2. Academic Validation
  3. Huaiqihuang (HQH) protects podocytes from high glucose-induced apoptosis and inflammation response by regulating PI3K/AKT/mTOR pathway

Huaiqihuang (HQH) protects podocytes from high glucose-induced apoptosis and inflammation response by regulating PI3K/AKT/mTOR pathway

  • Arch Physiol Biochem. 2025 Apr;131(2):285-292. doi: 10.1080/13813455.2024.2407552.
Peipei Zhang 1 2,  Zhilong Liu 3,  Guiqiao Ma 2 4,  Junwei Wang 2 4,  Jing Shao 1 2,  Chaojing Ma 1 2,  Liping Wang 1 2,  Chanjuan Ma 1 2 4 5
Affiliations

Affiliations

  • 1 Department of Nephrology, The Fifth Clinical Medical College of Shanxi Medical University, Fifth Hospital of Shanxi Medical University, Taiyuan, PR China.
  • 2 Shanxi Provincial Key Laboratory of Kidney Disease, Shanxi Provincial People's Hospital, Taiyuan, PR China.
  • 3 Hebei Key Laboratory of Integrated Traditional and Western Medicine in Osteoarthrosis Research (Preparing), Hebei Province Cangzhou Hospital of Integrated Traditional and Western Medicine, Cangzhou, China.
  • 4 The Third Clinical College, Shanxi University of Chinese Medicine, Jinzhong, PR China.
  • 5 Department of Nephrology, Shanxi Provincial People's Hospital, Taiyuan, PR China.
PMID: 39329410 DOI: 10.1080/13813455.2024.2407552
Abstract

Diabetic kidney disease (DKD) is one of the common microvascular complications of diabetes, and there are still lack of effective treatments. Huaiqihuang (HQH) is a kind of traditional Chinese medicine mixed preparation, which is mainly made of Trametes robiniophila Murr, Fructus Lycii, and Polygonatum sibiricumhas. It has been shown to be effective in the treatment of DKD, but the specific mechanism has not been fully elucidated. Our results showed that HQH increased the protein expressions of synaptopodin, podocin, WT-1, and Bcl-2, decreased the protein expressions of Bax and cleaved-casepase-3, and activated the NF-ĸB and PI3K/Akt/mTOR pathway in MPC5 cells exposed to high-glucose (HG). Real-Time PCR results showed that HQH reduced the mRNA expression of TNF-α, IL-1β, MCP-1, and IL-6. In conclusion, our results showed that HQH may attenuate podocyte injury by inhibiting MPC5 cell Apoptosis induced by HG and NF-κB-mediated inflammation response through activation of the PI3K/Akt/mTOR pathway.

Keywords

Diabetic kidney disease; Huaiqihuang; PI3K/AKT/mTOR; inflammation response; podocyte apoptosis.

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