2. Academic Validation
  3. Negative regulation of reassortant canine influenza virus replication and key site identification in porcine and ferret bronchial epithelial cell lines

Negative regulation of reassortant canine influenza virus replication and key site identification in porcine and ferret bronchial epithelial cell lines

  • Virulence. 2025 Dec;16(1):2595767. doi: 10.1080/21505594.2025.2595767.
Jingjing Guo 1 2,  Bud Jung 3,  Sun-Woo Yoon 4,  Yongjie Liu 1,  Zhixin Feng 1 2,  Minjoo Yeom 5,  Woonsung Na 6,  Qi Xu 7,  Jong-Woo Lim 5,  Maoda Pang 8,  Fei Hao 1 2,  Rong Chen 1 2,  Daesub Song 5,  Xing Xie 1 2
Affiliations

Affiliations

  • 1 Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
  • 2 Key Laboratory for Veterinary Bio-Product Engineering, Ministry of Agriculture and Rural Affairs, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, P.R. China.
  • 3 Department of Pharmacy, Korea University, Sejong, Republic of Korea.
  • 4 College of Life Sciences and Health Welfare, Department of Vaccine Biotechnology, Andong National University, Andong-si, Republic of Korea.
  • 5 College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Republic of Korea.
  • 6 College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
  • 7 College of Animal Science and Technology, Yangzhou University, Yangzhou, P.R. China.
  • 8 Key Laboratory of Control Technology and Standard for Agro-Product Safety and Quality, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
PMID: 41307451 DOI: 10.1080/21505594.2025.2595767
Abstract

The segmented nature and high mutability of the Influenza Virus RNA genome facilitate rapid mutation and reassortment, allowing the virus to breach host barriers and migrate between different species, potentially leading to unpredictable influenza outbreaks. With dogs emerging as new natural hosts for Influenza Virus, vigilant surveillance and scientific prevention strategies are imperative. Here, based on our previous isolation of 21 strains, which are reassortments of the canine Influenza Virus (CIV) H3N2 (KR/07) with gene segments from the influenza A pandemic (H1N1) 2009 virus strain (CA/09), the replication kinetics of these reassortants in immortalized mammalian respiratory epithelial cell lines from swine and ferret named hTERT-PBECs and hTERT-FBECs, alongside induced changes in cytokine expression, were investigated. Reverse genetics was utilized to generate the reassortment H3N2 canine influenza rKR/07-PB2/NP, which contains the PB2 and NP segments from CA/09. The viral titer of rKR/07-PB2/NP was significantly lower than those of the parental viruses KR/07 and CA/09. In addition, rKR/07-PB2/NP notably decreased expression levels of interleukin-1β (IL-1β) and interleukin-10 (IL-10) in both immortal cells, particularly in hTERT-PBECs. Our findings not only contribute to the understanding and exploring cross-species transmission mechanisms of Influenza Virus, but also provide new ideas for prevention and treatment of CIV.

Keywords

Canine influenza virus; cytokine expression; gene segment reassortment; mammalian respiratory epithelial cell lines; reverse genetics; viral replication.

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