2. Academic Validation
  3. Determination of reducing sugars in the nanomole range with tetrazolium blue

Determination of reducing sugars in the nanomole range with tetrazolium blue

  • J Biochem Biophys Methods. 1985 Aug;11(2-3):109-15. doi: 10.1016/0165-022x(85)90046-6.
C K Jue,  P N Lipke
PMID: 4031356 DOI: 10.1016/0165-022x(85)90046-6
Abstract

Addition of sodium potassium tartrate to basic solutions of tetrazolium blue [(2,2',5,5'-tetraphenyl-3,3'-dimethoxy 4,4'-biphenylene) ditetrazolium chloride] greatly improved their efficacy as colorimetric reagents for reducing sugar determination. This modification increased sensitivity and decreased reaction time. The modified reagent can determine as little as 1 nmol of neutral sugars as well as 2-amino and N-acetyl amino sugars.

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