Animal-Free FGF-13 Protein, Human (His)

FGF-13 Protein, a microtubule-binding protein, directly binds and stabilizes tubulin, influencing microtubule polymerization. It negatively regulates axonal and leading process branching, crucial for neuron polarization and migration in the cerebral cortex and hippocampus. Additionally, FGF-13 regulates voltage-gated sodium channel transport and function, potentially impacting MAPK signaling. Interactions with SCN8A, SCN1A, SCN5A, SCN2A, and SCN11A suggest a role in modulating sodium channel activity and MAPK8IP2 scaffolding. Animal-Free FGF-13 Protein, Human (His) is the recombinant human-derived animal-FreeFGF-13 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free FGF-13 Protein, Human (His) is 245 a.a., with molecular weight of ~21.23 kDa.

FGF-13, a microtubule-binding protein, exerts direct interaction with tubulin, participating in both the polymerization and stabilization of microtubules. This engagement in microtubule dynamics suggests a role in the refinement of axons by negatively regulating branching of axonal and leading processes. Additionally, FGF-13 plays a crucial role in the polarization and migration of neurons within the cerebral cortex and hippocampus. It further regulates voltage-gated sodium channel transport and function, influencing the activity of specific sodium channel isoforms. Beyond its microtubule-related functions, FGF-13 is implicated in the development of axonal initial segment-targeting inhibitory GABAergic synapses facilitated by chandelier neurons. Through its diverse interactions, including those with various sodium channels and MAPK signaling components, FGF-13 emerges as a multifaceted player in the intricate regulatory network governing neuronal processes and synaptic functions.

Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <160 ng/mL.

Human

E. coli

C-His

Q92913-1 (M1-T245)

2258  [NCBI]

MAAAIASSLIRQKRQAREREKSNACKCVSSPSKGKTSCDKNKLNVFSRVKLFGSKKRRRRRPEPQLKGIVTKLYSRQGYHLQLQADGTIDGTKDEDSTYTLFNLIPVGLRVVAIQGVQTKLYLAMNSEGYLYTSELFTPECKFKESVFENYYVTYSSMIYRQQQSGRGWYLGLNKEGEIMKGNHVKKNKPAAHFLPKPLKVAMYKEPSLHDLTEFSRSGSGTPTKSRSVSGVLNGGKSMSHNEST

Approximately 21.23 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a solution containing 1X PBS, pH 7.4.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.