2. Academic Validation
  3. Directed rescue strategy for enhanced implant osteointegration in aged rats

Directed rescue strategy for enhanced implant osteointegration in aged rats

  • Nat Commun. 2025 Nov 24;16(1):10322. doi: 10.1038/s41467-025-65284-z.
Xuan Li # 1 2,  Xiao Jiang # 1,  Ye He 3,  Hongwei Xiong 1,  Qian Huang 1,  Kun Xu 1,  Xin-Xin Luo 1,  Kai Li 4,  Bailong Tao 4,  Kangkang Zha 5,  Jing Wu 1,  Peng Liu 1,  Kaiyong Cai 6
Affiliations

Affiliations

  • 1 Key Laboratory of Biorheological Science and Technology, Ministry of Education College of Bioengineering, Chongqing University, Chongqing, PR China.
  • 2 Life Sciences Institute, School of Life Sciences and Medical Engineering, Guangxi Medical University, Nanning, PR China.
  • 3 Thomas Lord Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC, USA.
  • 4 Laboratory Research Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China.
  • 5 Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
  • 6 Key Laboratory of Biorheological Science and Technology, Ministry of Education College of Bioengineering, Chongqing University, Chongqing, PR China. [email protected].
  • # Contributed equally.
PMID: 41285771 DOI: 10.1038/s41467-025-65284-z
Abstract

The elimination of senescent cells can enhance the osteointegration of implants in elderly patients. However, achieving specific clearance of senescent cells without adversely affecting the function of normal cells remains challenging. Here we show an implant surface modification technique to achieve specific clearance of locally senescent cells by modulating their metabolism. Our method involve modifying implants with BPTES, a Glutaminase 1 (GLS1) inhibitor, through π-π stacking with dopamine. This modification effectively induces intracellular acidosis in senescent mesenchymal stem cells (MSCs) through suppression of glutaminolysis. Simultaneously, poly(γ-glutamate) (PGA), modified by a layer-by-layer method, serve as a high-density carbon source coating, continuously supporting glutamine metabolism in MSCs without ammonia production. Our results show that modified implants significantly reduce the senescence level around implants and promote osteointegration in aged rats. These findings provide promising insights into the design and application of orthopedic implants for elderly patients.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-12683
    98.75%, Glutaminase Inhibitor