ANGPTL7/Angiopoietin-related 7 Protein, Canine (HEK293, His)

ANGPTL7/ Angiopoietin-related proteins are negative regulators of corneal angiogenesis and play an important role in maintaining the patency and transparency of corneal blood vessels. ANGPTL7 is regulated by SP1 transcription and regulates ANGPTL7 levels through the RhoA/ROCK signaling pathway mediating CLAN formation. ANGPTL7/Angiopoietin-related 7 Protein, Canine (HEK293, His) is the recombinant canine-derived ANGPTL7/Angiopoietin-related 7 protein, expressed by HEK293 , with C-His labeled tag.

ANGPTL7/ angiopoietin-related proteins play a crucial role in the formation and organization of the extracellular matrix. Studies have shown that, particularly in the eye, it acts as a medium for dexamethasone-induced matrix deposition in the trabecular network, the tissue necessary for the outflow of aqueous humor from the eye and maintenance of intraocular pressure. In addition, ANGPTL7 is a negative regulator of corneal angiogenesis and plays an important role in maintaining the patency and transparency of corneal blood vessels. ANGPTL7 is regulated by SP1 transcription and regulates ANGPTL7 levels through the RhoA/ROCK signaling pathway mediating CLAN formation^[1][2][3].

Measured by its binding ability in a functional ELISA. When Recombinant Human LILRB2 Protein is immobilized at 5 µg/mL (100µL/well) can bind Biotinylated Canine ANGPTL7 Protein. The ED₅₀ for this effect is 0.848 μg/mL.

Canine

HEK293

C-6*His

XP_852356.1/A0A8C0QI96 (Q27-P344)

608972  [NCBI]

Full Length of Mature Protein (with Propeptide)

QKPLKRKTPGQLKVATCCEEAKELKAQIANLTSLLSELSKKQERDWVSVVMQVMELESSTKRMESRLTDAESKYSEMNNQIDIMQLQAAQTVTQTSADAIYDCSSLYQKNYRISGVYKLPPDDFLGSPELEVFCDMETAGGGWTIIQRRKSGLVSFYRDWKQYKQGFGSIRGDFWLGNEHIHRLSRRPTRLRVEMEDWEGSVRYAEYSRFVLGNELNSYRLFLGNYSGNVGNDALLYHNNTAFSTKDKDNDNCLDKCAQLRKGGYWYNCCTDSNLNGVYYRLGEHNKHLDGITWYGWHGSSYSLRRVEMKIRPEDFQP

Approximately 35 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.