Blue Tetrazolium  (Synonyms: Tetrazolium blue)

Blue Tetrazolium is a tetrazolium salt chromogenic dye that can be reduced to a dark blue formazan product. Blue Tetrazolium is applicable for assays such as succinate dehydrogenase activity detection and reducing sugar quantification. Blue Tetrazolium also serves as a novel corrosion inhibitor for cold-rolled steel in hydrochloric acid solutions. Blue Tetrazolium has a broad wavelength range (480-600 nm), with the maximum absorbance observed at 540 nm^[1][2][3].

Blue tetrazolium (0.137-1.37 mM; 60 min) is used to determine succinate dehydrogenase (SDH) activity in permeabilized Saccharomyces cerevisiae Bc16a cells, and cell density shows a linear correlation with BTf formation^[3].
Blue tetrazolium (0.68 mM; 30 min) can be chemically reduced to BTf, and the resulting product is soluble in DMSO for preparation of a quantitative standard curve^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

727.64

C₄₀H₃₂Cl₂N₈O₂

1871-22-3

Solid

Light yellow to yellow

COC(C=C(C1=CC(OC)=C(N2[N+](C3=CC=CC=C3)=NC(C4=CC=CC=C4)=N2)C=C1)C=C5)=C5N6[N+](C7=CC=CC=C7)=NC(C8=CC=CC=C8)=N6.[Cl-].[Cl-]

Room temperature in continental US; may vary elsewhere.

RT, sealed storage, away from moisture and light

• [1]. Li X, et al. Blue tetrazolium as a novel corrosion inhibitor for cold rolled steel in hydrochloric acid solution. Corrosion Science, 2010, 52(9): 2786-2792.

  [2]. Jue CK, et al. Determination of reducing sugars in the nanomole range with tetrazolium blue. J Biochem Biophys Methods. 1985;11(2-3):109-115.  [Content Brief]

  [3]. Kregiel D, et al. Succinate dehydrogenase activity assay in situ with blue tetrazolium salt in crabtree-positive Saccharomyces cerevisiae strain. 2008.