2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Phosphatase
  5. Alkaline Phosphatase
  6. Alkaline Phosphatase/ALPI Protein, Cynomolgus (HEK293, His)

Alkaline Phosphatase/ALPI Protein, Cynomolgus (HEK293, His)

Art. -Nr.: HY-P705497
Handling Instructions Technical Support

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  • Technical Parameters

  • Properties

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Species

Cynomolgus
Source

HEK293
Tag

C-His
Accession

A0A2K5VFX3 (I20-T501)
Gene ID

/
Molecular Construction
N-term
ALPI (I20-T501)
Accession # A0A2K5VFX3
His
C-term
Protein Length

Partial
Synonyms
Intestinal-type alkaline phosphatase; IAP; ALPI; Alkaline Phosphatase
AA Sequence

IIPAEEENPAFWNRQAAEALDAAKKLQPIQKVAKNLILFLGDGMGVPTVTAARILKGQKNGKLGPETPLAMDGFPYVALSKTYNVDRQVPDSAGTATAYLCGVKANYQTIGLSAAARYNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADMPASARQDGCQDIATQLISNVDIDVILGGGRKYMFPRGTPDPEYPSDASQNGIRLDGKYLVQEWLTKHQGARYVWNRTELMQVSLDQSVTHLMGLFEPGDMKYEINRDPTLDPSLLEMTEAALRLLSRNPGGFYLFVEGGRIDHGHHEGLAYHALTEAVMFDDAIERAGQLTSEDTLTLITADHSHVFSFGGYTFRGSSIFGLAPSKAQDSKAYTSILYGNGPGYVLNSGVRPDVNESESGSPDYRQQAAVPLSSETHGGEDVAVFARGPQAHLVHGVQEQSFVAHVMAFAACLEPYTACDLAPPAGTT

Predicted Molecular Mass
54 kDa.
Molekulargewicht

Approximately 65-80 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
Glycosylation
Yes
Reinheit

≥ 95%, as determined by reducing SDS-PAGE.
Appearance

Solution.
Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 150 mM NaCl, 20% Glycerol, pH7.5 with trehalose as protectant.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

N/A.
Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Versand

Shipping with dry ice.
Dokumentation
Handling Instructions (2659 KB)
Verweise

Alkaline Phosphatase/ALPI Protein, Cynomolgus (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Alkaline Phosphatase/ALPI Protein, Cynomolgus (HEK293, His)
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