2. Academic Validation
  3. TMEM165 promotes glioblastoma progression through epithelial-mesenchymal transition-mediated invasion and temozolomide chemoresistance

TMEM165 promotes glioblastoma progression through epithelial-mesenchymal transition-mediated invasion and temozolomide chemoresistance

  • Int J Biol Macromol. 2026 Jan;335(Pt 1):149216. doi: 10.1016/j.ijbiomac.2025.149216.
Honglei Cheng 1,  Yueju Ding 1,  Xinan Shen 2,  Qing Wang 3,  Zhicheng Zhang 3,  Yun Wang 4,  Shenbo Chen 1,  Shenghua Zhuo 5,  Kai Wang 5,  Liangwang Yang 1,  Fufu Ji 3,  Zhiwu Fu 1,  Qing Lan 6,  Kun Yang 7
Affiliations

Affiliations

  • 1 Department of Neurosurgery, The First Affiliated Hospital, Hainan Medical University, Haikou, 570100, Hainan, China.
  • 2 Department of Radiotherapy & Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, 215000, Jiangsu, China.
  • 3 Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215000, Jiangsu, China.
  • 4 The First Clinical School, The First Affiliated Hospital, Hainan Medical University, Haikou, 570100, Hainan, China.
  • 5 International Center for Aging and Cancer, Hainan Academy of Medical Sciences, Hainan Medical University, 570100, Haikou, China.
  • 6 Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215000, Jiangsu, China.. Electronic address: [email protected].
  • 7 Department of Neurosurgery, The First Affiliated Hospital, Hainan Medical University, Haikou, 570100, Hainan, China.. Electronic address: [email protected].
PMID: 41308767 DOI: 10.1016/j.ijbiomac.2025.149216
Abstract

Gliomas are the most common primary malignant tumors of the central nervous system and are characterized by their highly aggressive nature and poor prognosis. This study elucidates the oncogenic role of transmembrane protein TMEM165 in glioblastoma and its therapeutic potential. Bioinformatics analysis revealed that high expression of N-linked glycosylation regulatory proteins predicts poor prognosis in glioma patients, with TMEM165 first reported here demonstrating significant associations with shorter survival, higher tumor grade, and IDH wild-type status. Enrichment analysis indicated activation of the epithelial-mesenchymal transition (EMT) pathway in TMEM165-high groups and a positive correlation with temozolomide (TMZ) resistance. In vivo and in vitro experiments demonstrated that TMEM165 promotes glioblastoma proliferation, migration, and invasion by modulating EMT-related proteins, while reducing its sensitivity to TMZ. These findings identify TMEM165 as a novel therapeutic target for glioblastoma.

Keywords

Epithelial–mesenchymal transition; Glioblastoma; Stem cell; TMEM165; TMZ resistance.

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