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Methyl Orange is a soluble azo dye commonly used as an acid-base indicator and for staining cells and tissue sections, as well as for dyeing textiles. Methyl Orange appears red at a pH of 3.1 and changes to bright yellow as the pH increases to 4.4. Methyl Orange (500 mg/L) exhibits cytotoxicity and can cause DNA damage.

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Methyl Orange

Methyl Orange 화학구조

CAS No. : 547-58-0

사이즈 가격 재고 수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
해외재고보유
Solution
10 mM * 1 mL in DMSO 해외재고보유
Solid
5 g 해외재고보유
10 g 해외재고보유
25 g 해외재고보유
50 g 해외재고보유
100 g 해외재고보유
> 100 g   견적 받기  

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 1 Customer Validation

Other Forms of Methyl Orange:

Top Publications Citing Use of Products
  • Biological Activity

  • 순도&문서

  • References

  • 고객리뷰

제품 설명

Methyl Orange is a soluble azo dye commonly used as an acid-base indicator and for staining cells and tissue sections, as well as for dyeing textiles. Methyl Orange appears red at a pH of 3.1 and changes to bright yellow as the pH increases to 4.4. Methyl Orange (500 mg/L) exhibits cytotoxicity and can cause DNA damage[1][2].

In Vitro

Guidelines (The following is our recommended experimental protocol. This protocol is for reference only, and specific operations should be adjusted according to your actual needs).
1. Acid-Base Indicator[2]
Methyl Orange exhibits a distinct color change at different pH values:
pH < 3.1: Red
pH 3.1 - 4.4: Orange
pH > 4.4: Bright Yellow

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

327.34

화학식

C14H14N3NaO3S

CAS No.
Appearance

Solid

Color

Orange to red

SMILES

O=S(C1=CC=C(C=C1)/N=N/C2=CC=C(C=C2)N(C)C)(O[Na])=O

선적

Room temperature in continental US; may vary elsewhere.

보관

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

용액&용해도
In Vitro: 

DMSO : 20 mg/mL (61.10 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0549 mL 15.2746 mL 30.5493 mL
5 mM 0.6110 mL 3.0549 mL 6.1099 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • 몰농도 계산기

  • 농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
순도&문서

Purity: 99.85%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0549 mL 15.2746 mL 30.5493 mL 76.3732 mL
5 mM 0.6110 mL 3.0549 mL 6.1099 mL 15.2746 mL
10 mM 0.3055 mL 1.5275 mL 3.0549 mL 7.6373 mL
15 mM 0.2037 mL 1.0183 mL 2.0366 mL 5.0915 mL
20 mM 0.1527 mL 0.7637 mL 1.5275 mL 3.8187 mL
25 mM 0.1222 mL 0.6110 mL 1.2220 mL 3.0549 mL
30 mM 0.1018 mL 0.5092 mL 1.0183 mL 2.5458 mL
40 mM 0.0764 mL 0.3819 mL 0.7637 mL 1.9093 mL
50 mM 0.0611 mL 0.3055 mL 0.6110 mL 1.5275 mL
60 mM 0.0509 mL 0.2546 mL 0.5092 mL 1.2729 mL
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Inquiry Information

상품명:
Methyl Orange
Cat. No.:
HY-118907
수량:
MCE Japan Authorized Agent: