1. Autophagy
    Apoptosis
  2. Autophagy
    Mitophagy
    Ferroptosis
  3. Hemin

Hemin (Synonyms: Hemin chloride)

Cat. No.: HY-19424 Purity: >98.0%
Handling Instructions

Hemin is an iron-containing porphyrin. Hemin is an Heme oxygenase (HO)-1 inducer.

For research use only. We do not sell to patients.

Hemin Chemical Structure

Hemin Chemical Structure

CAS No. : 16009-13-5

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply Now  
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
500 mg USD 50 In-stock
Estimated Time of Arrival: December 31
1 g USD 60 In-stock
Estimated Time of Arrival: December 31
5 g USD 120 In-stock
Estimated Time of Arrival: December 31
10 g   Get quote  
50 g   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 3 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Hemin is an iron-containing porphyrin. Hemin is an Heme oxygenase (HO)-1 inducer.

IC50 & Target

Heme oxygenase[1]

In Vitro

Hemin and PGJ2, used as positive controls, strongly increase both expression and activity of HMOX after 4 and 12 h, respectively. Indeed, a significant effect is found of 30 μM Hemin on cell proliferation in all used cell lines after 48 h, which is dose-dependent. Hemin treatment decreases cell proliferation to 62±5 %, 51±3 %, and 38±8 % in PA-TU-8902, BxPC-3 and MiaPaCa-2 cancer cells, respectively, with p<0.0001 for all comparisons. Furthermore, enhancement of anti-proliferative effects of statins is observed by Hemin, documented as decreased cell proliferation after 48 h of co-treatment[1].

In Vivo

Following the i.p. administration of Hemin (100 μmol/kg), the HO-1 level in the renal cortex begins to increase gradually. The HO-1 level reaches its peak 24 h after Hemin preconditioning. HO-1 is expressed mainly in the renal tubules. The HO-2 level in the kidney does not change following Hemin preconditioning[2].

Clinical Trial
Molecular Weight

651.94

Formula

C₃₄H₃₂ClFeN₄O₄

CAS No.

16009-13-5

SMILES

[Cl-][Fe+3]123[N-]4C5=C(CCC([O-])=O)C(C)=C4C=C(C(C=C)=C6C)[N]1=C6C=C(C(C=C)=C7C)[N-]2C7=CC(C(C)=C8CCC([O-])=O)=[N]3C8=C5.[H+].[H+]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 17.33 mg/mL (26.58 mM; Need ultrasonic)

NH4OH : 2.5 mg/mL (3.83 mM; ultrasonic and adjust pH to 11 with H2O)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5339 mL 7.6694 mL 15.3388 mL
5 mM 0.3068 mL 1.5339 mL 3.0678 mL
10 mM 0.1534 mL 0.7669 mL 1.5339 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 1.73 mg/mL (2.65 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 1.73 mg/mL (2.65 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[1]

For the cell proliferation assay, cells are seeded into 96 well (5-12.5×104 cells per mL according to the cell line) and kept at 37°C and 5 % CO2. After 24 h, cells are treated with statins or/and Hemin, followed by the MTT test as a general cell proliferation assay[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2]

Mice[2]
Eight- to ten-week-old male BABL/c mice are used for the ischemia-reperfusion (I/R) experiments. The animals are divided into five groups as follows: (1) the sham group undergo isolation of the bilateral renal arteries without clamping; (2) the vehicle group receive an intraperitoneal (i.p.) injection of 4 mL/kg PBS as a vehicle control (with IRI); (3) the Hemin-preconditioned group receive Hemin, a potent inducer of HO-1, at 100 μmol/kg i.p.; (4) the Hemin plus ZnPP group receive zinc protoporphyrin IX, an inhibitor of HO-1 activity, at 5 mg/kg i.p. 6 h after receiving 100 μmol/kg Hemin i.p.; and (5) the Hemin plus PD98059 group receive PD98059, an inhibitor of ERK1/2 activity, at 10 mg/kg i.p. 6 h after receiving 100 μmol/kg Hemin i.p. Both inhibitors are administered i.p. 2 h before I/R, whereas Hemin was administered 8 h before I/R.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: >98.0%

  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

HeminAutophagyMitophagyFerroptosisMitochondrial AutophagyInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Hemin
Cat. No.:
HY-19424
Quantity:
MCE Japan Authorized Agent: