Animal-Free Enterokinase Protein, Bovine (P. pastoris, His)

Enterokinase proteins play a key role in initiating the activation of pancreatic proteolytic enzymes, including trypsin, chymotrypsin, and carboxypeptidase A. Its catalytic function converts trypsinogen to trypsin, triggering the cascade activation of chymotrypsinogen, procarboxypeptidase, and tropoelastin. Animal-Free Enterokinase Protein, Bovine (His) is the recombinant bovine-derived Enterokinase, expressed by P. pastoris, with C-His labeled tag. The total length of Animal-Free Enterokinase Protein, Bovine (His) is 235 a.a..

Enterokinase is a glycoprotein mainly present in the duodenal and jejunal mucosa. The core role of enterokinase is to activate digestive zymogens, converting trypsinogen into trypsin. The activated trypsin can not only directly decompose proteins but also further activate other zymogens such as chymotrypsinogen, procarboxypeptidase, and proelastase, forming a digestive enzyme cascade reaction to promote protein digestion^[1][2].

5 U/μL. Unit definition: One unit is defined as the amount of enzyme sufficient to cleave 50 μg of control fusion protein (~24 kDa) in 16 hours to 95% completion at 22 °C in a buffer containing 20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl₂, pH 7.4.

Bovine

P. pastoris

C-His

P98072-1

282009

Full Length of Enteropeptidase catalytic light chain

/

Approximately 40 kDa, based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 200 mM NaCl, 2 mM CaCl₂, 50% glycerol, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

< 1 EU/μg of protein by gel clotting method

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Mann NS, et al. Enterokinase. Proc Soc Exp Biol Med. 1994 Jun;206(2):114-8.  [Content Brief]

  [2]. Kitamoto Y, et al. Enterokinase, the initiator of intestinal digestion, is a mosaic protease composed of a distinctive assortment of domains. Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7588-92.  [Content Brief]