2. Academic Validation
  3. JOSD2 alleviates hypertensive renal disease through deubiquitinating AKT in renal tubular epithelial cells

JOSD2 alleviates hypertensive renal disease through deubiquitinating AKT in renal tubular epithelial cells

  • Cell Signal. 2025 Dec 16:139:112330. doi: 10.1016/j.cellsig.2025.112330.
Shijie Fan 1,  Ying Zhao 1,  Luyao Li 2,  Qingqing Zhao 3,  Ziming Fang 3,  Diyun Xu 3,  Jingjing Shao 4,  Yunjie Zhao 3,  Guang Liang 5,  Xuelin He 6,  Hong Zhu 7,  Yi Wang 8
Affiliations

Affiliations

  • 1 Department of Endocrinology, the First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China; Chemical Biology Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China.
  • 2 School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China.
  • 3 Chemical Biology Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China.
  • 4 School of Pharmacy, Hangzhou Normal University, Hangzhou, Zhejiang, China.
  • 5 Chemical Biology Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China; School of Pharmaceutical Sciences, Hangzhou Medical College, Hangzhou, Zhejiang, China.
  • 6 Kidney Disease Center, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. Electronic address: [email protected].
  • 7 Department of Endocrinology, the First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China. Electronic address: [email protected].
  • 8 Department of Endocrinology, the First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China; Chemical Biology Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China; School of Pharmacy, Hangzhou Normal University, Hangzhou, Zhejiang, China. Electronic address: [email protected].
PMID: 41412561 DOI: 10.1016/j.cellsig.2025.112330
Abstract

Hypertensive renal disease (HRD) is a significant driver of end-stage renal disease. Discovering novel therapeutic targets for HRD is essential for its prevention and treatment. Deubiquitinating Enzymes (DUBs) have shown increasing significance in renal diseases. Here, we investigated the role and mechanism of the DUB, Josephin domain-containing protein 2 (JOSD2), in HRD. HRD was induced in wild-type or Josd2 knockout mice via a 4-week chronic infusion of angiotensin II (Ang II). We found that deficiency of JOSD2 aggravated renal injury, epithelial-mesenchymal transition (EMT), and fibrosis in HRD mice. Single-cell RNA-seq analysis indicated that JOSD2 is mainly expressed in tubular epithelial cells (TECs) of proximal tubules. Notably, the specific overexpression of JOSD2 in renal TECs alleviated the detrimental effects in Ang II-induced HRD mice. Mechanistically, through mass spectrometry combined with co-immunoprecipitation analysis, we considered protein kinase B (Akt) as a potential substrate of JOSD2. JOSD2 deubiquitinated the K63-linked ubiquitin chain of Akt via its active site H125 and then enhanced p62-mediated autophagic degradation of Akt. This process reduced the Akt level in TECs, thereby ultimately reducing renal EMT and fibrosis. Our study elucidates the role of the JOSD2-AKT axis in HRD and suggests that JOSD2 may serve as a promising therapeutic target for HRD.

Keywords

AKT; EMT; Fibrosis; Hypertensive renal disease; JOSD2.

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