Preclinical characterisation of the protective capacity of an anti-nucleoprotein hRSV monoclonal antibody

Background: The human respiratory syncytial virus (hRSV) is a pathogen of global concern, causing significant morbidity and mortality, mainly in preterm infants. To date, all licenced monoclonal antibodies (mAbs) developed against hRSV have targeted its surface fusion or pre-fusion protein (F-hRSV).

Methods: We developed mAbs against the hRSV nucleoprotein (N-hRSV) as a complementary prophylactic treatment. Firstly, we produced a humanised murine mAb against N-hRSV, yielding four clones. Antibody affinity assays against the N-hRSV protein, pharmacokinetic analyses, and protection assays against pulmonary and neurological pathologies associated with hRSV Infection in an in vivo male and female BALB/c murine model were performed. In vitro assays were carried out to determine the mechanisms of action of the humanised anti-N-hRSV mAbs, which exhibit antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC).

Findings: Four humanised anti-N-hRSV mAb clones (P1-04H, P1-05D, P2-01A and P2-01D) were purified and showed high affinity for N-hRSV protein, as determined by SDS-PAGE and surface plasmon resonance, respectively. Pharmacokinetic analyses showed that two clones were detectable in the Animals up to 30 days post-immunisation. Furthermore, treating mice with the humanised anti-N-hRSV mAb 1 day before hRSV Infection reduced weight loss (P1-05D, ∗∗p = 0.0069; and P2-01D, p = 0.0875), clinical score (P1-05D, ∗∗p = 0.0052; P2-01A, ∗p = 0.04; P2-01D, ∗∗∗∗p < 0.0001), and pulmonary N-hRSV mRNA expression levels in hRSV serotype A-infected mice (P2-01A, p = 0.0612; P2-01D, p < 0.0613), while all Animals infected with hRSV serotype B showed reduced levels of N-hRSV expression (P1-04H, ∗∗p = 0.0022; P1-05D, ∗∗p = 0.0013; P2-01A, ∗∗p = 0.0013; and P2-01D, ∗∗p = 0.0013). Brain viral load was also decreased (P1-04H; P1-05D; P2-01A; and P2-01D, ∗∗∗∗p < 0.0001). 30 days post-infection, behavioural tests indicated protection against long-term neurological alterations, with reduced faecal boli (P1-04H; and P2-01D, ∗p = 0.0243), altered centre preference (P1-04H; P1-05D; and P2-01D, ∗∗∗∗p < 0.0001), and restoration of marble burying behaviour (clone P2-01D, ∗∗p = 0.0055). Finally, in vitro analyses showed that the humanised anti-N-hRSV mAb displayed ADCC and CDC mechanisms to target infected cells.

Interpretation: Our findings suggest that the humanised N-hRSV-specific mAbs displayed promising preclinical profiles by protecting against the pathology caused by hRSV Infection.

Funding: This study was supported by funding from the Millennium Institute on Immunology and Immunotherapy ANID ACE 210015 (CN09_016/ICN 2021_045; former P09/016-F, AMK); Biomedical Research Consortium CTU06 (AMK); FONDEF ID22I10252 (AMK and SMB); Fundación COPEC-UC 2019.R.1169 (AMK); FONDECYT Regular grant #1231866 (JAS), #1240971 (PAG), #1231905 (SMB), #1190830 and #1231851 (AMK). FONDECYT Iniciación grant #11221280 (KB). ANID FONDECYT Postdoctoral grant #3240624 (MAF). ANID Scholarship#21210662 (CAP), #21251811 (RAL), #21230429 (LRG), #21242058 (JTM), and #21221163 (BDV).

Antibody-dependent cellular cytotoxicity; Complement-dependent cytotoxicity; Human respiratory syncytial virus; Monoclonal antibodies; Nucleoprotein.