2. Academic Validation
  3. Paeoniflorin Inhibits Porcine Circovirus Type 2 Replication by Inhibiting Autophagy and Targeting AKT/mTOR Signaling

Paeoniflorin Inhibits Porcine Circovirus Type 2 Replication by Inhibiting Autophagy and Targeting AKT/mTOR Signaling

  • Vet Sci. 2025 Feb 2;12(2):117. doi: 10.3390/vetsci12020117.
Zhengchang Wu 1 2,  Luchen Yu 1,  Yueqing Hu 1,  Wenbin Bao 1,  Shenglong Wu 1
Affiliations

Affiliations

  • 1 Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
  • 2 International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of Jiangsu Higher Education Institutions, Yangzhou University, Yangzhou 225009, China.
PMID: 40005877 DOI: 10.3390/vetsci12020117
Abstract

Porcine circovirus type 2 (PCV2) is an important pathogen that leads to great economic losses to the swine industry. Paeoniflorin (PF), a novel plant extract, has been reported to have Antiviral properties. However, the role of paeoniflorin in regulating PCV2 replication remains unclear. Here, we used the CCK8 assay to demonstrate that PF within safe concentrations (0-275 mM) significantly inhibits PCV2 replication in a dose-dependent manner in porcine kidney cells. Subsequently, comparative transcriptome and functional verification revealed that PF probably inherits PCV2 replication via targeting Akt/mTOR signaling. Further experimental data show that the Akt/mTOR signaling pathway is highly relevant to Autophagy. Thus, experimental data from Western blot, qPCR, and the indirect immunofluorescence test indicate that PF inhibits PCV2 replication by inhibiting Autophagy by targeting the Akt/mTOR signaling pathway. Together, our results provide insight into the mechanism of paeoniflorin in regulating PCV2 replication and offer new ideas for the treatment of PCV2 Infection in pigs.

Keywords

AKT/mTOR signaling; autophagy; paeoniflorin; porcine circovirus type 2.

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