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  3. Animal-Free Intrinsic Factor/GIF Protein, Human (His)

Animal-Free Intrinsic Factor/GIF Protein, Human (His)

Cat. No.: HY-P700085AF
COA Handling Instructions

Intrinsic Factor (GIF) plays a vital role in cobalamin (Cbl) absorption in the ileum. The well-coordinated process involves the CBLIF-cobalamin complex interacting with the Cubilin (CUBN) receptor, leading to internalization through receptor-mediated endocytosis. GIF's crucial function ensures effective cobalamin absorption, essential for various physiological processes. The interaction between GIF and CUBN, particularly involving CUB domains, emphasizes the molecular intricacies of this essential vitamin uptake. Animal-Free Intrinsic Factor/GIF Protein, Human (His) is the recombinant human-derived animal-FreeIntrinsic Factor/GIF protein, expressed by E. coli, with C-His labeled tag. The total length of Animal-Free Intrinsic Factor/GIF Protein, Human (His) is 417 a.a., with molecular weight of ~46.23 kDa.

Animal-free recombinant proteins offer high consistency and stability, whithout using or contacting of any animal-derived materials and reagents.

For research use only. We do not sell to patients.

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Description

Intrinsic Factor (GIF) plays a vital role in cobalamin (Cbl) absorption in the ileum. The well-coordinated process involves the CBLIF-cobalamin complex interacting with the Cubilin (CUBN) receptor, leading to internalization through receptor-mediated endocytosis. GIF's crucial function ensures effective cobalamin absorption, essential for various physiological processes. The interaction between GIF and CUBN, particularly involving CUB domains, emphasizes the molecular intricacies of this essential vitamin uptake. Animal-Free Intrinsic Factor/GIF Protein, Human (His) is the recombinant human-derived animal-FreeIntrinsic Factor/GIF protein, expressed by E. coli, with C-His labeled tag. The total length of Animal-Free Intrinsic Factor/GIF Protein, Human (His) is 417 a.a., with molecular weight of ~46.23 kDa.

Background

Intrinsic Factor (GIF) stands as a key facilitator in the absorption of the essential vitamin cobalamin (Cbl) in the ileum. Its pivotal role unfolds through a well-orchestrated process, where the CBLIF-cobalamin complex, upon interaction with the Cubilin (CUBN) receptor, undergoes internalization via receptor-mediated endocytosis. This intricate interplay underscores the significance of GIF in ensuring the effective absorption of cobalamin, a crucial vitamin for various physiological processes. GIF's interaction with CUBN, particularly involving CUB domains, highlights the molecular intricacies governing this essential aspect of vitamin uptake.

Species

Human

Source

E. coli

Tag

C-His

Accession

P27352 (M1-Y417)

Gene ID
Molecular Construction
N-term
GIF (M1-Y417)
Accession # P27352
His
C-term
Synonyms
Gastric intrinsic factor; GIF; IF; Intrinsic factor; IFMH; INF; TCN3
AA Sequence

MAWFALYLLSLLWATAGTSTQTQSSCSVPSAQEPLVNGIQVLMENSVTSSAYPNPSILIAMNLAGAYNLKAQKLLTYQLMSSDNNDLTIGQLGLTIMALTSSCRDPGDKV

Molecular Weight

Approximately 46.23 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a solution containing 1X PBS, pH 8.0.

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Animal-Free Intrinsic Factor/GIF Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Animal-Free Intrinsic Factor/GIF Protein, Human (His)
Cat. No.:
HY-P700085AF
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