2. Academic Validation
  3. Large RNA polymerase II condensates are promoter-centric assemblies associated with early stages of transcription at all expressed genes

Large RNA polymerase II condensates are promoter-centric assemblies associated with early stages of transcription at all expressed genes

  • bioRxiv. 2026 May 25:2026.05.25.727590. doi: 10.64898/2026.05.25.727590.
Jelena V Bogdanović 1 2,  Jonah Galeota-Sprung 3,  Amoldeep S Kainth 2,  Filmon Medhanie 4,  Alisha Budhathoki 4,  Victoria Pappas 5,  Salman F Banani 6,  Jan-Hendrik Spille 4 7,  Alexander J Ruthenburg 2 8 9
Affiliations

Affiliations

  • 1 Department of Human Genetics, University of Chicago, Chicago, IL 60637, USA.
  • 2 Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA.
  • 3 Department of Biomedical Engineering, University of Illinois Chicago, Chicago, IL 60607, USA.
  • 4 Department of Physics, University of Illinois Chicago, Chicago, IL 60607, USA.
  • 5 Advanced Electron Microscopy Core, The University of Chicago, Chicago, Illinois.
  • 6 Department of Pathology, University of Chicago, Chicago, IL 60637, USA.
  • 7 NSF-Simons National Institute for Theory and Mathematics in Biology, Chicago, IL, USA.
  • 8 Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637, USA.
  • 9 Lead contact.
PMID: 42244752 DOI: 10.64898/2026.05.25.727590
Abstract

Transcriptional condensates concentrate the machinery required for RNA polymerase II mediated transcription. These structures range from numerous small, short-lived species, to a handful of larger, stable assemblages. Large condensates have been implicated in driving potent transcription of several super-enhancer regulated genes, yet the underlying mechanisms and the range of their client genes remain unclear. Here, we developed a biochemical approach which combines density gradient centrifugation and affinity purification to partially purify large transcriptional condensates from nuclei, allowing systematic characterization of their nucleic acid components. We find that transcriptional condensate isolates engage thousands of gene promoters and harbor the nascent transcriptome, but do not stably co-purify with distal enhancers. Binding patterns of RNA polymerase II within condensates suggest these structures could facilitate promoter escape and promoter-proximal pause release. Together, our work supports a promoter-centric condensate organization and paves the way towards understanding the functional link between condensate architecture and nascent transcription.

Keywords

Chromatin; Mediator; RNA polymerase II; Transcription; Transcriptional Condensates.

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