2. Academic Validation
  3. Chlamydomonas protein kinase MAK phosphorylates FAP256/CEP104 and regulates axonemal microtubule assembly

Chlamydomonas protein kinase MAK phosphorylates FAP256/CEP104 and regulates axonemal microtubule assembly

  • Proc Natl Acad Sci U S A. 2025 Nov 18;122(46):e2503094122. doi: 10.1073/pnas.2503094122.
Yi Zhang 1,  Xinjia Yang 1,  Junmin Pan 1 2
Affiliations

Affiliations

  • 1 MOE Key Laboratory of Protein Sciences, State Key Laboratory of Complex, Severe, and Rare Diseases, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
  • 2 Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, China.
PMID: 41231942 DOI: 10.1073/pnas.2503094122
Abstract

The formation of cilia involves the assembly of axonemal microtubules, the structural core of the organelle, from tubulins that are delivered by intraflagellar transport and diffusion. Whether and how this process is controlled are not clear. Here, we show that Chlamydomonas MAK (CrMAK), related to mammalian ICK/MAK, regulates axonemal microtubule assembly by phosphorylating FAP256/CEP104, a microtubule plus-end tracking protein. CrMAK, primarily present in cilia, associates with the axoneme via its C-terminal tail, and is enriched at the ciliary tip during active ciliary assembly. It is constitutively active during this process and acts downstream of a ciliary length-regulatory complex composed of LF1, LF3, and LF2, a CCRK homologue. Loss of CrMAK or its kinase activity leads to aciliated cells. CrMAK interacts with and phosphorylates FAP256/CEP104 and dephosphorylation of FAP256/CEP104 impairs ciliary assembly. These findings reveal a molecular mechanism governing the assembly of axonemal microtubules.

Keywords

CEP104/FAP256; MAK/ICK; RCKs; axonemal microtubules; ciliogenesis.

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