- Ecotoxicol Environ Saf. 2025 Jun 6:300:118486. [Abstract]
- Biomater Adv. 2026 Mar:180:214595. [Abstract]
- J Invest Dermatol. 2022 Jan;142(1):189-200.e8. [Abstract]
- Cell Prolif. 2024 Jul 24:e13720. [Abstract]
- Am J Chin Med. 2026;54(2):603-625. [Abstract]
- Mar Drugs. 2025 Mar 28;23(4):146. [Abstract]
- Inflamm Res. 2024 Oct;73(10):1727-1745. [Abstract]
- J Mol Liq. 2026 May 23;457:129686.
- CNS Neurosci Ther. 2025 Sep;31(9):e70609. [Abstract]
- PLoS Pathog. 2026 Mar 16;22(3):e1014042. [Abstract]
- Int J Mol Sci. 2023 Nov 2;24(21):15920. [Abstract]
- Biomolecules. 2023 Jul 10;13(7):1097. [Abstract]
- Front Pharmacol. 2021 Mar 19:12:629379. [Abstract]
- Eur J Pharmacol. 2025 Oct 11:1007:178232. [Abstract]
- Int Immunopharmacol. 2025 Sep 15:166:115548. [Abstract]
- Int Immunopharmacol. 2025 May 12:158:114827. [Abstract]
- Eur J Pharmacol. 2024 Jul 5:974:176620. [Abstract]
- Eur J Pharm Sci. 2023 Oct 1:189:106550. [Abstract]
- Eur J Pharmacol. 2022 Feb 15:917:174722. [Abstract]
- Int Immunopharmacol. 2020 Mar;80:106141. [Abstract]
- Mol Pharm. 2025 Aug 4;22(8):4969-4982. [Abstract]
- ACS Omega. 2024 Dec 19;10(1):1188-1197. [Abstract]
- FASEB J. 2025 Oct 15;39(19):e71115. [Abstract]
- Drug Dev Res. 2025 May;86(3):e70105. [Abstract]
- Drug Dev Res. 2024 Sep;85(6):e22245. [Abstract]
- J Inflamm Res. 2025 Jun 7:18:7401-7417. [Abstract]
- Neurochem Int. 2026 Mar 28:196:106150. [Abstract]
- J Neurochem. 2021 Sep;158(5):1110-1130. [Abstract]
- Fish Shellfish Immunol. 2026 May:172:111209. [Abstract]
- Sci Rep. 2025 May 13;15(1):16595. [Abstract]
- ACS Chem Neurosci. 2023 Mar 15;14(6):1071-1079. [Abstract]
- Neurochem Res. 2025 Sep 6;50(5):291. [Abstract]
- Reproduction. 2021 Dec 27;163(1):33-43. [Abstract]
- Exp Cell Res. 2022 Jan 1;410(1):112946. [Abstract]
- PLoS Negl Trop Dis. 2025 Dec 11;19(12):e0013815. [Abstract]
- Appl Biochem Biotechnol. 2026 Mar 23. [Abstract]
- Cytotherapy. 2023 Jul;25(7):728-738. [Abstract]
- Arch Biochem Biophys. 2026 Apr:778:110746. [Abstract]
- J Biochem Mol Toxicol. 2026 Jan;40(1):e70677. [Abstract]
- J Biochem Mol Toxicol. 2026 Jan;40(1):e70661. [Abstract]
- Front Neurol. 2018 Nov 6:9:931. [Abstract]
- J Chem. 2025 Dec 19;2025(1):7819395.
- Brain Res. 2025 Nov 1:1866:149927. [Abstract]
- Clin Exp Pharmacol Physiol. 2022 Aug;49(8):858-870. [Abstract]
- Am J Reprod Immunol. 2024 Aug;92(2):e13916. [Abstract]
- Stem Cells Dev. 2023 Jun;32(11-12):346-363. [Abstract]
- J Vis Exp. 2025 Oct 31:(224). [Abstract]
- Northwestern University. 2026.
- Res Sq. 2025 Jun 19.
- bioRxiv. 2024 October 27.
- SSRN. 2023 Oct 10.
- Research Square Print. August 19th, 2022.
- Research Square Print. 2022 Jul.
Customer Validation & Images
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In Vivo Efficacy Study
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IHC
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Biological Activity
Heme oxygenase[1]
Hemin and PGJ2, used as positive controls, strongly increase both expression and activity of HMOX after 4 and 12 h, respectively. Indeed, a significant effect is found of 30 μM Hemin on cell proliferation in all used cell lines after 48 h, which is dose-dependent. Hemin treatment decreases cell proliferation to 62±5 %, 51±3 %, and 38±8 % in PA-TU-8902, BxPC-3 and MiaPaCa-2 cancer cells, respectively, with p<0.0001 for all comparisons. Furthermore, enhancement of anti-proliferative effects of statins is observed by Hemin, documented as decreased cell proliferation after 48 h of co-treatment[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 16009-13-5
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Appearance Solid
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Molecular Weight 651.94
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Formula C34H32ClFeN4O4
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Color Dark purple to black
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SMILES
[Cl-][Fe+3]123[N-]4C5=C(CCC(O)=O)C(C)=C4C=C(C(C=C)=C6C)[N]1=C6C=C(C(C=C)=C7C)[N-]2C7=CC(C(C)=C8CCC(O)=O)=[N]3C8=C5
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Synonyms
Hemin chloride
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Structure Classification
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Initial Source
Red blood cells
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (87)
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Journal Impact Factor
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Most Recent
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Cell Metab
Stress triggers irritable bowel syndrome with diarrhea through a spermidine-mediated decline in type I interferon. [Abstract]2025 Jan 7;37(1):87-103.e10. PMID: 39366386 -
Cell Mol Immunol
2022 Apr;19(4):516-526. PMID: 34983952
Hemin purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2022 Apr;19(4):516-526. [Abstract]
MCA205 and B16 cells treated with 20 μM Hemin or vehicle were infected with HSV-1 (0.25 MOI), and the expression level of BACH1 and genome copies of HSV-1 were measured by western blotting and qRT–PCR, respectively, at 24 hpi.
Hemin purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2022 Apr;19(4):516-526. [Abstract]
Hemin (50 mg/kg hemin daily by intraperitoneal injection) treatment further enhanced CD45+CD8+ T lymphocyte infiltration in MCA205 fibrosarcoma tumors infected with HSV-1.
Hemin purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2022 Apr;19(4):516-526. [Abstract]
Hemin (50 mg/kg hemin daily by intraperitoneal injection) treatment further enhanced CD45+CD8+ T lymphocyte infiltration in MCA205 fibrosarcoma tumors infected with HSV-1.
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Mol Cell
High-throughput capture of actively transcribed region-interacting sequences reveals an intricate promoter-centered regulatory network. [Abstract]2025 Dec 4;85(23):4463-4479.e7. PMID: 41260212 -
ACS Nano
Synergizing Ferroptosis Suppressor Protein 1 Gene Silencing and Photodynamic Therapy Based on Photosensitive Lipid Nanoparticles for Colon Cancer Immunotherapy. [Abstract]2025 Aug 19;19(32):29341-29359. PMID: 40772591 -
Adv Sci (Weinh)
Iron Overload Mediates the Differential Cell Fate of Astrocytes from Neurons and Its Regulatory Mechanisms in Ischemic Stroke. [Abstract]2025 Nov 6:e07384. PMID: 41195589 -
Adv Sci (Weinh)
2025 Jan 30:e2411923. PMID: 39888292 -
Adv Sci (Weinh)
Donafenib and GSK-J4 Synergistically Induce Ferroptosis in Liver Cancer by Upregulating HMOX1 Expression. [Abstract]2023 Aug;10(22):e2206798. PMID: 37330650
Hemin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Aug;10(22):e2206798. [Abstract]
Hemin (dissolved in DMSO to the required concentration and stirred at RT for 2 h.) induced ferroptosis more rapidly when treated in combination with donafenib+GSK-J4.
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Exp Mol Med
Acetylation of α-tubulin restores endothelial cell injury and blood-brain barrier disruption after intracerebral hemorrhage in mice. [Abstract]2025 May;57(5):1064-1077. PMID: 40335634 -
Redox Biol
FOXO3a-BAP1 axis regulates neuronal ferroptosis in early brain injury after subarachnoid hemorrhage. [Abstract]2025 May:82:103550. PMID: 40080966 -
J Control Release
Virus-mimicking nanoparticle potentiates in-situ cancer vaccines by reversing intratumoral DCs via stimulating cytosolic nucleic acid sensors. [Abstract]2025 May 6:113801. PMID: 40339656 -
Cell Rep Med
Intestinal Lachnospiraceae bacterium-derived propionate inhibits the progression of clear cell renal cell carcinoma. [Abstract]2025 Oct 21;6(10):102410. PMID: 41067230 -
Cell Rep Med
Vorapaxar enhanced mitochondria-associated ferroptosis primes cancer immunotherapy via targeting FOXO1/HMOX1 axis. [Abstract]2025 Sep 25:102371. PMID: 41005298 -
J Immunother Cancer
Propafenone facilitates mitochondrial-associated ferroptosis and synergizes with immunotherapy in melanoma. [Abstract]2024 Nov 24;12(11):e009805. PMID: 39581704 -
Cancer Lett
2022 Oct 10:546:215831. PMID: 35868533 -
Cell Death Dis
Inhibition of KLF5 promotes ferroptosis via the ZEB1/HMOX1 axis to enhance sensitivity to oxaliplatin in cancer cells. [Abstract]2025 Jan 18;16(1):28. PMID: 39827156 -
Genome Biol
CTCF is selectively required for maintaining chromatin accessibility and gene expression in human erythropoiesis. [Abstract]2025 Feb 28;26(1):44. PMID: 40022213 -
Genome Biol
G-quadruplexes sense natural porphyrin metabolites for regulation of gene transcription and chromatin landscapes. [Abstract]2022 Dec 15;23(1):259. PMID: 36522639
Hemin purchased from MedChemExpress. Usage Cited in: Genome Biol. 2022 Dec 15;23(1):259. [Abstract]
Hemin (0, 20, 40, 80 µM; 2 h) increases G4 levels in a dose-dependent manner in HeLa cells. Immunostaining of G4 using BG4-EGFP (green). The nuclei are stained with DAPI (blue).
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Int J Biol Macromol
Heme oxygenase-1 (HO-1) depletion promotes ferroptosis to reverse cisplatin-resistance via enhancing NCOA4-mediated ferritinophagy in ovarian cancer. [Abstract]2025 Sep 2;327(Pt 2):147257. PMID: 40907917 -
Int J Biol Macromol
Recombinant human heavy-chain ferritin nanoparticles loaded with rosuvastatin attenuates secondary brain injury in intracerebral hemorrhage. [Abstract]2025 Jan 31:140542. PMID: 39894117 -
Free Radic Biol Med
Taurine attenuates neuronal ferroptosis by regulating GABAB/AKT/GSK3β/β-catenin pathway after subarachnoid hemorrhage. [Abstract]2022 Nov 20;193(Pt 2):795-807. PMID: 36402441 -
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Biomed Pharmacother
Excess heme orchestrates progesterone resistance in uterine endometrial cancer through macrophage polarization and the IL-33/PAX8/PGR axis. [Abstract]2025 Mar 25:186:118008. PMID: 40138919 -
Redox Rep
Sesamin protects against Acetaminophen-induced nephrotoxicity by suppressing HMOX1-mediated apoptosis and ferroptosis. [Abstract]2025 Dec;30(1):2529695. PMID: 40650944 -
PLoS Biol
The cell cycle regulator PLK1 promotes murine melanoma progression by regulating the transcription factor BACH1. [Abstract]2025 Nov 24;23(11):e3003490. PMID: 41284701 -
Cell Chem Biol
Mechanism by which the molecular glue-like verteporfin induces IRE1α dimerization and activation to synergize with AKT inhibition in breast cancer. [Abstract]2025 Jun 19;32(6):854-871.e6. PMID: 40472846 -
Microbiol Res
Heme oxygenase activates calcium release from the endoplasmic reticulum of bovine mammary epithelial cells to promote TFEB entry into the nucleus to reduce the intracellular load of Mycoplasma bovis. [Abstract]2024 Jul:284:127727. PMID: 38636241 -
Anal Chem
Application of Non-G-Quadruplex Hemin Aptamers to Hemin Detection and Heme Oxygenase 1 Activity Evaluation via Spatial Conformational Constraint. [Abstract]2025 Jul 1;97(25):13542-13550. PMID: 40526855 -
Anal Chem
Exploration and Application of the Catalytic Superiority of Non-G-Quadruplex Hemin Aptamers. [Abstract]2025 Feb 18;97(6):3680-3686. PMID: 39907727 -
Neural Regen Res
Fibroblast growth factor 21 inhibits ferroptosis following spinal cord injury by regulating heme oxygenase-1. [Abstract]2024 Jul 1;19(7):1568-1574. PMID: 38051901 -
Neural Regen Res
2020 Jul;15(7):1274-1282. PMID: 31960813 -
Antioxidants (Basel)
Betaine Inhibits Ferroptosis After Intracerebral Hemorrhage by Activating the Nrf2/HO-1 Pathway. [Abstract]2026 Jan 21;15(1):135. PMID: 41596193 -
Antioxidants (Basel)
Characterization of the Inducible and Slow-Releasing Hydrogen Sulfide and Persulfide Donor P*: Insights into Hydrogen Sulfide Signaling. [Abstract]2021 Jun 29;10(7):1049. PMID: 34209813 -
Int J Nanomedicine
Copper Nanoparticles Induce Oxidative Stress via the Heme Oxygenase 1 Signaling Pathway in vitro Studies. [Abstract]2021 Feb 26:16:1565-1573. PMID: 33664571 -
Fluids Barriers CNS
Reactive astrocyte-derived exosomes enhance intracranial lymphatic drainage in mice after intracranial hemorrhage. [Abstract]2025 Apr 14;22(1):37. PMID: 40229887 -
Ecotoxicol Environ Saf
Uncovering the effects of ATBC plasticizers on intracerebral hemorrhage outcomes based on network toxicology and in vitro and in vivo experimental validation. [Abstract]2025 Jun 6:300:118486. PMID: 40482445 -
Biomater Adv
Platelet membrane-based bionic nanocarrier-targeted delivery of desferrioxamine mitigates secondary neurological damage in intracerebral hemorrhage. [Abstract]2026 Mar:180:214595. PMID: 41223662 -
J Invest Dermatol
CAMKK2 Defines Ferroptosis Sensitivity of Melanoma Cells by Regulating AMPK‒NRF2 Pathway. [Abstract]2022 Jan;142(1):189-200.e8. PMID: 34242660 -
Cell Prolif
Nuclear receptor Rev-erbα alleviates intervertebral disc degeneration by recruiting NCoR-HDAC3 co-repressor and inhibiting NLRP3 inflammasome. [Abstract]2024 Jul 24:e13720. PMID: 39045886 -
Am J Chin Med
Quercetin Alleviates Endothelial Dysfunction in Atherosclerosis by Inhibiting Ferroptosis Through PACS2/HMOX-1 Pathway. [Abstract]2026;54(2):603-625. PMID: 41692703 -
Mar Drugs
Xanthocillin X Dimethyl Ether Exhibits Anti-Proliferative Effect on Triple-Negative Breast Cancer by Depletion of Mitochondrial Heme. [Abstract]2025 Mar 28;23(4):146. PMID: 40278267 -
Inflamm Res
Minocycline alleviates microglia ferroptosis by inhibiting HO-1 during cerebral ischemia-reperfusion injury. [Abstract]2024 Oct;73(10):1727-1745. PMID: 39112649 -
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CNS Neurosci Ther
Network Pharmacology-Based and Experimental Validation Elucidate the Target Mechanism of Vinorine in Ameliorating Secondary Brain Injury After Intracerebral Hemorrhage. [Abstract]2025 Sep;31(9):e70609. PMID: 40994248 -
PLoS Pathog
Bafilomycin A1 is a promising therapeutic agent against T. spiralis infection by inhibiting the heme-transporting ATP6V0C/HRG-1 complex. [Abstract]2026 Mar 16;22(3):e1014042. PMID: 41838682 -
Int J Mol Sci
Cucurbitacin I Reverses Tumor-Associated Macrophage Polarization to Affect Cancer Cell Metastasis. [Abstract]2023 Nov 2;24(21):15920. PMID: 37958903 -
Biomolecules
Active Estrogen-Succinate Metabolism Promotes Heme Accumulation and Increases the Proliferative and Invasive Potential of Endometrial Cancer Cells. [Abstract]2023 Jul 10;13(7):1097. PMID: 37509133 -
Front Pharmacol
2021 Mar 19:12:629379. PMID: 33815110 -
Eur J Pharmacol
2025 Oct 11:1007:178232. PMID: 41082995 -
Int Immunopharmacol
Macrophage-specific deletion of HO-1 aggravates radiation-induced lung injury through an PP2A-dependent manner. [Abstract]2025 Sep 15:166:115548. PMID: 40957171 -
Int Immunopharmacol
Dracorhodin perochlorate sensitizes colorectal cancer to ferroptosis by activating HMOX1 and inhibiting the SLC7A11/GPX4 axis. [Abstract]2025 May 12:158:114827. PMID: 40359890 -
Eur J Pharmacol
Oridonin promotes RSL3-induced ferroptosis in breast cancer cells by regulating the oxidative stress signaling pathway JNK/Nrf2/HO-1. [Abstract]2024 Jul 5:974:176620. PMID: 38685305 -
Eur J Pharm Sci
Biophysical and docking study on the interaction of anticancer drugs encorafenib and binimetinib with human serum albumin. [Abstract]2023 Oct 1:189:106550. PMID: 37527692 -
Eur J Pharmacol
Overexpressed mitogen-and stress-activated protein kinase 1 promotes the resistance of cytarabine in acute myeloid leukemia through brahma related gene 1-mediated upregulation of heme oxygenase-1. [Abstract]2022 Feb 15:917:174722. PMID: 34953799 -
Int Immunopharmacol
MicroRNA-152 attenuates neuroinflammation in intracerebral hemorrhage by inhibiting thioredoxin interacting protein (TXNIP)-mediated NLRP3 inflammasome activation. [Abstract]2020 Mar;80:106141. PMID: 31982825 -
Mol Pharm
Molecular Interactions of Cobimetinib and Vemurafenib with Human Serum Albumin: a Comparative Biophysical and Computational Analysis. [Abstract]2025 Aug 4;22(8):4969-4982. PMID: 40662409 -
ACS Omega
2024 Dec 19;10(1):1188-1197. PMID: 39829454 -
FASEB J
TREM-1 Promotes Microglial Pyroptosis and Mitochondrial Fission in Intracerebral Hemorrhage via the PI3K/AKT Pathway. [Abstract]2025 Oct 15;39(19):e71115. PMID: 41065767 -
Drug Dev Res
Oleanolic Acid Alleviates Neuronal Ferroptosis in Subarachnoid Hemorrhage by Inhibiting KEAP1-Nrf2 and NF-κB Pathways. [Abstract]2025 May;86(3):e70105. PMID: 40358968 -
Drug Dev Res
GSTO2 ameliorates human neuroblastoma cell apoptosis, inflammation, ferroptosis, and oxidative stress by upregulating GPX4 expression in intracerebral hemorrhage. [Abstract]2024 Sep;85(6):e22245. PMID: 39154227 -
J Inflamm Res
Spermidine Exerts Neuroprotective Effects Following Intracerebral Hemorrhage in Mice Through Anti-Inflammation and Blood-Brain Barrier Protection. [Abstract]2025 Jun 7:18:7401-7417. PMID: 40503490 -
Neurochem Int
2026 Mar 28:196:106150. PMID: 41912085 -
J Neurochem
Spinal Nrf2 translocation may inhibit neuronal NF-κB activation and alleviate allodynia in a rat model of bone cancer pain. [Abstract]2021 Sep;158(5):1110-1130. PMID: 34254317 -
Fish Shellfish Immunol
Dietary 5-aminolevulinic acid induces strain-specific effects on shell color and innate immunity in Pacific oyster (Crassostrea gigas) through divergent porphyrin metabolism. [Abstract]2026 May:172:111209. PMID: 41690531 -
Sci Rep
Edaravone dexborneol provides neuroprotective benefits by suppressing ferroptosis in experimental intracerebral hemorrhage. [Abstract]2025 May 13;15(1):16595. PMID: 40360664 -
ACS Chem Neurosci
Mitochondrial Inhibitor Rotenone Triggers and Enhances Neuronal Ferroptosis Following Intracerebral Hemorrhage. [Abstract]2023 Mar 15;14(6):1071-1079. PMID: 36848438 -
Neurochem Res
Kaempferol Plays a Neuroprotection Role by Alleviating Oxidative Stress via AKT/Nrf2/HO-1 Pathway and Inhibiting Apoptosis in Intracerebral Hemorrhage. [Abstract]2025 Sep 6;50(5):291. PMID: 40913617 -
Reproduction
HIF1A-induced heme oxygenase 1 promotes the survival of decidual stromal cells against excess heme-mediated oxidative stress. [Abstract]2021 Dec 27;163(1):33-43. PMID: 34874275 -
Exp Cell Res
2022 Jan 1;410(1):112946. PMID: 34826424 -
PLoS Negl Trop Dis
The phytochemical arbutin exerts potent anti-Toxoplasma effects through activation of cell-autonomous defense mechanisms while attenuating inflammation. [Abstract]2025 Dec 11;19(12):e0013815. PMID: 41379884 -
Appl Biochem Biotechnol
Bone Marrow Mesenchymal Stem Cell-Derived Exosomes Attenuate Secondary Brain Injury After Intracerebral Hemorrhage in Rats by Modulating the SIRT6/Notch1/NF-κB Signaling. [Abstract]2026 Mar 23. PMID: 41870687 -
Cytotherapy
Heme oxygenase-1 inhibits the cytotoxicity of natural killer cells to acute myeloid leukemia by downregulating human leukocyte antigen-C. [Abstract]2023 Jul;25(7):728-738. PMID: 36890092 -
Arch Biochem Biophys
RNA binding motif protein 15 promotes intracerebral hemorrhage progression by regulating mitophagy through E2F transcription factor 1. [Abstract]2026 Apr:778:110746. PMID: 41571175 -
J Biochem Mol Toxicol
Anisodamine Ameliorates Traumatic Brain Injury-Induced Neuroinflammation and Neurological Injury in the Animal Model. [Abstract]2026 Jan;40(1):e70677. PMID: 41486509 -
J Biochem Mol Toxicol
Heme Oxygenase-1 Regulates the JAK/STAT Pathway to Inhibit Ferroptosis in Metabolic Dysfunction-Associated Steatotic Liver Disease. [Abstract]2026 Jan;40(1):e70661. PMID: 41486516 -
Front Neurol
MicroRNA-21 Overexpression Promotes the Neuroprotective Efficacy of Mesenchymal Stem Cells for Treatment of Intracerebral Hemorrhage. [Abstract]2018 Nov 6:9:931. PMID: 30459705 -
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Brain Res
Silencing of METTL14 attenuates experimental intracerebral hemorrhage by suppressing TFRC-mediated ferroptosis. [Abstract]2025 Nov 1:1866:149927. PMID: 40907591 -
Clin Exp Pharmacol Physiol
Hemin mitigates contrast-induced nephropathy by inhibiting ferroptosis via HO-1/Nrf2/GPX4 pathway. [Abstract]2022 Aug;49(8):858-870. PMID: 35598290 -
Am J Reprod Immunol
Active Heme Metabolism Suppresses Macrophage Phagocytosis via the TLR4/Type I IFN Signaling/CD36 in Uterine Endometrial Cancer. [Abstract]2024 Aug;92(2):e13916. PMID: 39166450 -
Stem Cells Dev
Human Neural Stem Cell Secretome Inhibits Neuron Heme Uptake and Ferroptosis in Intracerebral Hemorrhage through Nrf-2 Signaling Pathway. [Abstract]2023 Jun;32(11-12):346-363. PMID: 36960702 -
J Vis Exp
Neuronal Ferroptosis Attenuation by RKIP Inhibition Following Spontaneous Intracerebral Hemorrhage via NRF2/HO-1 Pathway Activation. [Abstract]2025 Oct 31:(224). PMID: 41247931 -
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Solvent & Solubility
1M NaOH : 6.67 mg/mL (10.23 mM; ultrasonic and adjust pH to 12 with NaOH)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
For the cell proliferation assay, cells are seeded into 96 well (5-12.5×104 cells per mL according to the cell line) and kept at 37°C and 5 % CO2. After 24 h, cells are treated with statins or/and Hemin, followed by the MTT test as a general cell proliferation assay[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Mice[2]
Eight- to ten-week-old male BABL/c mice are used for the ischemia-reperfusion (I/R) experiments. The animals are divided into five groups as follows: (1) the sham group undergo isolation of the bilateral renal arteries without clamping; (2) the vehicle group receive an intraperitoneal (i.p.) injection of 4 mL/kg PBS as a vehicle control (with IRI); (3) the Hemin-preconditioned group receive Hemin, a potent inducer of HO-1, at 100 μmol/kg i.p.; (4) the Hemin plus ZnPP group receive zinc protoporphyrin IX, an inhibitor of HO-1 activity, at 5 mg/kg i.p. 6 h after receiving 100 μmol/kg Hemin i.p.; and (5) the Hemin plus PD98059 group receive PD98059, an inhibitor of ERK1/2 activity, at 10 mg/kg i.p. 6 h after receiving 100 μmol/kg Hemin i.p. Both inhibitors are administered i.p. 2 h before I/R, whereas Hemin was administered 8 h before I/R.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (282 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Vanova K, et al. Heme oxygenase is not involved in the anti-proliferative effects of statins on pancreatic cancer cells. BMC Cancer. 2016 May 12;16:309. [Content Brief]
[2]. Chen HH, et al. Heme oxygenase-1 ameliorates kidney ischemia-reperfusion injury in mice through extracellular signal-regulated kinase 1/2-enhanced tubular epithelium proliferation. Biochim Biophys Acta. 2015 Oct;1852(10 Pt A):2195-201. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| 1M NaOH | 1 mM | 1.5339 mL | 7.6694 mL | 15.3388 mL | 38.3471 mL |
| 5 mM | 0.3068 mL | 1.5339 mL | 3.0678 mL | 7.6694 mL | |
| 10 mM | 0.1534 mL | 0.7669 mL | 1.5339 mL | 3.8347 mL |
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