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  2. Fluorescent Dye
  3. MSBN

MSBN is a highly selective fluorogenic probe for thiols. MSBN can be used to image thiols in live cells selectively and specifically label protein thiols with a signal to determine various reversible protein thiol modifications.

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MSBN

MSBN Chemische Struktur

CAS. Nr. : 135980-66-4

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO Auf Lager
Solid
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Beschreibung

MSBN is a highly selective fluorogenic probe for thiols. MSBN can be used to image thiols in live cells selectively and specifically label protein thiols with a signal to determine various reversible protein thiol modifications[1].

In Vitro

MSBN (5 µM; 0-60 min) shows a clean reaction with thiols (1 mM) to give a remarkable fluorescence signal[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molekulargewicht

331.39

Formel

C17H17NO4S

CAS. Nr.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C2=CC=CC3=C(S(=O)(C)=O)C=CC(C(N1CCCC)=O)=C32

Versand

Room temperature in continental US; may vary elsewhere.

Speicherung
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Lösungsmittel & Löslichkeit
In Vitro: 

DMSO : 100 mg/mL (301.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0176 mL 15.0880 mL 30.1759 mL
5 mM 0.6035 mL 3.0176 mL 6.0352 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molaritätsrechner

  • Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Reinheit & Dokumentation
Verweise

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0176 mL 15.0880 mL 30.1759 mL 75.4398 mL
5 mM 0.6035 mL 3.0176 mL 6.0352 mL 15.0880 mL
10 mM 0.3018 mL 1.5088 mL 3.0176 mL 7.5440 mL
15 mM 0.2012 mL 1.0059 mL 2.0117 mL 5.0293 mL
20 mM 0.1509 mL 0.7544 mL 1.5088 mL 3.7720 mL
25 mM 0.1207 mL 0.6035 mL 1.2070 mL 3.0176 mL
30 mM 0.1006 mL 0.5029 mL 1.0059 mL 2.5147 mL
40 mM 0.0754 mL 0.3772 mL 0.7544 mL 1.8860 mL
50 mM 0.0604 mL 0.3018 mL 0.6035 mL 1.5088 mL
60 mM 0.0503 mL 0.2515 mL 0.5029 mL 1.2573 mL
80 mM 0.0377 mL 0.1886 mL 0.3772 mL 0.9430 mL
100 mM 0.0302 mL 0.1509 mL 0.3018 mL 0.7544 mL
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MSBN Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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