2. Academic Validation
  3. Flavonoids and phenolic acids from Lablab Flos Alba and their anti-inflammatory activity via NLRP3 inflammasome

Flavonoids and phenolic acids from Lablab Flos Alba and their anti-inflammatory activity via NLRP3 inflammasome

  • Fitoterapia. 2025 Jun:183:106533. doi: 10.1016/j.fitote.2025.106533.
Huimin Li 1,  Zhongwei Shi 1,  Wei Zhang 1,  Yijin Wu 2,  Qiannan Yang 2,  Jingya Ruan 1,  Yingying Wang 1,  Yi Zhang 3,  Tao Wang 4
Affiliations

Affiliations

  • 1 State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, 301617 Tianjin, China.
  • 2 Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, 301617 Tianjin, China.
  • 3 State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, 301617 Tianjin, China; Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, 301617 Tianjin, China. Electronic address: [email protected].
  • 4 State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, 301617 Tianjin, China; Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, 301617 Tianjin, China. Electronic address: [email protected].
PMID: 40233859 DOI: 10.1016/j.fitote.2025.106533
Abstract

The flowers of Dolichos lablab L. (Lablab Flos Alba), a dual-purpose medicinal and dietary plant, exhibit significant nutritional and bioactive properties, though their phytochemical basis remains poorly characterized. To address this, a detailed phytochemical investigation was carried out on it, and twelve new compounds, namely flosdolilabflavonosides A1-A5, B (1-6), flosdolilabphenosides A, B, C1, C2, D1, D2 (7-12), along with thirty-two known Flavonoids (13-44) were obtained. Twenty-six of the known isolates were reported for the first time in Lablab Flos Alba. And nine constituted new records for the Lablab genus. The effects of compounds 1-12 on the NLRP3 inflammasome were further explored by using LPS/Nigericin-stimulated bone marrow-derived macrophages (BMDMs) as in vitro model. Elisa assay revealed that compounds 6, 8, and 12 could significantly inhibit the release of interleukin-1β (IL-1β). Mechanistic studies via Western blotting demonstrated their dual regulatory effects: pretreatment with compounds 6, 8, and 12 downregulated NLRP3 and pro-IL-1β expression prior to LPS stimulation, while post-treatment with LPS/nigericin markedly reduced levels of cleaved IL-1β and cleaved Caspase-1. These findings indicate that the anti-inflammatory activity of compounds 6, 8, and 12 arises from their interference with both the priming phase and the activation phase of NLRP3 inflammasome signaling.

Keywords

Anti-inflammation; Flosdoliabflavonosides; Flosdolilabphenosides; Lablab Flos Alba; NLRP3 inflammasome signaling pathway.

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