Animal-Free FGF-13 Protein, Human (His)

FGF-13 protein is a microtubule-binding protein that directly binds and stabilizes tubulin and affects microtubule polymerization. It exerts negative regulation on axonal and leading process branches, which are critical for neuronal polarization and migration in the cerebral cortex and hippocampus. Animal-Free FGF-13 Protein, Human (His) is the recombinant human-derived animal-FreeFGF-13 protein, expressed by E. coli , with C-His labeled tag. This product is for cell culture use only.

FGF-13, a microtubule-binding protein, exerts direct interaction with tubulin, participating in both the polymerization and stabilization of microtubules. This engagement in microtubule dynamics suggests a role in the refinement of axons by negatively regulating branching of axonal and leading processes. Additionally, FGF-13 plays a crucial role in the polarization and migration of neurons within the cerebral cortex and hippocampus. It further regulates voltage-gated sodium channel transport and function, influencing the activity of specific sodium channel isoforms. Beyond its microtubule-related functions, FGF-13 is implicated in the development of axonal initial segment-targeting inhibitory GABAergic synapses facilitated by chandelier neurons. Through its diverse interactions, including those with various sodium channels and MAPK signaling components, FGF-13 emerges as a multifaceted player in the intricate regulatory network governing neuronal processes and synaptic functions.

Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <160 ng/mL.

Human

E. coli

C-6*His

Q92913-1 (M1-T245)

2258  [NCBI]

Full Length of Isoform-1

MAAAIASSLIRQKRQAREREKSNACKCVSSPSKGKTSCDKNKLNVFSRVKLFGSKKRRRRRPEPQLKGIVTKLYSRQGYHLQLQADGTIDGTKDEDSTYTLFNLIPVGLRVVAIQGVQTKLYLAMNSEGYLYTSELFTPECKFKESVFENYYVTYSSMIYRQQQSGRGWYLGLNKEGEIMKGNHVKKNKPAAHFLPKPLKVAMYKEPSLHDLTEFSRSGSGTPTKSRSVSGVLNGGKSMSHNEST

Approximately 30 kDa, based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is recommended to reconstitute to a concentration of 100-200 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.