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  2. Cytokines and Growth Factors Animal-free Recombinant Proteins
  3. Animal-Free HDGF Protein, Human (His)

Animal-Free HDGF Protein, Human (His)

Cat. No.: HY-P700087AF
COA Handling Instructions

The HDGF protein acts as a transcriptional repressor and was previously thought to have mitogenic activity for fibroblasts and be a heparin-binding protein. However, recent studies have shown that it does not exhibit mitogenic activity for fibroblasts and does not bind heparin. Animal-Free HDGF Protein, Human (His) is the recombinant human-derived animal-FreeHDGF protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free HDGF Protein, Human (His) is 240 a.a., with molecular weight of ~29.60 kDa.

Animal-free recombinant proteins offer high consistency and stability, whithout using or contacting of any animal-derived materials and reagents.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
2 μg $50 In-stock
10 μg $139 In-stock
50 μg $389 In-stock
100 μg   Get quote  

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  • Biological Activity

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Description

The HDGF protein acts as a transcriptional repressor and was previously thought to have mitogenic activity for fibroblasts and be a heparin-binding protein. However, recent studies have shown that it does not exhibit mitogenic activity for fibroblasts and does not bind heparin. Animal-Free HDGF Protein, Human (His) is the recombinant human-derived animal-FreeHDGF protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free HDGF Protein, Human (His) is 240 a.a., with molecular weight of ~29.60 kDa.

Background

Hepatoma-derived growth factor (HDGF) exhibits a dual role as both a transcriptional repressor and a mitogenic factor for fibroblasts. While it acts as a repressor in transcriptional regulation, influencing cellular processes, it also demonstrates mitogenic activity, particularly for fibroblasts. HDGF has been identified as a heparin-binding protein, which suggests its involvement in interactions with heparin and potentially modulating cellular responses. However, it's noteworthy that conflicting reports exist, with some studies indicating that HDGF lacks mitogenic activity for fibroblasts and does not bind to heparin. These findings highlight the complexity of HDGF's functional repertoire, showcasing its diverse roles in transcriptional control, mitogenic signaling, and potential interactions with heparin.

Species

Human

Source

E. coli

Tag

C-His

Accession

P51858 (M1-L240)

Gene ID
Molecular Construction
N-term
HDGF (M1-L240)
Accession # P51858
His
C-term
Synonyms
Hepatoma-Derived Growth Factor; HDGF; High Mobility Group Protein 1-Kike 2; HMG-1L2; HDGF; HMG1L2
AA Sequence

MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCVEEPEPEPEAAEGDGDKKGNAEGSSDEEGKLVIDEPAKEKNEKGALKRRAGDLLEDSPKRPKEAENPEGEEKEAATLEVERPLPMEVEKNSTPSEPGSGRGPPQEEEEEEDEEEEATKEDAEAPGIRDHESL

Molecular Weight

Approximately 29.60 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Animal-Free HDGF Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Animal-Free HDGF Protein, Human (His)
Cat. No.:
HY-P700087AF
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