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  3. Let-7f-5p Modulates Lipid Metabolism by Targeting Sterol Regulatory Element-Binding Protein 2 in Response to PRRSV Infection

Let-7f-5p Modulates Lipid Metabolism by Targeting Sterol Regulatory Element-Binding Protein 2 in Response to PRRSV Infection

  • Vet Sci. 2024 Aug 26;11(9):392. doi: 10.3390/vetsci11090392.
Dongfeng Jiang 1 2 3,  Liyu Yang 2 3,  Xiangge Meng 1,  Qiuliang Xu 2 3,  Xiang Zhou 1 4 5,  Bang Liu 1 4 5
Affiliations

Affiliations

  • 1 Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.
  • 2 College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China.
  • 3 Henan Institute of Pig Biotech Breeding, Zhengzhou 450046, China.
  • 4 The Engineering Technology Research Center of Hubei Province Local Pig Breed Improvement, Huazhong Agricultural University, Wuhan 430070, China.
  • 5 Hubei Hongshan Laboratory, Wuhan 430070, China.
PMID: 39330771 DOI: 10.3390/vetsci11090392
Abstract

Porcine reproductive and respiratory syndrome (PRRS) has caused substantial damage to the pig industry. MicroRNAs (miRNAs) were found to play crucial roles in modulating the pathogenesis of PRRS virus (PRRSV). In the present study, we revealed that PRRSV induced let-7f-5p to influence lipid metabolism to regulate PRRSV pathogenesis. A transcriptome analysis of PRRSV-infected PK15CD163 cells transfected with let-7f-5p mimics or negative control (NC) generated 1718 differentially expressed genes, which were primarily associated with lipid metabolism processes. Furthermore, the master regulator of lipogenesis SREBP2 was found to be directly targeted by let-7f-5p using a dual-luciferase reporter system and Western blotting. The findings demonstrate that let-7f-5p modulates lipogenesis by targeting SREBP2, providing novel insights into miRNA-mediated PRRSV pathogenesis and offering a potential Antiviral therapeutic target.

Keywords

PRRSV; RNA-seq; SREBP2; let-7f-5p; lipogenesis.

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