Agrin Protein, Human (1073a.a, CHO, His)

Agrin is a heparan sulfate basal glycoprotein that is critical for the formation and maintenance of the neuromuscular junction (NMJ). It forms the AGRN-LRP4 receptor complex, activates MUSK and regulates gene transcription and AChR clusters in the postsynaptic membrane to form the NMJ. Agrin Protein, Human (1073a.a, CHO, His) is the recombinant human-derived Agrin protein, expressed by CHO , with C-6*His labeled tag.

Agrin is a heparan sulfate basal lamina glycoprotein crucial for the formation and maintenance of the neuromuscular junction (NMJ), orchestrating key events in postsynaptic differentiation. As a component of the AGRN-LRP4 receptor complex, it induces the phosphorylation and activation of MUSK, leading to NMJ formation by regulating processes such as gene transcription and AChR clustering in the postsynaptic membrane, where calcium ions play a pivotal role. The neuronal function of Agrin is intricately regulated by alternative splicing, glycan binding, and proteolytic processing. In neurons, it modulates calcium ion homeostasis, inducing an increase in cytoplasmic calcium ions. Agrin also acts differentially in the central nervous system (CNS) by inhibiting the alpha(3)-subtype of Na+/K+-ATPase, evoking depolarization at CNS synapses. The secreted isoform serves as a bridge between motor neurons and the basal lamina by binding laminin, while the transmembrane form, predominant in brain neurons, induces dendritic filopodia and synapse formation in mature hippocampal neurons through glycosaminoglycan chains and the action of Rho-family GTPases.

Measured by the ability of the immobilized protein to support the adhesion of U-87 MG human glioblastoma/astrocytoma cells. The ED₅₀ for this effect is 195.7 ng/ml, corresponding to a specific activity is 5.11×10^3 units/mg.

• 
  Measured by the ability of the immobilized protein to support the adhesion of U‑87 MG human glioblastoma/astrocytoma cells. The ED₅₀ for this effect is 195.7 ng/mL, corresponding to a specific activity is 5.11×10³ units/mg.

Human

CHO

C-6*His

O00468-1 (T30-R1102)

375790  [NCBI]

Full Length of Mature Agrin N-terminal 110 kDa subunit

TCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESLLDGGNKVVISGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVNPRTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGRDQCPEPCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFGALCEAETGRCVCPSECVALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCEADASAPATCAEMRCEFGARCVEESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISIQSLGPCQEAVAPSTHPTSASVTVTTPGLLLSQALPAPPGALPLAPSSTAHSQTTPPPSSRPRTTASVPRTTVWPVLTVPPTAPSPAPSLVASAFGESGSTDGSSDEELSGDQEASGGGSGGLEPLEGSSVATPGPPVER

approximately 155.89 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 500 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.