2. Academic Validation
  3. Protective effects of methylnissolin and methylnissolin-3-O-β-d-glucopyranoside on TNF-α-induced inflammation in human dermal fibroblasts

Protective effects of methylnissolin and methylnissolin-3-O-β-d-glucopyranoside on TNF-α-induced inflammation in human dermal fibroblasts

  • Toxicol In Vitro. 2025 Apr:104:106005. doi: 10.1016/j.tiv.2024.106005.
Yea Jung Choi 1,  Xiaohua Wu 2,  Sullim Lee 3,  Jae Sung Pyo 4,  Jaejin Cho 5,  Shugeng Cao 6,  Ki Sung Kang 7
Affiliations

Affiliations

  • 1 Department of Preventive Medicine, College of Korean Medicine, Gachon University, Seongnam 13120, Republic of Korea.
  • 2 Department of Pharmaceutical Sciences, Daniel K. Inouye College of Pharmacy, University of Hawaii at Hilo, Hilo, HI 96720, United States.
  • 3 Department of Life Science, College of Bio-Nano Technology, Gachon University, Seongnam 13120, Republic of Korea.
  • 4 College of Pharmacy, Kyungsung University, Busan 48434, Republic of Korea; Brain Busan 21 Plus Research Project Group, Kyungsung University, Busan 48434, Republic of Korea.
  • 5 Department of Dental Regenerative Biotechnology, School of Dentistry Seoul national University, Seoul, Republic of Korea.
  • 6 Department of Pharmaceutical Sciences, Daniel K. Inouye College of Pharmacy, University of Hawaii at Hilo, Hilo, HI 96720, United States; Cancer Biology Program, University of Hawaii Cancer Center, Honolulu, HI 96813, United States. Electronic address: [email protected].
  • 7 Department of Preventive Medicine, College of Korean Medicine, Gachon University, Seongnam 13120, Republic of Korea. Electronic address: [email protected].
PMID: 39746385 DOI: 10.1016/j.tiv.2024.106005
Abstract

Methylnissolin-3-O-β-d-glucopyranoside (MNG) and methylnissolin (MN) are pterocarpan derivatives that are found in Plants, such as Astragalus membranaceus. There are limited existing studies on the potential health benefits of MNG, and currently there is no evidence to suggest that MNG has any impact on skin-aging. Tumor necrosis factor-alpha (TNF-α) plays a significant role in skin aging by promoting chronic inflammation, damaging skin cells, and impairing the skin's natural repair mechanisms. Targeting TNF-α or its downstream signaling pathways may be a promising strategy for preventing or reversing skin-aging. We tested the effect of MNG and MN on skin-aging by inducing cell inflammation and oxidative stress with TNF-α in HDFs. MNG and MN significantly reduced the TNF-α-induced secretion of matrix metalloproteinase-1 (MMP-1). However, MNG has more beneficial compound for oral administration than MN in pharmacokinetics analysis. The mechanism underlying the anti-skin-aging effect of MNG is related to the suppression of TNF-α-induced Reactive Oxygen Species (ROS) generation and mitogen-activated protein kinase (MAPKs) phosphorylation. Our results suggest that MNG is a potential candidate for preventing skin-aging induced by TNF-α.

Keywords

Human dermal fibroblasts; Methylnissolin; Methylnissolin-3-O-β-D -glucopyranoside; Skin-aging; Tumor necrosis factor-α.

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