2. Academic Validation
  3. Ultrafast ROS Scavenging Activity of Amur Maple Tree Extracts Confers Robust Cardioprotection for Myocardial Ischemia/Reperfusion Injury

Ultrafast ROS Scavenging Activity of Amur Maple Tree Extracts Confers Robust Cardioprotection for Myocardial Ischemia/Reperfusion Injury

  • Antioxidants (Basel). 2025 May 31;14(6):671. doi: 10.3390/antiox14060671.
Aoyang Pu 1 2,  Woo-Sup Sim 3,  Yuen-Kei Liem 1,  Yimin Lai 1,  Bong-Woo Park 4,  Kyoung-Tae Lee 5,  Hun-Jun Park 3 6 7,  Kiwon Ban 1 2
Affiliations

Affiliations

  • 1 Department of Biomedical Sciences, City University of Hong Kong, Kowloon, Hong Kong SAR, China.
  • 2 Tung Biomedical Sciences Centre, City University of Hong Kong, Hong Kong SAR, China.
  • 3 Department of Biomedicine & Health Sciences, The Catholic University of Korea, Seoul 06591, Republic of Korea.
  • 4 Department of Biomedical Informatics, College of Applied Life Sciences, Jeju National University, Jeju 63243, Republic of Korea.
  • 5 Forest Microbiology and Application Division, National Institute of Forest Science, Suwon 16631, Republic of Korea.
  • 6 Division of Cardiology, Department of Internal Medicine, Uijeongbu St. Mary's Hospital, Uijeongbu 11765, Republic of Korea.
  • 7 Cell Death Disease Research Center, The Catholic University of Korea, Seoul 06591, Republic of Korea.
PMID: 40563305 DOI: 10.3390/antiox14060671
Abstract

Ginnalin A (GA), a polyphenolic compound derived from amur maple trees, has been identified as a powerful scavenger of Reactive Oxygen Species (ROS). Recognizing the pivotal role of ROS in exacerbating secondary damage during myocardial ischemia-reperfusion injury (MIRI), we fractionated GA-enriched extracts from the leaves of the amur maple tree, Acer tataricum L. subsp. ginnala (Maxim.) Wesm., using common Solvents of dichloromethane (DCM) and ethyl acetate (EA). When co-administered for 30 min, the DCM- and EA-fractioned extracts effectively protected cardiomyocytes from H2O2-induced damage. ROS-sensitive probes indicated that treatment with ginnala extracts significantly reduced both intracellular and mitochondrial ROS levels. Instead of enhancing the activity of antioxidative Enzymes, the ginnala extracts acted as natural antioxidases, directly scavenging various ROS such as superoxide, H2O2, hydroxyl radical, and Fe2+ within just 20 min. In a MIRI rat model, the in vivo administration of ginnala extracts provided significant cardioprotection by preserving viable myocardia and enhancing cardiac functions. Additionally, treatment with ginnala extracts significantly reduced cardiac fibrosis and denatured Collagen. Our study suggests that the ultrafast ROS scavenging capability of ginnala extracts offers substantial heart protection during MIRI. Incorporating ginnala extracts as a pharmacological intervention during reperfusion could effectively mitigate ROS-induced cardiac injury.

Keywords

anti-ROS; anti-ferroptosis; cardioprotection; ischemia-reperfusion injury.

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