Alkaline Phosphatase/ALPP Protein, Cynomolgus (HEK293, His)

Alkaline Phosphatase (ALPP) is an ubiquitous membrane-bound glycoprotein that catalyzes the hydrolysis of phosphate monoesters at basic pH values. Zinc and magnesium are essential cofactors of ALPP. ALPP down-regulates the secretory activities of the intrahepatic biliary epithelium^[1][2][3]. Alkaline Phosphatase/ALPP Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Alkaline Phosphatase/ALPP protein, expressed by HEK293 , with C-His labeled tag.

Alkaline Phosphatase (ALPP), a group of isoenzymes, are usually located on the outer layer of the cell membrane. ALPP catalyzes the hydrolysis of organic phosphate esters found in the extracellular space. ALPP is an ubiquitous membrane-bound glycoprotein that catalyzes the hydrolysis of phosphate monoesters at basic pH values^[1]. Zinc and magnesium are essential cofactors of ALPP. Besides, ALPP down-regulates the secretory activities of the intrahepatic biliary epithelium^[3].
ALPs are categorized into 2 types: tissue-specific and tissue-nonspecific. Tissue-specific ALPs are exclusively present in the intestine, placenta, and germinal tissue. Under specific conditions, the tissue-specific ALPs may also contribute to the circulating pool of serum ALP. More than 80% of the ALP in serum originates from the liver and bone^[2].

Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Cynomolgus Alkaline Phosphatase, His Tag with an affinity constant of 79.52 nM as determined in SPR assay (Biacore T200).

Cynomolgus

HEK293

C-8*His

XP_045223825.1 (I21-T503)

102133045  [NCBI]

Partial

IIPVEEENPDFWNRQAAEALGAAKKLQPIQTAAKNLIIFLGDGMGVSTVTAARILKGQKEEKLGPETPLAMDHFPYVALSKTYSVDKHVPDSAATATAYLCGVKGNFQTIGLSAAARYNQCNTTRGNEVVSVMNRAKKAGKSVGVVTTTRVQHASPAGAYAHTVNRNWYSDANMPGSARREGCKDIATQLISNMDIDVILGGGRKYMFRMGAPDPEYPHDYSQDGTRMDGKNLVQEWLAKHQGARYVWNRTELMQASLDPSVTHLMGLFEPGDMKYEIHRDPTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTEAVMFDDAIERAGQLTSEEDTLTLVTADHSHVFSFGAYPLRGSSIFGLAPGKAQDRKAYTALLYGNGPGYVLKDGARPDVTESESGSPEYRQQAAVPLDEETHAGEDVAVFARGPQAHLVHGVQEQSFVAHVMAFAACLEPYTACDLAPPAGTT

57-67 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 25 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH 7.5, 8% trehalose.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. /biology-dictionary/pc-3-cell.html  [Content Brief]