Blue Tetrazolium
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Blue Tetrazolium is a tetrazolium salt chromogenic dye that can be reduced to a dark blue formazan product. Blue Tetrazolium is applicable for assays such as succinate dehydrogenase activity detection and reducing sugar quantification. Blue Tetrazolium also serves as a novel corrosion inhibitor for cold-rolled steel in hydrochloric acid solutions. Blue Tetrazolium has a broad wavelength range (480-600 nm), with the maximum absorbance observed at 540 nm.
For research use only. We do not sell to patients.
- Purity: 98.03%
- CAS No.: 1871-22-3
- Formula: C40H32Cl2N8O2
- Molecular Weight:727.64
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Storage:
RT, sealed storage, away from moisture and light.
In solvent -80°C, 1 year , -20°C, 6 months
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CHO | IC50 |
>100 μM
Compound: tetrazolium blue chloride
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Toxicity in CHO cells
Toxicity in CHO cells
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[PMID: 18078758] |
Blue tetrazolium (0.137-1.37 mM; 60 min) is used to determine succinate dehydrogenase (SDH) activity in permeabilized Saccharomyces cerevisiae Bc16a cells, and cell density shows a linear correlation with BTf formation[3].
Blue tetrazolium (0.68 mM; 30 min) can be chemically reduced to BTf, and the resulting product is soluble in DMSO for preparation of a quantitative standard curve[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1871-22-3
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Appearance Solid
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Molecular Weight 727.64
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Formula C40H32Cl2N8O2
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Color Light yellow to yellow
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SMILES
COC(C=C(C1=CC(OC)=C(N2[N+](C3=CC=CC=C3)=NC(C4=CC=CC=C4)=N2)C=C1)C=C5)=C5N6[N+](C7=CC=CC=C7)=NC(C8=CC=CC=C8)=N6.[Cl-].[Cl-]
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Synonyms
Tetrazolium blue
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
RT, sealed storage, away from moisture and light
In solvent -80°C 1 year -20°C 6 months
Purity & Documentation
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Data Sheet (269 KB)
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SDS (418 KB)
- English - EN (418 KB)
- Français - FR (418 KB)
- Deutsch - DE (418 KB)
- Norwegian - NO (418 KB)
- Español - ES (418 KB)
- Swedish - SV (418 KB)
- Italian - IT (418 KB)
- Korean - KR (418 KB)
- Portuguese - PT (418 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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